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        Characterization of dihydrolipoamide dehydrogenase from the mitochondria of Helicoverpa armigera, a pest resistant to insecticides

        S. Md. AKBAR,S. K. JAYALAKSHMI,H. C. SHARMA,K. SREERAMULU 한국곤충학회 2011 Entomological Research Vol.41 No.6

        Dihydrolipoamide dehydrogenase (DHLDH) was isolated from the mitochondria of Helicoverpa armigera, a destructive pest which has developed resistance to commonly used insecticides. The flavoenzyme was purified 17.98-fold to homogeneity with an overall yield of 10.53% by employing ammonium sulfate precipitation, hydroxylapatite chromatography and CM-Sephadex chromatography. The purified enzyme exhibited the specific activity of 18.7 U/mg and was characterized as a dimer with a subunit mass of 66 kDa. The enzyme showed specificity for nicotinamide adenine dinucleotide – hydrogen (NADH) and lipoamide, as substrates, with Michaelis-Menten constants (K_m) of 0.083 mmol/L and 0.4 mmol/L, respectively. The reduction reaction of lipoamide by the enzyme could be explained by ping-pong mechanism. The spectra of DHLDH showed the maximum absorbance at 420 nm, 455 nm and 475 nm. The enzyme activity was strongly inhibited by mercurial and arsenical compounds. The N-terminal sequence of Ha-DHLDH showed homology with those of mammalian and arthropod DHLDH. Since H. armigera has developed high levels of resistance to commonly used insecticides, biochemical properties of the metabolic enzymes such as DHLDH, could be helpful to develop insecticidal molecules for the control of H. armigera, with a different mode of action.

      • Remote Controlling of Home Appliances using Mobile Telephony

        Rifat Shahriyar,Enamul Hoque,S.M. Sohan,Iftekhar Naim,Md. Mostafa Akbar,Masud Karim Khan 보안공학연구지원센터 2008 International Journal of Smart Home Vol.2 No.3

        In modern days, we must use various high-tech machineries and equipments to get our jobs done and make the life easier. These machineries should be controlled by the homeowner from any location as the homeowner might be away from home at workplace or traveling in a different place in the weekend. Thus a system of remote monitoring and controlling are very much necessary. Smart home is one of these types of system equipped with home appliances which we wish to control smartly from anywhere. Some products are commercially available which allow remote home appliance controlling through internet which is undoubtedly emerging. But it lacks the true sense of real mobility and security, making the remote home appliance controlling a limited term than it is supposed to be. In search of a true remote and adequately secure solution to be really effective and practicable, mobile telephony is better than any other solutions. Mobile phones have become almost an inseparable part of civil lives today. In this paper we introduce a new mechanism so that the ordinary services of the mobile phones can be leveraged to communicate with and control the home appliances and make our homes a really smart one.

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