Sequence Analysis of the Nuclear Matrix - Associated DNA Segments

Lee, Sang Eun,Park, Sang Dai,Lee, Chee Gun,Kim, Chan Kil 한국유전학회 1988 Genes & Genomics Vol.10 No.4

Nuclear matrix isolated from synchronized LP1-1 cells at G1/S boundary digested with DNase 1 to separate a nuclear-matrix(N.M)-associated DNA segments from the rest Through the cloning of the N.M-associated DNA segments (300-500 base pair in size) into a pUC18 vector, 800 clones (named as pMRs) were selected with X-Gal/IPTG and amphiciline. Putative clones harboring replication origin sequence were sequenced and characterized. We found that pMR15 and 16 act as a good template for origins of DNA replication in in vitro replication assay. Furthermore, pMR15 shows A/T richness and has a palindromic sequence. However, pMR62 shows G/C richness (65.7%) and has a homologous sequence to that of Adenovirus replication origin i.e. TGAPyGCC AGG.

A METRIC ON NORMED ALMOST LINEAR SPACES

Lee, Sang-Han,Jun, Kil-Woung Korean Mathematical Society 1999 대한수학회보 Vol.36 No.2

In this paper, we introduce a semi-metric on a normed almost linear space X via functional. And we prove that a normed almost linear space X is complete if and only if $V_X$ and $W_X$ are complete when X splits as X=$W_X$ + $V_X$. Also, we prove that the dual space $X^\ast$ of a normed almost linear space X is complete.

THE STABILITY OF THE EQUATION f(x+p) = kf(x)

Lee, Sang-Han,Jun, Kil-Woung Korean Mathematical Society 1998 대한수학회보 Vol.35 No.4

In this paper, we investigate the Hyers-Ulam stability of the (p,k)-MP functional equation.

SMEDDS (Seif-Micro Emulsifying Drug Delivery System) As An Intraurethral Prostaglandin E1 Delivery System

Sang Kil Lee,San Gok Jeon,Jae Seen Kang,Jae Hwi Lee,Young Wook Choi 韓國藥劑學會 2007 Journal of Pharmaceutical Investigation Vol.37 No.5

Antitumor Activity of 7-[2-(N-Isopropylamino)ethyl]-(20s)-camptothecin, CKD602, as a Potent DNA Topoisomerase I Inhibitor

Lee, Jun-Hee,Lee, Ju-Mong,Kim, Joon-Kyum,Ahn, Soon-Kil,Lee, Sang-Joon,Kim, Mie-Young,Jew, Sang-Sup,Park, Jae-Gab,Hong, Chung-Il The Pharmaceutical Society of Korea 1998 Archives of Pharmacal Research Vol.21 No.5

We developed a novel water-soluble camptothecin analobue, CKD602, and evaluated the inhibition of topoisomerase I and the antitumor activities against mammalian tumor cells and human tumor xenografts. CKD602 was a nanomolar inhibitor of the topoisomerase I enzyme in the cleavable complex assay. CKD602 was found to be 3 times and slightly more potent than topotecan and camptothecin as inhibitors of topoisomerase, respecitively. In tumor cell cytotoxicity, CKD602 was more potent than topotecan in 14 out of 26 human cancer cell lines tested, while it was comparable to camptothecin. CKD602 was tested for the in vivo antitumor activity against the human tumor xenograft models. CKD602 was able to imduce regression of established HT-29, WIDR and CX-1 colon tumors, LX-1 lung tumor, MX-1 breast tumor and SKOV-3 ovarian tumor as much as 80, 94, 76, 67, 87% and 88%, respectively, with comparable body weight changes to those of topotecan. Also the therapeutic margin (R/Emax: maximum tolerance dose/$ED-{58}$) of CKD602 was significantly higher than that of topotecan by 4 times. Efficacy was determined at the maximal tolerated dose levels using schedule dependent i.p. administration in mice bearing L1210 leukemia. On a Q4dx4 (every 4 day for 4 doses) schedule, the maximum tolerated dose (MTD) was 25 mg/kg per administration, which caused great weight loss and lethality in <5% tumor bearing mouse. this schedule brought significant increase in life span (ILS), 212%, with 33% of long-term survivals. The ex vivo antitumor activity of CKD602 was compared with that of topotecan and the mean antitumor index (ATI) values recorded for CKD602 were significantly higher than that noted for topotecan. From these results, CKD602 warrants further clinical investigations as a potent inhibitor of topoisomerase I.

Development of an Event Rainfall-Runoff Model in Small Watersheds

Lee, Sang-Ho,Lee, Kil-Seong Korea Water Resources Association 1995 Korean journal of hydrosciences Vol.6 No.-

A linear reservoir rainfall-runoff system was developed as a rainfall-runoff event simulation model. It was achieved from large modification of runoff function method. There are six parameters in the model. Hydrologic losses consist of some quantity of initial loss and some ratio of rainfall intensity followed by initial loss. The model has analytical routing equations. Hooke and Jaeves algorithm was used for model calibration. Parameters were estimated for flood events from '84 to '89 at Seomyeon and Munmak stream gauges, and the trends of major parameters were analyzed. Using the trends, verifications were performed for the flood event in September 1990. Because antecedent rainfalls affect initial loss, future researches are required on such effects. The estimation method of major parameters should also be studied for real-time forecasting.

