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Han Li,Hua Cao,Rong‑pei Yu,Zhen Miao,Ji‑hua Wang,Su‑Ping Qu,Qiang Yuan,Shenchong Li 한국원예학회 2018 Horticulture, Environment, and Biotechnology Vol.59 No.3
Paphiopedilum pacific shamrock is an orchid with high ornamental value. Understanding the mechanisms responsible for leaf color in albino mutants is important for ornamental development and breeding. In this study, we compared the leaf photosynthetic pigment content and transcriptome of albino mutants ppa01 and wild-type P. pacific shamrock. Photosynthetic pigment in mutants was less than 2% of the wild type and chl a/b was 60% less than the wild type. Transcriptome sequencing yielded 6.27 Gb and 5.67 Gb clean data from the mutant and wild-type leaves, respectively. De novo assembly yielded 104,763 unigenes with 15,400 greater than 1 kb in length. In unigene expression analysis, 3170 differentially expressed genes (DEGs) were identified with 2231 (70.38% of total DEGs) down-regulated. Results from GO and KEGG enrichment analysis, KEGG pathway analysis and qPCR suggest that the reduction of chloroplast biosynthesis and division in the mutant was due to low expression levels of ppGLK1 and ppFtsz. Mutants were associated with fewer chloroplasts in leaf cells, abnormal chloroplast structures, impaired chlorophyll biosynthesis, and thus reduced total chlorophyll and carotenoid contents. Furthermore, down-regulated expression of ppNYC1 reduced transformation of chlorophyll b into chlorophyll a, leading to a chl a/b decline. The research will guide future studies of leaf pigment mutations and the breeding of P. pacific shamrock.
Jiwei Ruan,용영록,Chunmei Yang,Guoxian Wang,Lifang Wu,Shenchong Li,Pang Tao,Chengfei Liao,Ji-Hua Wang 한국원예학회 2017 Horticulture, Environment, and Biotechnology Vol.58 No.6
Studies on the inheritance of day-neutrality, a highly desirable trait in strawberry (Fragaria×ananassa),have not been conclusive because flowering phenotypes were evaluated based on different materials and experimentalsites. In the present study, selfed and crossed progenies of Day-neutral (DN) ‘Albion’ and June-bearing (JB) ‘Akihime’cultivars, namely ‘Albion’ × ‘Albion’, ‘Akihime’ × ‘Akihime’, and ‘Akihime’ × ‘Albion’, were established in 2013and 2014. The flowering phenotype (DN or JB) of seedlings from each of the three progenies, as well as of parentalplants, was evaluated for two successive years (2015 and 2016) in Central Yunnan, China, and their segregationratios were used to elucidate day-neutrality inheritance. Single dominant gene inheritance was demonstrated forday-neutrality, although minor genes (from the JB parent ‘Akihime’) likely increase DN magnitude. During thejuvenile stage (i.e., long before the first inflorescence), the major gene controlling day-neutrality may not beexpressed. We provide basic information for DN gene mapping and cloning, as well as materials (progenies) fordomestic DN strawberry breeding.