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      저자 : Richard M. Dinsdale (검색결과 2 건)
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        The Effect of Physico-chemically Immobilized Methylene Blue and Neutral Red on the Anode of Microbial Fuel Cell

        Arseniy L. Popov,김중래,Richard M. Dinsdale,Sandra R. Esteves,Alan J. Guwy,Giuliano C. Premier 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.2

        A fast and cost effective immobilization of electron carriers, methylene blue (MB) and neutral red (NR) by pH shift was proposed to improve bioanodic performance. The adsorption of mediators onto the carbon cloth anode was verified using cyclic voltammogram (CV)and the effect of the immobilized mediators on acclimation,power density, and acetate removal of MFCs was investigated. A peak power density of Pmax(MB) = 11.3 W/m3was achieved over days 110 ~ 120, as compared to Pmax(Control) = 5.4 W/m3 and Pmax(NR) = 3.1 W/m3 for the treated anode after 15 sequential fed-batch operations. The VFA removal rates however were similar for all MFC systems, ranging from 82 to 87%. It could be suggested that the increase in power density for the MB treated electrode resulted from an enhanced electron transport from exo-electrogenic bacteria. MB may also have a selective effect on the bacterial community during the start-up stage, increasing the voltage production and acetate removal from day 1 to 16. However, MFC with NR treated anode produced an initial voltage under 100 mV, with lower coulombic efficiency (CE). NR exhibited less favourable mediator molecule binding to the electrode surface, when subject to pH driven physico-chemical immobilization.

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        Evaluation of Feeding Regimes to Enhance PHA Production Using Acetic and Butyric Acids by a Pure Culture of Cupriavidus necator

        Gopal Kedia,Pearl Passanha,Richard M. Dinsdale,Alan J. Guwy,Sandra R. Esteves 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.6

        In this study volatile fatty acids (VFAs), whichare by-products from anaerobic fermentations, have beenused as the carbon source for polyhydroxyalkanoates(PHA) production by pure culture bacteria, Cupriavidusnecator. A number of factors influence the conversionefficiencies of VFAs to PHAs including the bacterialfeeding regimes. When VFA was supplied as a single feed,it was found that concentrations higher than 2% v/v VFAled to substrate inhibition and only 18% acetic acid and12% of butyric acid was converted into PHA. This resultedin less than 65% (w/w) of PHA content within themicrobial cells. Hence, the single VFA feeding strategywas found to provide low conversion rates of VFA intopolymer. An improved feeding strategy was found to bethe use of an automatic VFA feed based on the pH controlof the medium, which led to a more continuous feedingregime. The conversion of VFA to PHA was increased byalmost 2-fold to 33 and 22% for acetic acid and butyricacid respectively, with up to 75% (w/w) of PHA resultantwithin the microbial cells.

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