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Isolation and Characterization of Novel Lipase Gene LipHim1 from the DNA Isolated from Soil Samples
Pavan Kumar Pindi,Raja Srinath R.,Theetha L. Pavankumar 한국미생물학회 2014 The journal of microbiology Vol.52 No.5
Metagenomics is a magnificent tool to isolate genes fromunknown/uncharacterized species and also from organismsthat cannot be cultured. In this study, we constructed a metagenomiclibrary from isolated DNA of soil samples collectedfrom Palamuru University campus premises, in Mahabubnagardistrict of Andhra Pradesh, India. We isolated a novellipase gene LipHim1, which has an open reading frame of591 base pairs and encodes ~23 kDa protein consisting of196 amino acids. The Lipase LipHim1 showed maximum32% homology at the protein level with the extracellularAeromonas hydrophila lipase (Class II, GDSL family) and wassignificantly different from all other known lipases. Theisolated lipase catalyzed the hydrolysis of fatty acid esters ofpolyoxyethylene sorbitan such as Tween 60. Our results indicatethat the isolated lipase gene is novel.