Ginsenoside-Rh2 Inhibits Proliferation and Induces Apoptosis of Human Gastric Cancer SGC-7901 Side Population Cells

Qian, Jun,Li, Jing,Jia, Jian-Guang,Jin, Xin,Yu, Da-Jun,Guo, Chen-Xu,Xie, Bo,Qian, Li-Yu Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.4

Objectives: To observed the effects of ginsenoside -Rh2 (GS-Rh2) on proliferation and apoptosis of side population (SP) human gastric cancer SGC-7901 cells. Materials and Methods: SGC-7901 SP and Non-SP cells were sorted by flow cytometry and assessed using the cck-8 method. Expression of apoptosis-related proteins Bax and Bcl-2 of SP before and after the intervention was determined by Western-blotting. Results: It was found that the proliferation of SP was significantly faster than that of NSP (P<0.05). In addition, GS-Rh2 inhibited proliferation of gastric cancer SP cells, induced cell cycle arrest and cell apoptosis, and changed the expression of BAX/Bcl-2 proteins in a time-dependent and concentration-dependent manner (P<0.05). Conclusions: With increase of GS-Rh2 dose, GS-Rh2 gradually inhibit the proliferation of SGC-7901 SP cells, which have high proliferation rate, through G1/G0 phase arrest, followed by apoptosis which involves the up-regulation of Bax and the down-regulation of Bcl-2.

Uniform Side Illumination Generated from LEDs Arranged by an Annealing Algorithm

Xu Wang,Panling Lei,Chaoyi Qian,Zhiping Wang,Xuefen Xu,Zhouping Su 한국광학회 2022 Current Optics and Photonics Vol.6 No.3

Given a cubic space, it is easy to uniformly illuminate the floor with light sources placed on top. However, little has been reported about uniform illumination on walls with the same configuration of light sources. Here we present a luminaire consisting of nine light-emitting diodes (LEDs) with perfect Lambertian distribution, placed on the top as a 3 × 3 rectangular LED array. The distances between LEDs and tilt angles of each individual LED are adjustable and optimized by an annealing algorithm. After optimization, the array produces a rectangular illumination pattern on one wall with a uniformity of about 89%. Analysis shows that the tilt angles of individual LEDs are key parameters for uniform side illumination. In a scenario that is more practical, the tilt angles of all the LEDs are set to be the same, only decreasing the uniformity to 83%.

Research on influence of harmonic wear wheel on wheel/rail contact geometry of high-speed train

Qian Xiao,Zhixiang Luo,Xu Xu,Jifeng Zheng,Shu Cheng 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.2

Harmonic wear is one of the common wear phenomena of high-speed train wheels. The change of wheel/rail rolling contact geometry leads to unstable characteristics of wheel/rail contact, which directly affects the quality and safety of high-speed train operation. Because wheelset lateral displacement directly affected the wheel/rail contact geometry, in this paper, the UM software was used to establish a high-speed train vehicle-track coupled dynamics simulation model of the CRH2 (China Railway High-speed 2) head car. Lateral displacement of harmonic wear wheel was calculated under different wear condition to analyze the influence of harmonic wear wheels on wheelset lateral displacement. Then according to macroscopic dimension change of the wheel profile from lateral and radial directions of high-speed train induced by harmonic wear wheels, wheel profiles under different wear condition were selected. And wheel/rail contact geometry under different harmonic wear stages was calculated to discuss the influence of harmonic wear wheel on wheel/rail contact geometry. The results show that harmonic order and wave depth of harmonic wear wheel have small influence on the lateral displacement of wheelset, but the influence of wave depth is greater than harmonic order. The average difference of lateral displacement between harmonic wear wheel and no harmonic wear wheel increases with the increase of wave depth and order. In a harmonic wave length, the maximum value of both equivalent conicity and contact angle appear in deepest wave depth. The greater the wave depth, the more obvious the fluctuations, and equivalent conicity changes are less obvious.

