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- 저자 : Ranadhir Chakraborty (검색결과 2 건)
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Quorum sensing in metal tolerance of Acinetobacter junii BB1A is associated with biofilm production.
Sarkar, Suchitra,Chakraborty, Ranadhir Published by Elsevier/North Holland on behalf of t 2008 FEMS microbiology letters Vol.282 No.2
<P>Acinetobacter junii strain BB1A, a novel metal-tolerant bacterium, produced biofilm in the presence of added ions such as Ni(2+), AsO(2)(-), Cd(2+) and Hg(2+) on surfaces such as glass and polystyrene. Generation of a metal-sensitive and adhesion-deficient mutant by transposition of Tn5-mob in the A. junii genome has putatively confirmed the association of metal tolerance with the production of biofilm. The requirement of a critical cell density for biofilm formation and presence of acyl-homoserine lactone-like autoinducer molecules in the cell-free supernatant indicated the phenomenon of quorum sensing. Addition of a natural quorum-sensing inhibitor (garlic extract) or synthetic quorum-sensing inhibitor (4-nitro-pyridine oxide) significantly inhibited cell growth and biofilm formation in the presence of metal/metalloid ions.</P>
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Bipransh Kumar Tiwary,Anil Kumar,Ashis Kumar Nanda,Ranadhir Chakraborty 한국작물학회 2014 Journal of crop science and biotechnology Vol.17 No.3
The present study was conducted with the main objective to optimize petal yield from important marigold cultivars in WestBengal, and to standardize isolation of lutein from petals because these dietary xanthophylls are known to reduce the risks of agerelatedmacular degeneration (AMD) and cataracts. Six cultivars were studied viz. African marigold-Double (AFM-D), Africanmarigold-Single (AFM-S), African marigold-Orange (AFM-O), French marigold-Orange (FRM-O), French marigold-Double (FRMD),and LC (Local type), which withstand typical environmental conditions of northern West Bengal and produce flowers that do notvary in color and are relatively unaffected by pests and diseases. Lutein esters were extracted from milled marigold petals using nhexane. However, overall performance showed that LC is superior to the other cultivars. Pure lutein was obtained after saponificationwith 50% KOH. The re-crystallized lutein was characterized by UV-VIS, IR spectroscopy, and HPLC. A free-flowing lutein esterwas also formulated. This free-flowing lutein ester was found to be suitable to undergo commercialization or subsequent processing. An economic method for petal yield and isolation of lutein was thus standardized
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