한국산 살모사과 3종의 경쟁과 공간적 생태 -생태적 지위를 기반으로 한 모델과 지리정보시스템 적용-

도민석 ( Min Seock Do ),이진원 ( Jin Won Lee ),장환진 ( Hoan Jin Jang ),김대인 ( Dae In Kim ),유정칠 ( Jeong Chil Yoo ) 한국환경생태학회 2016 한국환경생태학회지 Vol.30 No.2

생물종의 경쟁과 공간적 분포, 생태적 지위의 관계에 대한 정보는 생물지리학적 분포형태를 이해하는 데 중요한 역할을 한다. 본 연구에서는 한국에 서식하고 있는 살모사과 3종(Gloydius ussuriensis, G. brevicaudus, G. saxatilis)에 대해 관찰자료와 종 분포 모델 기법을 바탕으로 분포 특성과 종의 생태적 지위를 산출하였고, 예측된 종 분포 모델들을 바탕으로 지리적 분포와 생태적 지위의 분화에 대한 종간 경쟁 관계의 영향을 추론하였다. 연구 결과 고도가 살모사들의분포에 가장 중요한 환경변수로 나타났으며, 그들이 분포한 고도는 그 지역의 기후와 상관관계를 나타냈다. 종간 생태적지위는 비교적 높게 중첩되어 있었지만, 예측된 3종의 분포형태는 태백산맥을 기준으로 차이를 나타냈다. 분포모델들을 중첩한 결과, 종간 중첩된 지역의 서식지는 대부분 산림지역으로, 전체 조사 지역에 비해 비교적 작은 범위가 중첩되어 접소적인 분포형태가 예측되었다. 또한 중첩된 지역에 분포한 개체수는 종간 양의 상관관계를 나타내고 있어, 이 지역에서 종간 경쟁이 심하지 않다는 것을 암시하고 있다. 결론적으로 한국에 서식하고 있는 살모사과 3종은 유사한 생태적지위를 차지하고 있지만, 직접적인 경쟁 없이 접소적인 분포형태를 띠고 있는 것으로 보인다. 향후 접소적인 분포형태를 일으키는 직접적인 요인을 알아내기 위해, 보다 자세한 생태학적, 행동학적 연구와 더불어 비교적 세밀한 격자 크기를 통한 다양한 지형변수(고도, 미소서식지 특성 등)들에 대한 연구가 진행될 필요가 있다. Knowledge of the relationships among interspecific competition, spatial distributions and ecological niches plays an important role in understanding biogeographical distribution patterns of species. In this study, the distributional characteristics and ecological niches of the three Viperidae species (Gloydius ussuriensis, G. brevicaudus, and G. saxatilis) in South Korea were determined based on observation data and species distribution model . The effects of interspecific competition on geographical distribution and the division of the ecological niches of the vipers were also examined based on the models of predicted species distribution. The results showed that altitude was the most important environmental variable for their distribution, and the altitudes at which these snakes were distributed correlated with the climate of that region. Although interspecific ecological niches are quite overlapped, their predicted distribution patternsvary by the Taebaek Mountains. When overlaying the distribution models, most of the overlapping habitats were forest areas, which were relatively less overlapped than were the entire research areas. Thus, a parapatric distribution pattern was expected. The abundance of species occurring sympatrically was positively correlated with each other , indicating the lack of serious interspecies competition in this region. In conclusion, although the three Viperidae species in South Korea occupy similar ecological niches, these snakes exhibit parapatric distribution patterns without direct competition. Further research on various geographic variables (e.g., altitude, microhabitat characteristics) using relatively fine grid sizes, as well as further detailed ecological and behavioral research, is needed to determine the causative factors for the parapatric distribution pattern.

국내 야생에서 발견되는 외래거북류의 분포 특성 - 강원도와 경상남도 지역을 중심으로 -

구교성,권세라,도민석,김수환,Koo, Kyo Soung,Kwon, Sera,Do, Min Seock,Kim, Suhwan 한국하천호수학회 2017 생태와 환경 Vol.50 No.3

