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        In Situ Growth of Plasmonic Gold Nanoparticles for the Direct and Sensitive Colorimetric Assay of Glucose

        Shaohuang Weng,Feng Feng,Honghui Guo,Namei Wu,Yanjie Zheng,Zhen Lin,Xinhua Lin 대한화학회 2017 Bulletin of the Korean Chemical Society Vol.38 No.3

        Gold nanoparticles (Au NPs)-based colorimetric assay for the highly sensitive and selective detection of glucose has been conducted. The sensing, which was based on the in situ growth of Au NPs in the presence of glucose, was developed with the solution changing from colorless to red. This change could be observed with the naked eye or a ultraviolet–visible (UV–vis) spectrophotometer. Moreover, adding trace amounts of Tween-20 kept Au NPs well spaced. Under the optimal assay conditions, the absorbance of Au NPs at 550 nm was proportional to glucose concentration within 400–1800 μM with a linear regression equation, and the detection limit was 6.28 μM. Some common interfering compounds in beverages, including other sugars, food additives, and amino acids, did not affect glucose sensing. Therefore, the proposed Au NP-based colorimetric assay could be successfully used to determine glucose in beverages.

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        Positively Charged and pH-sensitive Carbon Dots for Fluorescence Detection of Copper Ion

        Feng Feng,Chenfang Miao,Yalan Zhang,Zhengjun Huang,Shaohuang Weng 대한화학회 2021 Bulletin of the Korean Chemical Society Vol.42 No.2

        As an essential trace element in the human body, copper ion (Cu2+) performs an important role in many fundamental physiological processes that need a facile and effective method for the monitoring of Cu2+ in organisms. Herein, a turn-off, facile fluorescent strategy for sensing Cu2+ was proposed using positively charged carbon dots (P-CDs) as probe. The P-CDs prepared from melting strategy exhibited stable, biocompatible, and pH-sensitive luminescent properties. P-CDs can be selectively quenched by Cu2+ for the direct detection of Cu2+. The reaction mechanism between Cu2+ and P-CDs was systematically investigated, illustrating that Cu2+ coordinated with the surface functional groups in P-CDs. The binding effect of Cu2+ and P-CDs caused the constant morphology and static quenching behavior of P-CDs. The promising accuracy of the testing results of the addition of Cu2+ in diluted serum using P-CDs implied their extended application in the biological field.

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