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Ko, Yeong-Mu,Myung, Sung-Woon,Jung, Sang-Chul,Kim, Byung-Hoon American Scientific Publishers 2015 Journal of Nanoscience and Nanotechnology Vol.15 No.1
<P>In this study, titanium (Ti) surface was treated through the use of oxygen and nitrogen plasma for improving the initial adhesion and proliferation of preosteoblast cells. The plasma treated Ti surface demonstrated hydrophilic surface property and promoted initial adhesion and proliferation of preosteoblast cells.</P>
Application of Concanavalin A-Linked Magnetic Beads for the Detection of Hepatitis A Virus
KO, SANG-MU,CHO, SE-YOUNG,OH, MYUNG-JOO,KWON, JOSEPH,VAIDYA, BIPIN,KIM, DUWOON ational Association for Food Protection 2018 Journal of food protection Vol.81 No.12
<B>ABSTRACT</B><P>Prompt and inexpensive detection of hepatitis A virus (HAV) is essential to control acute hepatitis outbreaks associated with the consumption of contaminated raw or minimally processed food. In this study, various carbohydrate-binding lectins, including concanavalin A (Con A), wheat germ agglutinin, and soybean agglutinin, were compared for their binding affinity to HAV. Con A, which showed significantly higher binding affinity than other lectins, was used to develop an alternative and affordable method to conventional antibody-linked immunomagnetic separation prior to detection of HAV using reverse transcriptase PCR. This method, Con A-linked immunomagnetic separation combined with reverse transcriptase PCR, can detect HAV at a dilution concentration of 10−4 of the virus stock (titer: 104 median tissue culture infective dose per mL), indicating that Con A could be a promising candidate for concentrating HAV.</P>
KO, SANG-MU,VAIDYA, BIPIN,KWON, JOSEPH,LEE, HEE-MIN,OH, MYUNG-JOO,SHIN, TAI-SUN,CHO, SE-YOUNG,KIM, DUWOON International Association for Food Protection 2015 Journal of food protection Vol.78 No.5
<P>Outbreaks of hepatitis A virus (HAV) infections are most frequently associated with the consumption of contaminated oysters. A rapid and selective concentration method is necessary for the recovery of HAV from contaminated oysters prior to detection using PCR. In this study, ricin extracted from castor beans (Ricinus communis) was tested as an alternative to antibody used in immunomagnetic separation while concentrating HAV prior to its detection using reverse transcription PCR. Initially, the extracted proteins from castor beans were fractionated into 13 fractions by gel filtration chromatography. Pretreatment of different protein fractions showed a variation in binding of HAV viral protein (VP) 1 to oyster digestive tissue in the range of 25.9 to 63.9%. The protein fraction, which caused the highest reduction in binding of VP1 to the tissue, was identified as ricin A by quadrupole time-of-flight mass spectrometry. Ricin A could significantly inhibit binding of VP1 to the tissue with a 50% inhibitory concentration of 4.5 μg/ml and a maximal inhibitory concentration of 105.2%. The result showed that the rate of inhibition of HAV binding to tissue was higher compared to the rate of ricin itself binding to HAV (slope: 0.0029 versus 0.00059). However, ricin A concentration showed a higher correlation to the relative binding of ricin itself to HAV than the inhibition of binding of HAV to the tissue (coefficient of determination, R2: 0.9739 versus 0.6804). In conclusion, ricin A-linked magnetic bead separation combined with reverse transcription PCR can successfully detect HAV in artificially seeded oyster digestive tissue up to a 10−4 dilution of the virus stock (titer: 104 50% tissue culture infective dose per ml).</P>
Ko, Yeong-Mu,Myung, Sung-Woon,Kook, Joong-Ki,Jung, Sang-Chul,Kim, Byung-Hoon American Scientific Publishers 2015 Journal of Nanoscience and Nanotechnology Vol.15 No.1
<P>Antibacterial activity of oral pathogens such as Streptococcus mutans, Streptococcus sobrinus when silver ion immobilized on commercially pure (CP) titanium (Ti) surface was investigated in this study. Plasma-polymerized acrylic acid to have carboxyl group was deposited on CP-Ti surface and then ion-exchanged with Ag+ ions in 0.1 N AgNO3. In anti-adherent experiment, antibacterial activity was tested using broth culture methods. The biofilm formation assay was performed using semi-defined biofilm medium with sucrose. The silver coated CP-Ti completely inhibited the growth of S. mutans and S. sobrinus. In addition, the biofilm formation was significantly inhibited in silver-coated CP-Ti group.</P>
Ko, Yeong-Mu,Myung, Sung-Woon,Kim, Byung-Hoon American Scientific Publishers 2015 Journal of Nanoscience and Nanotechnology Vol.15 No.8
<P>In this study we performed O2/Ar plasma treatment to remove the polycaprolactone on hydroxyapatite nanopowder and polycaprolactone (HAp-NP/PCL) composite film. After plasma ashing, the HAp-NP was exposed on the composite film. The 25 wt% HAp-NP/PCL treated with plasma showed the hydrophilic surface property with reducing the aging effect. The MTT and ALP results indicated that the plasma etching increased the biocompatibility of HAp-NP/PCL composite film. The present simple plasma etching technique can be applicable in a development of biomaterials.</P>
Ko, Yeong-Mu,Choi, Do-Young,Jung, Sang-Chul,Kim, Byung-Hoon American Scientific Publishers 2015 Journal of Nanoscience and Nanotechnology Vol.15 No.1
<P>Polycaprolactone (PCL) nanofibers (PCL-NF) with uniform fibrous structure were fabricated by electrospinning. However, PCL-NF has hydrophobic surface, lacks functional groups and hence it is not a good substrate for cell adhesion. To improve the cell adhesion, PCL-NF surfaces were modified by low pressure RF discharge plasma treatment using monomer such as acrylic acid or oxygen gas. The plasma treated PCL-NFs improved the wettability and cell proliferation.</P>