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RISS 인기검색어
- 검색키워드
- 저자 : Rezaee Abbas (검색결과 4 건)
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Expression of Escherichia coli Heat-labile Enterotoxin B Subunit (LTB) in Saccharomyces cerevisiae
Rezaee Mohammad Ahangarzadeh,Rezaee Abbas,Moazzeni Seyed Mohammad,Salmanian Ali Hatef,Yasuda Yoko,Tochikubo Kunio,Pirayeh Shahin Najar,Arzanlou Mohsen The Microbiological Society of Korea 2005 The journal of microbiology Vol.43 No.4
Heat-labile enterotoxin B subunit (LTB) of enterotoxigenic Escherichia coli (ETEC) is both a strong mucosal adjuvant and immunogen. It is a subunit vaccine candidate to be used against ETEC-induced diarrhea. It has already been expressed in several bacterial and plant systems. In order to construct yeast expressing vector for the LTB protein, the eltB gene encoding LTB was amplified from a human origin enterotoxigenic E. coli DNA by PCR. The expression plasmid pLTB83 was constructed by inserting the eltB gene into the pYES2 shuttle vector immediately downstream of the GAL1 promoter. The recombinant vector was transformed into S. cerevisiae and was then induced by galactose. The LTB protein was detected in the total soluble protein of the yeast by SDS-PAGE analysis. Quantitative ELISA showed that the maximum amount of LTB protein expressed in the yeast was approximately $1.9\%$ of the total soluble protein. Immunoblotting analysis showed the yeast-derived LTB protein was antigenically indistinguishable from bacterial LTB protein. Since the whole-recombinant yeast has been introduced as a new vaccine formulation the expression of LTB in S. cerevisiae can offer an inexpensive yet effective strategy to protect against ETEC, especially in developing countries where it is needed most.
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Expression of Escherichia coli Heat-labile Enterotoxin B Subunit (LTB) in Saccharomyces cerevisiae
Mohammad Ahangarzadeh Rezaee,Abbas Rezaee,Seyed Mohammad Moazzeni,Ali Hatef Salmanian,Yoko Yasuda,Kunio Tochikubo,Shahin Najar Pirayeh,Mohsen Arzanlou 한국미생물학회 2005 The journal of microbiology Vol.43 No.4
Heat-labile enterotoxin B subunit (LTB) of enterotoxigenic Escherichia coli (ETEC) is both a strong mucosal adjuvant and immunogen. It is a subunit vaccine candidate to be used against ETEC-induced diarrhea. It has already been expressed in several bacterial and plant systems. In order to construct yeast expressing vector for the LTB protein, the eltB gene encoding LTB was amplified from a human origin enterotoxigenic E. coli DNA by PCR. The expression plasmid pLTB83 was constructed by inserting the eltB gene into the pYES2 shuttle vector immediately downstream of the GAL1 promoter. The recombinant vector was transformed into S. cerevisiae and was then induced by galactose. The LTB protein was detected in the total soluble protein of the yeast by SDS-PAGE analysis. Quantitative ELISA showed that the maximum amount of LTB protein expressed in the yeast was approximately 1.9% of the total soluble protein. Immunoblotting analysis showed the yeast-derived LTB protein was antigenically indistinguishable from bacterial LTB protein. Since the whole-recombinant yeast has been introduced as a new vaccine formulation the expression of LTB in S. cerevisiae can offer an inexpensive yet effective strategy to protect against ETEC, especially in developing countries where it is needed most.
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Robot Control Using Intelligent Gain Sliding Mode
Azita Yazdanpanah,Dr. Abbas ali Rezaee,Dr. Ahmad Faraahi 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.1
In this research, intelligent sliding mode controls are presented as robust controls for robot manipulators. The objective of the study is to design controls for robot manipulators without the knowledge of the boundary of the uncertainties by using an intelligent sliding mode control (SMC) while elucidating the robustness of the fuzzy SMC. A sliding mode control provides for unlimited accuracy in presence of bounded disturbance, although the sliding mode controller also causes chattering. Chattering is undesirable for use with actual component, since it might causes damage to them with a subsequent loss of accuracy. Such chatter is caused by overestimation of the controller gain. An intelligent sliding mode is proposed as a solution to the problems created by chattering; to illustrate, a continuum robot manipulator is simulated with an intelligent sliding mode control. The performance of intelligent gain sliding mode controller is demonstrated through the simulation results. The results of the simulations show the effectiveness for chattering mitigation by means of avoiding overestimation, and the robustness of an intelligent sliding mode control.
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Farideh Azimfar,Alireza Badiei,Seyed Mehdi Ghafelebashi,Majid Daftari-Besheli,Abbas Rezaee Shirin-Abadi 한국화학공학회 2018 Korean Journal of Chemical Engineering Vol.35 No.4
Three types of nanoporous silica support were modified by methylaluminoxane MAO and characterized by using BET, SEM, XRD and TGA. Dimethylsilyl (N-tert-butylamido)(tetramethylcyclopentadienyl) titanium dichlride was synthesized and immobilized on modified support. The prepared complex was then used as a reactive catalyst for ethylene polymerization. The effect of immobilization conditions on catalyst performance was studied. The results revealed elevated temperature grafting, decrease in precatalyst loading. Also, increasing of immobilization reaction time showed an increase in activity to 130 Kg poly/mol Ti.h.bar. The effects of polymerization temperature and [Al]/[Ti] ratio on the catalyst behavior, namely activity and bulk density, were investigated. According to the results, the activity of single-site catalyst depends on condition of immobilization and structure of nanoporous silica support.
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