Hypoxia Mediates Runt-Related Transcription Factor 2 Expression via Induction of Vascular Endothelial Growth Factor in Periodontal Ligament Stem Cells

Xu, Qian,Liu, Zhihua,Guo, Ling,Liu, Rui,Li, Rulei,Chu, Xiang,Yang, Jiajia,Luo, Jia,Chen, Faming,Deng, Manjing Korean Society for Molecular and Cellular Biology 2019 Molecules and cells Vol.42 No.11

Periodontitis is characterized by the loss of periodontal tissues, especially alveolar bone. Common therapies cannot satisfactorily recover lost alveolar bone. Periodontal ligament stem cells (PDLSCs) possess the capacity of self-renewal and multilineage differentiation and are likely to recover lost alveolar bone. In addition, periodontitis is accompanied by hypoxia, and hypoxia-inducible $factor-1{\alpha}$ ($HIF-1{\alpha}$) is a master transcription factor in the response to hypoxia. Thus, we aimed to ascertain how hypoxia affects runt-related transcription factor 2 (RUNX2), a key osteogenic marker, in the osteogenesis of PDLSCs. In this study, we found that hypoxia enhanced the protein expression of $HIF-1{\alpha}$, vascular endothelial growth factor (VEGF), and RUNX2 ex vivo and in situ. VEGF is a target gene of $HIF-1{\alpha}$, and the increased expression of VEGF and RUNX2 proteins was enhanced by cobalt chloride ($CoCl_2$, $100{\mu}mol/L$), an agonist of $HIF-1{\alpha}$, and suppressed by 3-(5'-hydroxymethyl-2'-furyl)-1-benzyl indazole (YC-1, $10{\mu}mol/L$), an antagonist of $HIF-1{\alpha}$. In addition, VEGF could regulate the expression of RUNX2, as RUNX2 expression was enhanced by human VEGF ($hVEGF_{165}$) and suppressed by VEGF siRNA. In addition, knocking down VEGF could decrease the expression of osteogenesis-related genes, i.e., RUNX2, alkaline phosphatase (ALP), and type I collagen (COL1), and hypoxia could enhance the expression of ALP, COL1, and osteocalcin (OCN) in the early stage of osteogenesis of PDLSCs. Taken together, our results showed that hypoxia could mediate the expression of RUNX2 in PDLSCs via $HIF-1{\alpha}$-induced VEGF and play a positive role in the early stage of osteogenesis of PDLSCs.

Some Observations of the Influence Factors on the Response of Pile Groups

Qian-qing Zhang,Shi-min Zhang,Fayun Liang,Qian Zhang,Fei Xu 대한토목학회 2015 KSCE JOURNAL OF CIVIL ENGINEERING Vol.19 No.6

A simplified approach for nonlinear analysis of the load-displacement response of pile groups embedded in multilayered soils is presented in this work. A hyperbolic model is used to capture the relationship between unit skin friction and pile-soil relative displacement developed along the pile-soil interface and the stress-displacement relationship developed at the pile end. Considering interactive effect among piles, the parameters related to the hyperbolic model of an individual pile in a group can be computed. As to the analysis of the response of pile groups, a highly effective iterative computer program is developed using the hyperbolic model of an individual pile in a group. The efficiency and accuracy of the present method is verified using a well-documented field test. Furthermore, a parametric study is conducted to capture the influence of pile spacing and number of piles on the load-settlement response of the pile groups connected to a rigid cap. The pile-group settlement ratio and the pile-group resistance ratio are analyzed to assess the interaction effect among individual piles.

A Survey of Transfer and Multitask Learning in Bioinformatics

Xu, Qian,Yang, Qiang Korean Institute of Information Scientists and Eng 2011 Journal of Computing Science and Engineering Vol.5 No.3

Machine learning and data mining have found many applications in biological domains, where we look to build predictive models based on labeled training data. However, in practice, high quality labeled data is scarce, and to label new data incurs high costs. Transfer and multitask learning offer an attractive alternative, by allowing useful knowledge to be extracted and transferred from data in auxiliary domains helps counter the lack of data problem in the target domain. In this article, we survey recent advances in transfer and multitask learning for bioinformatics applications. In particular, we survey several key bioinformatics application areas, including sequence classification, gene expression data analysis, biological network reconstruction and biomedical applications.

