http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis
Alfredo García,Remigio Martínez,José Manuel Benitez-Medina,David Risco,Waldo Luis García,Joaquín Rey,Juan Manuel Alonso,Javier Hermoso de Mendoza 대한수의학회 2013 Journal of Veterinary Science Vol.14 No.4
Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus (D.) congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reaction. No cross-reaction with nucleic acids extracted from Pseudomonas aeruginosa,Mycobacterium tuberculosis, Staphylococcus aureus, or Austwickia chelonae was observed. Finally, the RT-PCR assay was used to evaluate clinical samples collected from naturally infected animals with D. congolensis. The results showed that this assay is a fast and reliable method for diagnosing dermatophilosis.