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A microlagal-based carbonaceous sensor for enzymatic determination of glucose in blood serum
Seyed Mostafa Jafari,Saeed Masoum,Seyed Ali Hosseini Tafreshi 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.101 No.-
In the present work, Chlorella vulgaris, a well-known microalga was used as a carbonic precursor, tosynthesis the water-soluble carbon dots (CDs) via hydrothermal method. To improve the properties,acidic carbon dots (ACDs) were also synthesized via ultrasoncation of microalgal biomass after acidichydrolysis (using diluted acidic solution). The aqueous solution of ACDs was used as a sensor for glucosedetermination based on glucose oxidase enzyme (GOx) reaction and fluorescence quenching of ACDs inthe presence of Fe3+ ions under the optimized conditions. The linear range of glucose in the blood serumwas measured from a range of 5 mM to 500 mM with a limit of detection (LOD) 2.84 mM. Additionally, thehydrogen peroxide, as the conventional reactive oxygen species (ROS) was determined based on theFenton reaction. The linear range of H2O2 was obtained from 10 mM to 200 mM with LOD of 975 nM. Inall of these applications, fluorescence quenching of ACDs was followed according to photo-inducedelectron transfer (PET) mechanism in the presence of generated Fe3+ ions by Fenton reaction.