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Kazuhiro Ikeda,Yoshihisa Shimizu,Saburo Matsui,Takuya Oguri,Makoto Sekida,Atsusi Kawata,Taketoshi Kusakabe 대한토목학회 2008 KSCE Journal of Civil Engineering Vol.12 No.2
In this study, we investigated the partition property of pyrene onto cell membrane using synthetic membrane vesicles consisted ofseveral phospholipids and the effect of natural organic matters (NOM) on the partition. Membrane fluidity and membrane phaseaffected partition coefficient (Pmw) of pyrene onto the membrane. Pmw was larger for the liposome in liquid crystalline phase > inripple phase > in gel phase. Pyrene did not undergo the steric interfere in partition onto the membrane used in this study. Negativecharge of the membrane did not affect Pmw of pyrene. Suwannee River Natural Organic Matter (NOM) and Nordic fulvic acidaffected the partition of pyrene onto the TRANSILmembrane. The reason was confirmed that NOM associated pyrene did notpartition onto the membrane. Low molecular weigh fraction of SRNOM had strong effect on the partition as much as high molecularweigh fraction. From calorimetrical analysis, it was found that SRNOM did not affect the fluidity of the dimyristoy-lphosphatidylcholine (DMPC) membrane.