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Claudia Concha,María Daniella Carretta,Pablo Alarcón,Ivan Conejeros,Diego Gallardo,Alejandra Isabel Hidalgo,Nestor Tadich,Dante Daniel Cáceres,María Angélica Hidalgo,Rafael Agustín Burgos 대한수의학회 2014 Journal of Veterinary Science Vol.15 No.2
Reactive oxygen species (ROS) production is one of the mainmechanisms used to kill microbes during innate immuneresponse. D-lactic acid, which is augmented during acuteruminal acidosis, reduces platelet activating factor(PAF)-induced ROS production and L-selectin shedding inbovine neutrophils in vitro. This study was conducted toinvestigate whether acute ruminal acidosis induced by acuteoligofructose overload in heifers interferes with ROSproduction and L-selectin shedding in blood neutrophils. Blood neutrophils and plasma were obtained by jugularvenipuncture, while ruminal samples were collected usingrumenocentesis. Lactic acid from plasma and ruminal sampleswas measured by HPLC. PAF-induced ROS production andL-selectin shedding were measured in vitro in bovineneutrophils by a luminol chemiluminescence assay and flowcytometry, respectively. A significant increase in ruminal andplasma lactic acid was recorded in these animals. Specifically,a decrease in PAF-induced ROS production was observed 8 hafter oligofructose overload, and this was sustained until 48 hpost oligofructose overload. A reduction in PAF-inducedL-selectin shedding was observed at 16 h and 32 h postoligofructose overload. Overall, the results indicated thatneutrophil PAF responses were altered in heifers with ruminalacidosis, suggesting a potential dysfunction of the innateimmune response.