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Effect of Cationic and Anionic Porphyrins on the Structure and Activity of Adenosine Deaminase
Ajloo, Davood,Hajipour, Samaneh,Saboury, Ali Akbar,Zakavi, Saeed Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.9
Kinetic and structural studies have been carried out on the effects of meso-tetrakis(4-sulfonatophenyl)-porphyrin ($H_2TPPS_4$) as an anionic and meso-tetrakis(3-N-methyl-pyridyl)porphyrin ($H_2TMPYP$) as a cationic porphyrin with adenosine deaminase (ADA) in 25 mM citrate/phosphate buffer, pH = 4-8, at $37^{\circ}C$ using UVvis spectrophotometry, circular dichroism (CD), fluorescence spectrophotometry as well as molecular dynamics (MD) and molecular docking. Kinetic results showed that the two porphyrins are non-competitive inhibitors. Increasing pH, increases $K_I$ and cationic porphyrin has a higher $K_I$ and lower binding constant ($K_b$) at all pH ranges. Analyzing the secondary structure revealed that both ligands decrease the secondary structure and that the anionic porphyrin is more effective.
Effect of Cationic and Anionic Porphyrins on the Structure and Activity of Adenosine Deaminase
Davood Ajloo,Samaneh Hajipour,Ali Akbar Saboury,Saeed Zakavi 대한화학회 2011 Bulletin of the Korean Chemical Society Vol.32 No.9
Kinetic and structural studies have been carried out on the effects of meso-tetrakis(4-sulfonatophenyl)-porphyrin (H_2TPPS_4) as an anionic and meso-tetrakis(3-N-methyl-pyridyl)porphyrin (H_2TMPYP) as a cationic porphyrin with adenosine deaminase (ADA) in 25 mM citrate/phosphate buffer, pH = 4-8, at 37 ^oC using UVvis spectrophotometry, circular dichroism (CD), fluorescence spectrophotometry as well as molecular dynamics (MD) and molecular docking. Kinetic results showed that the two porphyrins are non-competitive inhibitors. Increasing pH, increases K_I and cationic porphyrin has a higher K_I and lower binding constant (K_b)at all pH ranges. Analyzing the secondary structure revealed that both ligands decrease the secondary structure and that the anionic porphyrin is more effective.