An in-situ XPS and STM study on the thermal stability of Sm nanoparticles on the Al₂O₃ thin film substrate

Qian XU,Shanwei HU,Junfa ZHU 한국진공학회 2016 한국진공학회 학술발표회초록집 Vol.2016 No.8

Stigma and Efficacy of Zhizhu Kuanzhong Capsules Versus Doxepin in the Treatment of Refractory Functional Dyspepsia: A Randomized Controlled Trial

Qian-Qian Wang,Li Cheng,Bi-Yu Wu,Hong-Yi Qiu,Ping Xu,Bo Wang,Xiu-Juan Yan,Sheng-Liang Chen 대한소화기 기능성질환∙운동학회 2023 Journal of Neurogastroenterology and Motility (JNM Vol.29 No.3

Background/AimsStigma related with antidepressants is prevalent in patients with functional dyspepsia. It affects medication compliance and efficacy. Herbal medicine acquired a deep-rooted cultural identity in relieving dyspeptic symptoms in Asians. The research was designed to compare the effectiveness of Zhizhu Kuanzhong capsules (ZZKZ) versus doxepin hydrochloride (doxepin) on alleviating stigma and medication nonadherence among patients with refractory FD (rFD). MethodsPatients with rFD from February 2021 to February 2022 were randomly allocated to receive either doxepin (n = 56) or ZZKZ (n = 57) combined with omeprazole for 4 weeks. Medication possession ratio (MPR), the disease- and medication-associated stigma were analyzed. The scales were utilized to assess dyspeptic symptoms (Leeds Dyspepsia Questionnaire) and psychological conditions (Generalized Anxiety Disorder Questionnaire and Patient Health Questionnaire). ResultsThe MPR values for ZZKZ were significantly higher than those for doxepin (P < 0.001). The stigma scores decreased in ZZKZ group while increased in doxepin group compared to baseline after treatment. The proportion of patients showing ZZKZ-associated stigma was significantly lower than doxepin-associated stigma (P < 0.001). The MPR values were negatively correlated with post-treatment stigma scores in both groups (P < 0.001). Dyspeptic symptoms and psychological condition were improved in both groups after treatment, with no significant difference on post-treatment Leeds Dyspepsia Questionnaire, Generalized Anxiety Disorder Questionnaire, or Patient Health Questionnaire scores between 2 groups. ConclusionZZKZ is superior to doxepin in alleviating stigma and medication non-adherence, with comparable efficacy in improving dyspeptic symptoms and psychological condition of patients with rFD.

A Survey of Transfer and Multitask Learning in Bioinformatics

Qian Xu,Qiang Yang 한국정보과학회 2011 Journal of Computing Science and Engineering Vol.5 No.3

Machine learning and data mining have found many applications in biological domains, where we look to build predictive models based on labeled training data. However, in practice, high quality labeled data is scarce, and to label new data incurs high costs. Transfer and multitask learning offer an attractive alternative, by allowing useful knowledge to be extracted and transferred from data in auxiliary domains helps counter the lack of data problem in the target domain. In this article, we survey recent advances in transfer and multitask learning for bioinformatics applications. In particular, we survey several key bioinformatics application areas, including sequence classification, gene expression data analysis, biological network reconstruction and biomedical applications.

Lights, Camera, Music, Interaction! Interactive Persuasion in E-commerce

Xu, Qian,Sundar, S. Shyam SAGE Publications 2014 COMMUNICATION RESEARCH - Vol.41 No.2

<P>Heightened interactivity and excitement characterize much of our online browsing, especially when it involves shopping on e-commerce websites. Interactivity is said to affect users’ engagement with the website by expanding their perceptual bandwidth (Sundar, 2007), much like the effect of optimal physiological arousal on cognitive functioning (Kahneman, 1973). We examine the direct and combinatory effects of interactivity and arousal on consumers’ engagement, attitudes, and behavioral intentions in an e-commerce website through a 3 (interactivity: low, medium, high) × 3 (arousal: control, low, high) between-participants experiment (<I>N</I> = 186). Higher levels of interactivity were found to generate more favorable attitudes and behavioral intentions toward both the website and the product. Interactivity and arousal differed in their effects on various aspects of website engagement. The study also identified several mediators explicating the theoretical mechanism underlying the influence of interactivity on purchase likelihood.</P>