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RISS 인기검색어
- 검색키워드
- 저자 : Satyendra Nath Sharma (검색결과 3 건)
- 무료
- 기관 내 무료
- 유료
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Kumar, Vinod,Sharma, Satyendra Nath 한국작물학회 2011 Journal of crop science and biotechnology Vol.14 No.3
The assessment of genetic diversity is a primary concern of plant breeders and germplasm curators for which morphological traits are internationally accepted descriptors but are often associated with limitations due to G ${\times}$ E interactions. Phenotypic, ISSR, and SSR markers were employed to determine the genetic diversity and relationships among 20 commercially cultivated sesame genotypes representing different geographical regions of India. A narrow range of genetic dissimilarity (0.01 - 0.12) with mixed clustering was observed using morphological descriptors. A total 279 band positions with 57% polymorphism was amplified by ISSR primers, whereas 36 allelic positions with 73% polymorphism were produced by SSR primers with 100% reproducibility. The ISSR primers with high discrimination power were identified producing genotype-specific bands and distinguishing all the sesame genotypes used in this study. A poor correlation and lack of perfect congruence between phenotypes and marker-based analysis was indicated by the UPGMA dendrogram and PCA analysis. Our results suggest that SSR and ISSR markers provide a powerful tool for genotype identification and establishment of genetic relationships among sesame genotypes. We recommend the use of molecular markers for testing, required for obtaining intellectual property rights, and also for the analysis of genotypic diversity among sesame varieties.
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Vinod Kumar,Satyendra Nath Sharma 한국작물학회 2011 Journal of crop science and biotechnology Vol.14 No.3
The assessment of genetic diversity is a primary concern of plant breeders and germplasm curators for which morphological traits are internationally accepted descriptors but are often associated with limitations due to G x E interactions. Phenotypic, ISSR, and SSR markers were employed to determine the genetic diversity and relationships among 20 commercially cultivated sesame genotypes representing different geographical regions of India. A narrow range of genetic dissimilarity (0.01 - 0.12) with mixed clustering was observed using morphological descriptors. A total 279 band positions with 57% polymorphism was amplified by ISSR primers,whereas 36 allelic positions with 73% polymorphism were produced by SSR primers with 100% reproducibility. The ISSR primers with high discrimination power were identified producing genotype-specific bands and distinguishing all the sesame genotypes used in this study. A poor correlation and lack of perfect congruence between phenotypes and marker-based analysis was indicated by the UPGMA dendrogram and PCA analysis. Our results suggest that SSR and ISSR markers provide a powerful tool for genotype identification and establishment of genetic relationships among sesame genotypes. We recommend the use of molecular markers for testing,required for obtaining intellectual property rights, and also for the analysis of genotypic diversity among sesame varieties.
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Nidhi Shukla,Himani Kuntal,Asheesh Shanker,Satyendra Nath Sharma 한국작물학회 2018 Journal of crop science and biotechnology Vol.21 No.2
Seed priming is a commercially applied technique for improving seed vigor under variable field conditions. The present study was carried out to optimize the methods (direct: soaking in water 1:1 W/V and indirect: preconditioning under high RH ≥ 85%) and duration (hours)of hydro-priming, attempting to correlate the critical seed water content with priming-induced metabolic-restart in dry mungbean (Vigna radiataL.)seeds. Although the rate of water absorption and the amount of water absorbed was more by direct compared to the indirect imbibition method, the priming-induced germination responses were identical after 6hof priming treatment. The seed water content absorbed during this period was crucial for initiating the chain of biochemical events, required for early synchronized germination in primed seeds which was evident by improved membrane permeability, high activity of catalase and superoxide dismutase, and more integrated chloroplast and mitochondria in primed seeds. We demonstrate that the critical water content, required for priming-induced benefits for germination vigor, is a relative unit with respect to the duration of priming and the rate of water absorption. Seed priming is a co-ordinately regulated mechanism for controlling germination capacity of seeds by modifying the permeability characteristics of biological membranes and enzyme activity. This study helps to enhance our understanding on the potential of seed priming for synchronized germination and early seedling establishment in the field and beneficial for the resource poor farming community for better return of their limited financial/farm resources under variable environmental/field conditions.
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