Development of transportation is rapidly reducing the barriers between countries, but this is causing the easier migration of species than the past. Typically, exotic species are imported for the purpose of food, leather, and pets. However, it has been introduced into the wild through artificially or naturally paths, and recently they are become a main cause of ecosystem disturbance. In this study, we investigated exotic turtle species introduced into the wild and analyzed their distribution characteristics. As a result of filed surveys, totally 4 genus 8 species 62 individuals of exotic turtles were found from 126 reservoirs in Gangwon-do and Gyeongsangnam-do, South Korea. In particular, Trachemys scripta elegans showed relatively high frequency than other turtle species and the natural reproduction of T. s. elegans was confirmed in some areas. On the other hand, the frequency of discovery of exotic turtles except T. s. elegans was relatively low, and the range of the areas was limited. Especially, exotic turtles except T. s. elegans were mainly found in public places such as reservoirs in the park. As a result of analyzing the distribution characteristics of exotic turtles using geographic information system, the turtles' distribution showed a high correlation with the artificial factor such as "urban". In this study, we identified the distribution characteristics of exotic turtles in the Korean wild, and these results will be important data for understanding status the and establishing effective management methods for exotic species. 교통수단의 발달은 국가 간의 장벽을 빠르게 낮추고 있지만 이는 과거보다 쉬운 생물종 이동의 원인으로 작용하고 있다. 특히 수 많은 종류의 외래생물들이 식량과 가죽, 애완 등의 목적으로 수입되고 있으며, 인위적 혹은 자연적으로 야생에 유입되어 생태계 교란의 주범이 되고 있다. 본 연구에서는 대표적인 외래생물종인 붉은귀거북을 비롯한 외래거북을 대상으로 국내 생태계로의 유입현황을 파악하고, 이들의 분포 특성을 분석하고자 하였다. 강원도와 경상남도 지역에 위치한 총 126개의 저수지를 조사한 결과, 총 4속 8종 62개체의 외래거북들이 발견되었다. 그중 붉은귀거북의 발견 빈도가 상대적으로 높았으며, 일부 지역에서는 붉은귀거북의 자연 번식도 이루어지고 있는 것으로 확인되었다. 반면, 붉은귀거북을 제외한 다른 외래거북들의 발견 빈도는 비교적 낮았으며, 발견되는 지역의 범위는 제한적이었다. 특히 외래거북들은 공원 내 저수지와 같은 일반인의 접근이 비교적 높은 곳에서 주로 발견되었다. 지리정보시스템을 이용한 외래거북의 분포 특성을 분석한 결과, 외래거북의 발견은 시가지라는 인위적인 요소와 높은 연관성을 보였다. 이러한 연구 결과는 생태계에 유입되어 있는 외래거북류의 현황 파악과 동시에 이들을 효과적으로 관리하기 위한 방안을 수립의 중요한 근거가 될 것이다.

RAD51C-Deficient Cancer Cells Are Highly Sensitive to the PARP Inhibitor Olaparib

Min, Ahrum,Im, Seock-Ah,Yoon, Young-Kwang,Song, Sang-Hyun,Nam, Hyun-Jin,Hur, Hyung-Seok,Kim, Hwang-Phill,Lee, Kyung-Hun,Han, Sae-Won,Oh, Do-Youn,Kim, Tae-You,O'Connor, Mark J.,Kim, Woo-Ho,Bang, Yung-J American Association for Cancer Research 2013 Molecular cancer therapeutics Vol.12 No.6

<P>A PARP inhibitor is a rationally designed targeted therapy for cancers with impaired DNA repair abilities. RAD51C is a paralog of RAD51 that has an important role in the DNA damage response. We found that cell lines sensitive to a novel oral PARP inhibitor, olaparib, had low levels of RAD51C expression using microarray analysis, and we therefore hypothesized that low expression of RAD51C may hamper the DNA repair process, resulting in increased sensitivity to olaparib. Compared with the cells with normal RAD51C expression levels, RAD51C-deficient cancer cells were more sensitive to olaparib, and a higher proportion underwent cell death by inducing G<SUB>2</SUB>–M cell-cycle arrest and apoptosis. The restoration of RAD51C in a sensitive cell line caused attenuation of olaparib sensitivity. In contrast, silencing of RAD51C in a resistant cell line enhanced the sensitivity to olaparib, and the number of RAD51 foci decreased with ablated RAD51C expression. We also found the expression of RAD51C was downregulated in cancer cells due to epigenetic changes and RAD51C expression was low in some gastric cancer tissues. Furthermore, olaparib significantly suppressed RAD51C-deficient tumor growth in a xenograft model. In summary, RAD51C-deficient cancer cells are highly sensitive to olaparib and offer preclinical proof-of-principle that RAD51C deficiency may be considered a biomarker for predicting the antitumor effects of olaparib. <I>Mol Cancer Ther; 12(6); 865–77. ©2013 AACR</I>.</P>

AZD6738, A Novel Oral Inhibitor of ATR, Induces Synthetic Lethality with ATM Deficiency in Gastric Cancer Cells

Min, Ahrum,Im, Seock-Ah,Jang, Hyemin,Kim, Seongyeong,Lee, Miso,Kim, Debora Keunyoung,Yang, Yaewon,Kim, Hee-Jun,Lee, Kyung-Hun,Kim, Jin Won,Kim, Tae-Yong,Oh, Do-Youn,Brown, Jeff,Lau, Alan,O'Connor, Mar American Association for Cancer Research 2017 Molecular Cancer Therapeutics Vol.16 No.4

