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Ponmani, T.,Guo, R.,Ki, J. S. Springer Science + Business Media 2016 Journal of applied phycology Vol.28 No.1
<P>Dinoflagellate algae are considered some of the most complicated organisms owing to their unusual genomic characteristics and novel gene regulatory mechanisms. Here, we extracted information from the genomic sequences of the marine dinoflagellate Prorocentrum minimum using GS FLX pyrosequencing technology. We obtained 473 Mb of sequences from 1,379,588 reads, which generated 16,599 contigs after assembly. Among the annotated sequences (4902 contigs, 30 %), BLAST analyses showed that 28.95 % (4731 contigs) of the genome fragments were homologous with bacterial sequences and only 0.96 % (156 contigs) had eukaryotic origins. However, analysis of bacterial 16S/23S rDNA sequences in the P. minimum genome revealed that the organism likely acquired this genetic material from symbiotic bacteria, possibly through horizontal gene transfer, thus demonstrating a definitive association between bacteria and P. minimum. Moreover, a specific consensus pattern was defined for dinoflagellate spliced leader (SL) sequences, a well-known genetic marker of dinoflagellates. Further, comparisons of the various genomic proportions with respect to nuclear, mitochondrial, and chloroplast genes revealed unique genomic features that include clusters of noncoding RNA genes and tandem repeats. Taken together, this study details the salient features of noncoding RNA genes in P. minimum and provides further insights into the genome of this and other dinoflagellates.</P>
Ponmani, K.,Kiruthika, S.,Muthukumaran, B. The Korean Electrochemical Society 2015 Journal of electrochemical science and technology Vol.6 No.3
In the present work, Carbon supported Pt<sub>100</sub>, Pt<sub>80</sub>Sn<sub>20</sub>, Pt<sub>80</sub>Ni<sub>20</sub> and Pt<sub>80</sub>Sn<sub>10</sub>Ni<sub>10</sub> electrocatalysts with different atomic ratios were prepared by ethylene glycol-reduction method to study the electro-oxidation of ethanol in membraneless fuel cell. The electrocatalysts were characterized in terms of structure, morphology and composition by using XRD, TEM and EDX techniques. Transmission electron microscopy measurements revealed a decrease in the mean particle size of the catalysts for the ternary compositions. The electrocatalytic activities of Pt<sub>100</sub>/C, Pt<sub>80</sub>Sn<sub>20</sub>/C, Pt<sub>80</sub>Ni<sub>20</sub>/C and Pt<sub>80</sub>Sn<sub>10</sub>Ni<sub>10</sub>/C catalysts for ethanol oxidation in an acid medium were investigated by cyclic voltammetry (CV) and chronoamperometry (CA). The electrochemical results showed that addition of Ni to Pt/C and Pt-Sn/C catalysts significantly shifted the onset of ethanol and CO oxidations toward lower potentials. The single membraneless ethanol fuel cell performances of the Pt<sub>80</sub>Sn<sub>10</sub>Ni<sub>10</sub>/C, Pt<sub>80</sub>Sn<sub>20</sub>/C and Pt<sub>80</sub>Ni<sub>20</sub>/C anode catalysts were evaluated at room temperature. Among the catalysts investigated, the power density obtained for Pt<sub>80</sub>Sn<sub>10</sub>Ni<sub>10</sub>/C (37.77 mW/cm<sup>2</sup> ) catalyst was higher than that of Pt<sub>80</sub>Sn<sub>20</sub>/C (22.89 mW/cm<sup>2</sup> ) and Pt<sub>80</sub>Ni<sub>20</sub>/C (16.77 mW/ cm<sup>2</sup> ), using 1.0 M ethanol + 0.5 M H<sub>2</sub>SO<sub>4</sub> as anode feed and 0.1 M sodium percarbonate + 0.5 M H<sub>2</sub>SO<sub>4</sub> as cathode feed.
Electrochemical Oxidation of Hydrazine in Membraneless Fuel Cells
Durga, S.,Ponmani, K.,Kiruthika, S.,Muthukumaran, B. The Korean Electrochemical Society 2014 Journal of electrochemical science and technology Vol.5 No.3
This paper describes the continuous flow operation of membraneless sodium perborate fuel cell using acid/alkaline bipolar electrolyte. Here, hydrazine is used as a fuel and sodium perborate is used as an oxidant under Alkaline-acid media configuration. Sodium perborate affords hydrogen peroxide in aqueous medium. In our operation, the laminar flow based microfluidic membranleless fuel cell achieved a maximum power density of $27.2mW\;cm^{-2}$ when using alkaline hydrazine as the anolyte and acidic perborate as the catholyte at room temperature with a fuel mixture flow rate of $0.3mL\;min^{-1}$. The simple planar structured membraneless sodium perborate fuel cell enables high design flexibility and easy integration of the microscale fuel cell into actual microfluidic systems and portable power applications.
Abassi, Sofia,Wang, Hui,Ponmani, Thangaraj,Ki, Jang‐,Seu John Wiley Sons, Inc. 2019 Environmental toxicology Vol.34 No.9
<P><B>Abstract</B></P><P>The freshwater green algae <I>Closterium ehrenbergii</I> has been considered as a model for eco‐toxicological assessment in aquatic systems. Heat shock proteins (HSPs) are a class of highly conserved proteins produced in all living organisms, which participate in environmental stress responses. In the present study, we determined the cDNA sequences of small heat shock protein 10 (<I>sHSP10</I>) and <I>sHSP17.1</I> from <I>C. ehrenbergii</I>, and examined the physiological changes and transcriptional responses of the genes after exposure to thermal shock and toxicants treatments. The open reading frame (ORF) of <I>CeHSP10</I> was 300 bp long, encoding 99 amino acid (aa) residues (10.53 kDa) with a GroES chaperonin conserved site of 22 aa. The <I>CeHSP17.1</I> had a 468 bp ORF, encoding 155 aa with a conserved C‐terminal α‐crystallin domain. For heat stress, cells presented pigment loss and possible chloroplast damage, with an up‐regulation in the expression of both <I>sHSP10</I> and <I>sHSP17.1</I> genes. As for the heavy metal stressors, an increase in the production of reactive oxygen species was registered in a dose dependent manner, with a significant up‐regulation of both <I>sHSP10</I> and <I>sHSP17.1</I> genes. These results suggest that <I>sHSP</I> genes in <I>C. ehrenbergii</I> may play a role in responses to stress environments, and they could be used as an early detection parameter as biomarker genes in molecular toxicity assessments.</P>