Indoor Surveillance Camera based Human Centric Lighting Control for Smart Building Lighting Management

Sung Hoon Yoon,Kil Soo Lee,Jae Sang Cha,Vinayagam Mariappan,Min Woo Lee,Deok Gun Woo,Jeong Uk Kim 국제문화기술진흥원 2020 International Journal of Advanced Culture Technolo Vol.8 No.1

The human centric lighting (HCL) control is a major focus point of the smart lighting system design to provide energy efficient and people mood rhythmic motivation lighting in smart buildings. This paper proposes the HCL control using indoor surveillance camera to improve the human motivation and well-beings in the indoor environments like residential and industrial buildings. In this proposed approach, the indoor surveillance camera video streams are used to predict the day lights and occupancy, occupancy specific emotional features predictions using the advanced computer vision techniques, and this human centric features are transmitted to the smart building light management system. The smart building light management system connected with internet of things (IoT) featured lighting devices and controls the light illumination of the objective human specific lighting devices. The proposed concept experimental model implemented using RGB LED lighting devices connected with IoT features open-source controller in the network along with networked video surveillance solution. The experiment results are verified with custom made automatic lighting control demon application integrated with OpenCV framework based computer vision methods to predict the human centric features and based on the estimated features the lighting illumination level and colors are controlled automatically. The experiment results received from the demon system are analyzed and used for the real-time development of a lighting system control strategy.

Crystal Structure and Dielectric Responses of Pulsed Laser Deposited (Ba, Sr)$TiO_3$ Thin Films with Perovskite $LaNiO_3$ Metallic Oxide Electrode

Lee, Su-Jae,Kang, Kwang-Yong,Jung, Sang-Don,Kim, Jin-Woo,Han, Seok-Kil The Korean Ceramic Society 2000 The Korean journal of ceramics Vol.6 No.3

Highly (h00)-oriented (Ba, Sr)TiO$_3$(BST) thin films were grown by pulsed laser deposition on the perovskite LaNiO$_3$(LNO) metallic oxide layer as a bottom electrode. The LNO films were deposited on SiO$_2$/Si substrates by rf-magnetron sputtering method. The crystalline phases of the BST film were characterized by x-ray $\theta$-2$\theta$, $\omega$-rocking curve and $\psi$-scan diffraction measurements. The surface microsturcture observed by scanning electron microscopy was very dense and smooth. The low-frequency dielectric responses of the BST films grown at various substrate temperatures were measured as a function of frequency in the frequency range from 0.1 Hz to 10 MHz. The BST films have the dielectric constant of 265 at 1 kHz and showed multiple dielectric relaxation at the low frequency region. The origin of these low-frequency dielectric relaxation are attributed to the ionized space charge carriers such as the oxygen vacancies and defects in BST film, the interfacial polarization in the grain boundary region and the electrode polarization. We studied also on the capacitance-voltage characteristics of BST films.

Pituitary Apoplexy : Surgical Experience with 16 Patients

Lee, Jae-Hyun,Kim, Jae-Hyoo,Moon, Kyung-Sub,Joo, Sung-Pil,Lee, Jung-Kil,Kim, Soo-Han The Korean Neurosurgical Society 2007 Journal of Korean neurosurgical society Vol.42 No.2

Objective : Pituitary apoplexy, resulting from an acute infarction or hemorrhage mainly in pituitary adenomas, is a rare yet major clinical event with neurological, ophthalmological and hormonal emergent consequences. The authors review our surgical experience with a series of 16 cases of pituitary apoplexy. Methods : The cases of pituitary apoplexy, operated via trans-sphenoidal approach in our hospital between 1998-2005, were retrospectively analyzed in terms of their clinicoradiological features, pathological findings and surgical outcomes. Results : The mean age of patients (9 male and 7 female) was 47.1 years. The average time of presentation after onset of symptom was 8.9 days. Pituitary apoplexy occurred as an initial manifestation of pituitary adenoma in all patients. Headache was the most common presenting symptom (94%). Visual disturbance was found in 56% of patients. Thirty-one percent of the patients had hypopituitarism. On magnetic resonance imaging, this entire catastrophic event accompanied with macroadenoma in a mean size of 22.5 mm. Only four patients needed postoperative hormone replacement therapy. Three of them showed preoperative hypopituitary function, and one patient in normal pituitary function. There was no specific complication in any of these patients. Conclusion : Early trans-sphenoidal decompression with high-dose corticosteroid replacement showed good outcomes of pituitary apoplexy.

Iatrogenic Vertebral Artery Injury During Anterior Cervical Spine Surgery : Report of Two Cases

Lee, Jae-Hyun,Lee, Jung-Kil,Joo, Sung-Pil,Kim, Soo-Han The Korean Neurosurgical Society 2006 Journal of Korean neurosurgical society Vol.40 No.6

The incidence of vertebral artery injury during the anterior approach to the cervical spine is rare, but potentially lethal. The authors describe two cases of vertebral artery injury during anterior cervical decompression surgery. In the first case, infection was the cause of the vertebral artery injury. During aggressive irrigation and pus drainage, massive bleeding was encountered, and intraoperative direct packing with hemostatic agents provided effective control of hemorrhage. Ten days after surgery, sudden neck swelling and mental deterioration occurred because of rebleeding from a pseudoaneurysm. In the second case, the vertebral artery was injured during decompression of cervical spondylosis while drilling the neural foramen. After intraoperative control of bleeding, the patient was referred to our hospital, and a pseudoaneurysm was detected by angiography four days after surgery. Both pseudoaneurysms were successfully occluded by an endovascular technique without any neurological sequelae. Urgent vertebral angiography, following intraoperative control of bleeding by hemostatic compression in cases of vertebral artery injury during anterior cervical decompression, should be performed to avoid life-threatening complications. Prompt recognition of pseudoaneurysm is mandatory, and endovascular treatment can be life saving.