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      • KCI등재

        Duration Prediction Models for Construction Projects: In Terms of Cost or Physical Characteristics?

        Savas Bayram 대한토목학회 2017 KSCE Journal of Civil Engineering Vol.21 No.6

        The purpose of this study is to investigate and to validate the ‘Bromilow’s Time-cost (BTC) model’ and the ‘Love et al.’s Time- Floor (LTF) model’ for Turkish public building projects, to check their appropriateness and to compare their prediction performances on project duration. The LTF model formulates the project duration in terms of gross floor area & floor numbers while the BTC model formulated it in terms of cost. Despite the consideration of the different characteristics, both models were inspired from Australian building projects. The study also presents ‘best-fit models’ as a benchmark for the BTC and the LTF models in order to fill the gap in construction duration predictions. The research data were collected from a total of 530 Turkish public building projects which were completed in seven geographic regions by performing an archive search work. 80% of the total data (i.e. from 424 projects) were used to establish the models while the remaining 20% (106 projects) were used for validation. Eight project characteristics of; total construction area, building height, gross floor area, floor numbers, contract sum, actual cost, contract duration and the actual duration were collected. Since there was no central archiving conduction to obtain these characteristics, more than a thousand tender documents were examined, and for 530 documents these characteristics were available. Consequently, it was found that the BTC model is superior to the LTF model, which confirms that ‘cost’ is a more significant predictor of the ‘duration’ than ‘gross floor area’ & ‘floor numbers’. However, the proposed ‘best-fit model’ indicated that the ‘area’ and the ‘height’ are the main predictors of the ‘duration’ as well as the ‘cost’.

      • KCI등재

        The First Identified Citrus tristeza virus Isolate of Turkey Contains a Mixture of Mild and Severe Strains

        Bayram Çevik,Nejla Yard mc,Sava Korkmaz 한국식물병리학회 2013 Plant Pathology Journal Vol.29 No.1

        The presence of Citrus tristeza virus (CTV) has previously been reported in citrus growing regions of Turkey. All serologically and biologically characterized isolates including I d r, which was the first identified CTV isolates from Turkey, were considered mild isolates. In this study, molecular characteristics of the I d r isolate were determined by different methods. Analysis of the I d r isolate by western blot and BD-RT-PCR assays showed the presence of MCA13 epitope, predominantly found in severe isolates, in the I d r isolate revealing that it contains a severe component. For further characterization,the coat protein (CP) and the RNA-dependent RNA polymerase (RdRp) genes representing the 3'and 5' half of CTV genome, respectively, were amplified from dsRNA by RT-PCR. Both genes were cloned separately and two clones for each gene were sequenced. Comparisons of nucleotide and deduced amino acid sequences showed that while two CP gene sequences were identical, two RdRp clones showed only 90% and 91% sequence identity in their nucleotide and amino acid sequences, respectively, suggesting a mixed infection with different strains. Phylogenetic analyses of the CP and RdRp genes of I d r isolate with previously characterized CTV isolates from different citrus growing regions showed that the CP gene was clustered with NZRBTH30,a resistance breaking isolate from New Zealand,clearly showing the presence of severe component. Furthermore, two different clones of the RdRp gene were clustered separately with different CTV isolates with a diverse biological activity. While the RdRp-1 was clustered with T30 and T385, two well-characterized mild isolates from Florida and Spain, respectively, the RdRp-2 was most closely related to NZRB-G90 and NZRB-TH30, two well-characterized resistance breaking and stem pitting (SP) isolates from New Zealand confirming the mixed infection. These results clearly demonstrated that the I d r isolate, which was previously described as biologically a mild isolate, actually contains a mixture of mild and severe strains.

      • SCIEKCI등재

        The First Identified Citrus tristeza virus Isolate of Turkey Contains a Mixture of Mild and Severe Strains

        Cevik, Bayram,Yardimci, Nejla,Korkmaz, Sava The Korean Society of Plant Pathology 2013 Plant Pathology Journal Vol.29 No.1

        The presence of Citrus tristeza virus (CTV) has previously been reported in citrus growing regions of Turkey. All serologically and biologically characterized isolates including I$\breve{g}$d${\i}$r, which was the first identified CTV isolates from Turkey, were considered mild isolates. In this study, molecular characteristics of the I d r isolate were determined by different methods. Analysis of the I$\breve{g}$d${\i}$r isolate by western blot and BD-RT-PCR assays showed the presence of MCA13 epitope, predominantly found in severe isolates, in the I$\breve{g}$d${\i}$r isolate revealing that it contains a severe component. For further characterization, the coat protein (CP) and the RNA-depen-dent RNA polymerase (RdRp) genes representing the 3' and 5' half of CTV genome, respectively, were amplified from dsRNA by RT-PCR. Both genes were cloned separately and two clones for each gene were sequenced. Comparisons of nucleotide and deduced amino acid sequences showed that while two CP gene sequences were identical, two RdRp clones showed only 90% and 91% sequence identity in their nucleotide and amino acid sequences, respectively, suggesting a mixed infection with different strains. Phylogenetic analyses of the CP and RdRp genes of I$\breve{g}$d${\i}$r isolate with previously characterized CTV isolates from different citrus growing regions showed that the CP gene was clustered with NZRB-TH30, a resistance breaking isolate from New Zealand, clearly showing the presence of severe component. Furthermore, two different clones of the RdRp gene were clustered separately with different CTV isolates with a diverse biological activity. While the RdRp-1 was clustered with T30 and T385, two well-characterized mild isolates from Florida and Spain, respectively, the RdRp-2 was most closely related to NZRB-G90 and NZRB-TH30, two well-characterized resistance breaking and stem pitting (SP) isolates from New Zealand confirming the mixed infection. These results clearly demonstrated that the I$\breve{g}$d${\i}$r isolate, which was previously described as biologically a mild isolate, actually contains a mixture of mild and severe strains.

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