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        Immobilization of Fusarium graminearum β-D-fructofuranosidase Using Alternative Cellulosic Supports: Stabilization and Production of Fructooligosaccharides

        Heloísa Bressan Gonçalves,João Atílio Jorge,Luis Henrique Souza Guimarães 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.4

        The extracellular β-D-fructofuranosidase from Fusarium graminearum was immobilized using hydrophilic cotton, filter paper, multipurpose cloth, sugar cane bagasse, string, or gauze as alternative cellulosic supports, or with cyanogen bromide agarose. All derivatives (support+enzyme) showed high capacity for reuse (up to 23 times). The derivatives obtained with multipurpose cloth and string were stable at 60℃ maintaining 80% of their activity for more than 120 min. The filter paper derivative had a halflife (T50) of 27 min at 70℃. When tested for their pH stability (3.0-9.0), all derivatives were more stable than the free enzyme, especially the cotton derivative. The sugarcane bagasse, string, and filter paper derivatives could efficiently produce fructooligosaccharides (FOS) using sucrose as substrate. According to the retention of enzymatic activity, reuse and stabilities, the filter paper and string were the best alternative supports for β-D-fructofuranosidase immobilization, allowing enzyme stabilization and production of FOS.

      • KCI등재

        The effect of repeated bonding on the shear bond strength of different resin cements to enamel and dentin

        Ali Can Bulut,Saadet Sağlam Atsü 대한치과보철학회 2017 The Journal of Advanced Prosthodontics Vol.9 No.1

        PURPOSE. Cementation failures of restorations are frequently observed in clinical practice. The purpose of this study is to compare the effect of initial and repeated bonding on the bond strengths of different resin cements to enamel and dentin. MATERIALS AND METHODS. Ninety human maxillary central incisors were bisected longitudinally. The 180 tooth halves were divided into 2 groups (n = 90) for enamel and dentin bonding. The enamel and dentin groups were further divided into 3 groups (n = 30) for different resin cement types. Composite resin (Filtek Ultimate) cylinders (3 × 3 mm) were prepared and luted to enamel and dentin using Variolink II (Group V), RelyX ARC (Group R), or Panavia F 2.0 (Group P) resin cement. After 24 hours, initial shear bond strengths of the resin cements to enamel and dentin were measured. Using new cylinders, the specimens were de-bonded and re-bonded twice to measure the first and the second bond strengths to enamel and dentin. Failure modes and bonding interfaces were examined. Data were statistically analyzed. RESULTS. Initial and repeated bond strengths to enamel were similar for all the groups. The first (15.3 ± 2.2 MPa) and second (10.4 ± 2.2 MPa) bond strengths to dentin were significantly higher in Group V (P<.0001). Second bond strengths of dentin groups were significantly lower than initial and first bond strengths to dentin (P<.0001). CONCLUSION. All resin cements have similar initial and repeated bond strengths to enamel. Variolink II has the highest first and second bond strength to dentin. Bond strength to dentin decreases after the first re-bonding for all resin cements.

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        Determining Unemployment Factors Among Job Seeking Youth in the East Coast of Peninsular Malaysia

        SHAKUR, Elia Syarafina Abdul,SA'AT, Nor Hayati,AZIZ, Nazli,ABDULLAH, Siti Salina,RASID, Nor Hafzan Abd Korea Distribution Science Association 2020 The Journal of Asian Finance, Economics and Busine Vol.7 No.12

        This study examines factors of unemployment and career path challenges commonly faced by youth in the East Coast of Peninsular Malaysia. The study employed a quantitative approach which involved a total of 1,083 youths in Kelantan, Pahang and Terengganu selected at random. Descriptive and multiple regression analysis (OLS) methods were used to analyze demographic distribution of youth in determining factors influencing unemployment among them. The results reveal that the majority of youth respondents in the East Coast states of Malaysia are still unemployed and actively seeking employment. Demographic profiles are based on the survey results, youths seeking employment with 36 per cent distribution in Kelantan, 34 per cent in Pahang, and 30 per cent in Terengganu. Majority of the respondents were youths aged 20 to 24 years with a 40.9 percent percentage. The findings show that there are differences in factors affecting youth unemployment in the three states of East Coast, Malaysia. For Kelantan (age, experience, work mobility, marital status), Pahang (experience, marital status) and Terengganu (experience, gender, marital status, training). This study suggests that youths should be wise in preparing for their career by choosing the right career as well as doing side jobs to gain experience before they pursue for a real job.

      • KCI등재

        Return-on-Investment Measurement and Assessment of Research Fund: A Case Study in Malaysia

        Nur Azura SANUSI,Noor Hayati Akma SHAFIEE,Nor Ermawati HUSSAIN,Zuha Rosufila ABU HASAN,Mohd Lazim ABDULLAH,Nor Hayati SAAT 한국유통과학회 2021 The Journal of Asian Finance, Economics and Busine Vol.8 No.9

        This study estimates the financial value of return on investment (ROI) of research funds. Four simulation estimations are employed to measure ROI finance value that considers the outputs, outcomes, impacts and total ROI from the allocation input received. Research outputs, outcomes, and impacts can be quantitatively measured based on improvements to existing systems. In terms of input, the Malaysian government has allocated MYR301,350,000 for fundamental research in the 2021 budget compared with 2019, up 9.5 percent from 2019. It brings up the question: To what extent does the input of research funds allocated by the government yield a good return in outputs, outcomes, and impacts to the academic community, society, and country? The result of total ROI shows around MYR7 return is generated by researchers for each Malaysian ringgit channeled by the funder. More specifically, for a research project, it is more difficult to produce impacts and outcomes compared to research outputs. The positive return is evidence that all the allocated funds are beneficial to the stakeholders. The government can apply this approach in calculating ROI for evaluation and fund allocation to universities. Furthermore, the positive financial value of research output, outcome, and impact automatically contribute to a positive innovation environment in Malaysia.

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