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RISS 인기검색어
- 검색키워드
- 저자 : Reza Faramarzi Hafez (검색결과 4 건)
- 무료
- 기관 내 무료
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Reza Faramarzi Hafez,Bahram Heidari,Zeynab Shahabzadeh,Bahram. Heidari 한국작물학회 2013 Journal of crop science and biotechnology Vol.16 No.4
As a medicinal plant, the importance of evening primrose (Oenothera biennis L.) is due to its unsaturated fatty acids in the seedsand roots, and also oenotherine and comfarol in the leaves. Low germination and difficulties in seed production are the main problemsencountered with growing this plant in the field. As an alternative approach, an in vitro experiment was set up for the evaluationof evening primrose production via direct and indirect regeneration of the cultivars NC-1 and VNK. For callogenesis and directregeneration, the explants from the apical bud and petiole were cultured on MS medium supplemented with 0.25, 0.75, and 1.25 mgL-1 of both BAP and Kinetin (KIN). Indirect regeneration was performed by placing apical buds, petioles, and leaf explants on MSmedium supplemented with 0.5 and 1 mg L-1 2,4-D and 0.5, 1, and 1.25 mg L-1 of both BAP and KIN. The highest shoot inductionfrom direct regeneration was obtained with apical bud explants of VNK treated with 0.75 mg L-1 BAP. The highest callus weight(3.17 g) obtained from indirect regeneration was with petiole explants treated with 1 mg L-1 2, 4-D and 1 mg L-1 BAP in VNK cultivars. The highest number of torpedo embryogenic clusters (23.8) was obtained from the VNK petiole explants treated with 0.5 mg L-12, 4-D and 1.25 mg L-1 BAP. BAP had higher positive effects on in vitro production of evening primrose than KIN in both direct andindirect regeneration. In general, results indicated that VNK was more potent for regeneration than NC-1 and concentrations of 0.75mg L-1 BAP for direct and 0.5 mg L-1 2, 4-D and 1.25 mg L-1of BAP for indirect regeneration had a higher efficiency for increasing invitro production of evening primrose
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Hafez, Reza Faramarzi,Shahabzadeh, Zeynab,Heidari, Bahram,Ghadimzadeh, Morteza 한국작물학회 2013 Journal of crop science and biotechnology Vol.16 No.4
As a medicinal plant, the importance of evening primrose (Oenothera biennis L.) is due to its unsaturated fatty acids in the seeds and roots, and also oenotherine and comfarol in the leaves. Low germination and difficulties in seed production are the main problems encountered with growing this plant in the field. As an alternative approach, an in vitro experiment was set up for the evaluation of evening primrose production via direct and indirect regeneration of the cultivars NC-1 and VNK. For callogenesis and direct regeneration, the explants from the apical bud and petiole were cultured on MS medium supplemented with 0.25, 0.75, and $1.25mg\;L^{-1}$ of both BAP and Kinetin (KIN). Indirect regeneration was performed by placing apical buds, petioles, and leaf explants on MS medium supplemented with 0.5 and $1mg\;L^{-1}$ 2,4-D and 0.5, 1, and $1.25mg\;L^{-1}$ of both BAP and KIN. The highest shoot induction from direct regeneration was obtained with apical bud explants of VNK treated with $0.75mg\;L^{-1}$ BAP. The highest callus weight (3.17 g) obtained from indirect regeneration was with petiole explants treated with $1mg\;L^{-1}$ 2, 4-D and $1mg\;L^{-1}$ BAP in VNK cultivars. The highest number of torpedo embryogenic clusters (23.8) was obtained from the VNK petiole explants treated with $0.5mg\;L^{-1}$ 2, 4-D and $1.25mg\;L^{-1}$ BAP. BAP had higher positive effects on in vitro production of evening primrose than KIN in both direct and indirect regeneration. In general, results indicated that VNK was more potent for regeneration than NC-1 and concentrations of $0.75mg\;L^{-1}$ BAP for direct and $0.5mg\;L^{-1}$ 2, 4-D and $1.25mg\;L^{-1}$of BAP for indirect regeneration had a higher efficiency for increasing in vitro production of evening primrose.
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Zeynab Shahabzadeh,Bahram. Heidari,Reza Faramarzi Hafez 한국작물학회 2013 Journal of crop science and biotechnology Vol.16 No.4
An important feature of A. rhizogenes-induced hairy roots is their unique ability for investigation of gene function and productionof secondary metabolites such as diosgenin in fenugreek. In order to evaluate the transformation frequency and the efficiency oftransgenic hairy root induction, leaf and stem explants from two fenugreek ecotypes, Karaj and Bushehr, were infected with threeconcentrations of OD600= 0.8, 1.2, and 1.6 of A. rhizogenes strain K599 harboring a GFP gene. Regardless of ecotype, the ability ofstem explants for the induction of hairy roots (8.09) and the transformation frequency (81.3%) was higher compared with leafexplants with the values of 5.97 and 71.88%, respectively. The number of transgenic GFP-positive hairy roots ranged from 4.2 to13.5 in the Karaj ecotype and 3.8 to 9.9 in Bushehr. The highest transgenic hairy root (8.76), the transformation frequency (79.76%),and the growth rate of transgenic roots (0.77 d-1) were obtained from infection with K599 at OD600= 1.2, while the lowest belonged tothe bacterial concentration of OD600=1.6. Although the ecotype Bushehr had lower total roots (7.53) and transgenic hairy roots (6.08),it showed higher transformation frequency (79.56%) than Karaj (73.63%). Therefore, the results indicate the importance of genotype,type of explant and bacterial concentration in breeding for induction of transgenic hairy roots and consequently, production of secondarymetabolites in fenugreek.
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Shahabzadeh, Zeynab,Heidari, Bahram,Hafez, Reza Faramarzi 한국작물학회 2013 Journal of crop science and biotechnology Vol.16 No.4
An important feature of A. rhizogenes-induced hairy roots is their unique ability for investigation of gene function and production of secondary metabolites such as diosgenin in fenugreek. In order to evaluate the transformation frequency and the efficiency of transgenic hairy root induction, leaf and stem explants from two fenugreek ecotypes, Karaj and Bushehr, were infected with three concentrations of $OD_{600}=0.8$, 1.2, and 1.6 of A. rhizogenes strain K599 harboring a GFP gene. Regardless of ecotype, the ability of stem explants for the induction of hairy roots (8.09) and the transformation frequency (81.3%) was higher compared with leaf explants with the values of 5.97 and 71.88%, respectively. The number of transgenic GFP-positive hairy roots ranged from 4.2 to 13.5 in the Karaj ecotype and 3.8 to 9.9 in Bushehr. The highest transgenic hairy root (8.76), the transformation frequency (79.76%), and the growth rate of transgenic roots ($0.77d^{-1}$) were obtained from infection with K599 at $OD_{600}=1.2$, while the lowest belonged to the bacterial concentration of $OD_{600}=1.6$. Although the ecotype Bushehr had lower total roots (7.53) and transgenic hairy roots (6.08), it showed higher transformation frequency (79.56%) than Karaj (73.63%). Therefore, the results indicate the importance of genotype, type of explant and bacterial concentration in breeding for induction of transgenic hairy roots and consequently, production of secondary metabolites in fenugreek.
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