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RISS 인기검색어
- 검색키워드
- 저자 : Sanghun Yeou (검색결과 3 건)
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Contribution of a DNA Nick to DNA Bendability Depending on the Bending Force
Sanghun Yeou,Nam Ki Lee 대한화학회 2021 Bulletin of the Korean Chemical Society Vol.42 No.8
A DNA nick plays a pivotal role in the bending and release of mechanical stress for double-stranded DNA (dsDNA). However, it remains unclear how a DNA nick modulates the bendability of dsDNA. Here, we report that a single nick in 30 base-pair dsDNA decreases the bending stiffness of dsDNA by 20%?30% and that the bendability of dsDNA depends on the applied bending force. To achieve this, we used D-shaped DNA nanostructures and single-molecule fluorescence resonance energy transfer for measurement of the bending angle of dsDNA. We found that a nick in dsDNA under a bending force increases the bendability of dsDNA and the bending stiffness of dsDNA decreases further as the applied bending force is increased. Our results provide a new perspective for the effect of a nick on dsDNA mechanics, and the methods introduced here will be useful for investigating various biological processes involving DNA nicks.
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Single-Molecule Methods for Investigating the Double-Stranded DNA Bendability
Sanghun Yeou,이남기 한국분자세포생물학회 2022 Molecules and cells Vol.45 No.1
The various DNA-protein interactions associated with the expression of genetic information involve double-stranded DNA (dsDNA) bending. Due to the importance of the formation of the dsDNA bending structure, dsDNA bending properties have long been investigated in the biophysics field. Conventionally, DNA bendability is characterized by innate averaging data from bulk experiments. The advent of single-molecule methods, such as atomic force microscopy, optical and magnetic tweezers, tethered particle motion, and single-molecule fluorescence resonance energy transfer measurement, has provided valuable tools to investigate not only the static structures but also the dynamic properties of bent dsDNA. Here, we reviewed the single-molecule methods that have been used for investigating dsDNA bendability and new findings related to dsDNA bending. Single-molecule approaches are promising tools for revealing the unknown properties of dsDNA related to its bending, particularly in cells.
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α-Synuclein Disrupts Vesicle Fusion by Two Mutant-Specific Mechanisms
Gyeongji Yoo,Hyeong Jeon An,Sanghun Yeou,Nam-Ki Lee 한국분자세포생물학회 2022 Molecules and cells Vol.45 No.11
Synaptic accumulation of α-synuclein (α-Syn) oligomers and their interactions with VAMP2 have been reported to be the basis of synaptic dysfunction in Parkinson’s disease (PD). α-Syn mutants associated with familial PD have also been known to be capable of interacting with VAMP2, but the exact mechanisms resulting from those interactions to eventual synaptic dysfunction are still unclear. Here, we investigate the effect of α-Syn mutant oligomers comprising A30P, E46K, and A53T on VAMP2-embedded vesicles. Specifically, A30P and A53T oligomers cluster vesicles in the presence of VAMP2, which is a shared mechanism with wild type α-Syn oligomers induced by dopamine. On the other hand, E46K oligomers reduce the membrane mobility of the planar bilayers, as revealed by single-particle tracking, and permeabilize the membranes in the presence of VAMP2. In the absence of VAMP2 interactions, E46K oligomers enlarge vesicles by fusing with one another. Our results clearly demonstrate that α-Syn mutant oligomers have aberrant effects on VAMP2-embedded vesicles and the disruption types are distinct depending on the mutant types. This work may provide one of the possible clues to explain the α-Syn mutant-type dependent pathological heterogeneity of familial PD.
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