<P>These findings suggest synthetic lethality between ATR inhibition and ATM deficiency in gastric cancer cells. Further clinical studies on the interaction between AZD 6738 and ATM deficiency are warranted to develop novel treatment strategies for gastric cancer. (C)2017 AACR.</P>

Histone deacetylase inhibitor, suberoylanilide hydroxamic acid (SAHA), enhances anti-tumor effects of the poly (ADP-ribose) polymerase (PARP) inhibitor olaparib in triple-negative breast cancer cells

Min, Ahrum,Im, Seock-Ah,Kim, Debora Keunyoung,Song, Sang-Hyun,Kim, Hee-Jun,Lee, Kyung-Hun,Kim, Tae-Yong,Han, Sae-Won,Oh, Do-Youn,Kim, Tae-You,O’Connor, Mark J,Bang, Yung-Jue BioMed Central 2015 Breast cancer research Vol.17 No.-

<P><B>Introduction</B></P><P>Olaparib, a poly (ADP-ribose) polymerase (PARP) inhibitor, has been found to have therapeutic potential for treating cancers associated with impaired DNA repair capabilities, particularly those with deficiencies in the homologous recombination repair (HRR) pathway. Histone deacetylases (HDACs) are important for enabling functional HRR of DNA by regulating the expression of HRR-related genes and promoting the accurate assembly of HRR-directed sub-nuclear foci. Thus, HDAC inhibitors have recently emerged as a therapeutic agent for treating cancer by inhibiting DNA repair. Based on this, HDAC inhibition could be predicted to enhance the anti-tumor effect of PARP inhibitors in cancer cells by blocking the HRR pathway.</P><P><B>Methods</B></P><P>We determined whether suberoylanilide hydroxamic acid (SAHA), a HDAC inhibitor, could enhance the anti-tumor effects of olaparib on breast cancer cell lines using a cytotoxic assay, cell cycle analysis, and Western blotting. We evaluated how exposure to SAHA affects the expression of HRR-associated genes. The accumulation of DNA double strand breaks (DSBs) induced by combination treatment was assessed. Induction of autophagy was monitored by imaging green fluorescent protein-tagged microtubule-associated protein 1A/1B-light chain 3 (LC3) expression following co-treatment with olaparib and SAHA. These <I>in vitro</I> data were validated <I>in vivo</I> using a human breast cancer xenograft model.</P><P><B>Results</B></P><P>Triple-negative breast cancer cell (TNBC) lines showed heterogeneous responses to the PARP and HDAC inhibitors. Co-administration of olaparib and SAHA synergistically inhibited the growth of TNBC cells that expressed functional Phosphatase and tensin homolog (PTEN). This effect was associated with down-regulation of the proliferative signaling pathway, increased apoptotic and autophagic cell death, and accumulation of DNA damage. The combined anti-tumor effect of olaparib and SAHA was also observed in a xenograft model. These data suggest that PTEN expression in TNBC cells can sensitize the cell response to simultaneous inhibition of PARP and HDAC both <I>in vitro</I> and <I>in vivo</I>.</P><P><B>Conclusion</B></P><P>Our findings suggest that expression of functional PTEN may serve as a biomarker for selecting TNBC patients that would favorably respond to a combination of olaparib with SAHA. This provides a strong rationale for treating TNBC patients with PTEN expression with a combination therapy consisting of olaparib and SAHA.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (doi:10.1186/s13058-015-0534-y) contains supplementary material, which is available to authorized users.</P>

Phosphorylated Akt expression as a favorable prognostic factor for patients undergoing curative resection and adjuvant chemoradiotherapy for proximal extrahepatic bile duct cancer.

Kim, Kyubo,Min, Hye Sook,Chie, Eui Kyu,Jang, Jin-Young,Kim, Sun-Whe,Han, Sae-Won,Oh, Do-Youn,Im, Seock-Ah,Kim, Tae-You,Bang, Yung-Jue,Ha, Sung W. American Society of Clinical Oncology 2013 Journal of clinical oncology Vol.31 No.4

TGF-β Suppresses COX-2 Expression by Tristetraprolin-Mediated RNA Destabilization in A549 Human Lung Cancer Cells

Kang, Soyeong,Min, Ahrum,Im, Seock-Ah,Song, Sang-Hyun,Kim, Sang Gyun,Kim, Hyun-Ah,Kim, Hee-Jun,Oh, Do-Youn,Jong, Hyun-Soon,Kim, Tae-You,Bang, Yung-Jue Korean Cancer Association 2015 Cancer Research and Treatment Vol.47 No.1

<P><B>Purpose</B></P><P>Overexpression of cyclooxygenase 2 (COX-2) is thought to promote survival of transformed cells. Transforming growth factor β (TGF-β) exerts anti-proliferative effects on a broad range of epithelial cells. In the current study, we investigated whether TGF-β can regulate COX-2 expression in A549 human lung adenocarcinoma cells, which are TGF-β-responsive and overexpress COX-2.</P><P><B>Materials and Methods</B></P><P>Western blotting, Northern blotting, and mRNA stability assays were performed to demonstrate that COX-2 protein and mRNA expression were suppressed by TGF-β. We also evaluated the effects of tristetraprolin (TTP) on COX-2 mRNA using RNA interference.</P><P><B>Results</B></P><P>We demonstrated that COX-2 mRNA and protein expression were both significantly suppressed by TGF-β. An actinomycin D chase experiment demonstrated that COX-2 mRNA was more rapidly degraded in the presence of TGF-β, suggesting that TGF-β–induced inhibition of COX-2 expression is achieved via decreased mRNA stability. We also found that TGF-β rapidly and transiently induced the expression of TTP, a well-known mRNA destabilizing factor, before suppression of COX-2 mRNA expression was observed. Using RNA interference, we confirmed that increased TTP levels play a pivotal role in the destabilization of COX-2 mRNA by TGF-β. Furthermore, we showed that Smad3 is essential to TTP-dependent down-regulation of COX-2 expression in response to TGF-β.</P><P><B>Conclusion</B></P><P>The results of this study show that TGF-β down-regulated COX-2 expression via mRNA destabilization mediated by Smad3/TTP in A549 cells.</P>

Flat Burner 위에 설치된 Ceramic Honeycomb의 축열성능 연구

박재민(Jae-Min Park),허수빈(Su-Bin Heo),윤봉석(Bong-Seock Yoon),이도형(Do-Hyung Lee) 한국마린엔지니어링학회 2012 한국마린엔지니어링학회지 Vol.36 No.2

CD24 expression predicts distant metastasis in extrahepatic bile duct cancer.

Kim, Kyubo,Min, Hye Sook,Chie, Eui Kyu,Jang, Jin-Young,Kim, Sun Whe,Han, Sae-Won,Oh, Do-Youn,Im, Seock-Ah,Kim, Tae-You,Bang, Yung-Jue,Jang, Ja-June,Ha, Sung W WJG Press 2013 World journal of gastroenterology Vol.19 No.9

<P>To evaluate the prognostic significance of CD24 expression in patients undergoing adjuvant chemoradiotherapy for extrahepatic bile duct (EHBD) cancer.</P>

Phylogenetic relationships between three Korean pit viper Gloydius (Serpentes: Crotalinae) species using mitochondrial DNA genes

Lee Yun Sun,Do Min Seock,Kim Wanggyu,Jeon Hye Sook,Lee Sang-Cheol,Jung Ji-Hwa,An Junghwa 한국유전학회 2022 Genes & Genomics Vol.44 No.5

Background: Molecular phylogenetic studies of the Asian pit viper genus Gloydius have been widely published in Asia, but Korea population have not been conducted till date. Objective: This study aimed to analyze the phylogenetic relationships of three Gloydius species (G. saxatilis, G. brevicaudus, and G. ussuriensis) from Korea with other Gloydius species, based on Cytochrome b and ND4. Methods: We compared 160 samples representing the three species with those of 17 reference species and their phylogenetic status and genetic diversity were analyzed with concatenated sequences of two mitochondrial DNA. Results: Korean G. brevicaudus and G. saxatilis showed high haplotype diversity and relatively low and moderate nucleotide diversity, respectively. Although G. ussuriensis showed high genetic diversity, it was low in the Baengnyeong Island population. The phylogenetic tree represented two major lineages. One major lineage comprised G. ussuriensis, G. tsushimaensis, G. blomhoffii, and G. brevicaudus. The Chinese G. ussuriensis belonged to the same clade as the Korean G. ussuriensis and was closely related to the Baengnyeong Island population. Moreover, G. tsushimaensis was closely related to G. ussuriensis from southwestern Korean and Jeju Island populations. The other major lineage comprised the remaining 12 species and G. saxatilis. Korean G. saxatilis was closely related to G. saxatilis, G. shedanoensis, and G. intermedius from China. Conclusion: The phylogenetic status of the Korean Gloydius species in comparison with the other Gloydius species was identified. We suggesting the conservation management unit for the Baengnyeong Island population, while the current conservation status of Korean G. saxatilis is suggested to be revised to a higher level.