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Chhanda Debnath,Andrea Dobernig,Pijus Saha,Astrid Ortner 대한화학회 2011 대한화학회지 Vol.55 No.1
Sometimes inhabitants in remote areas have inadequate or no access to modern medicines or medical services. They can get benefit in term of the treatment against malaria by cultivating selected breeding of A. annua and making teas or decoctions from the plant materials following the proper way of tea preparation. In order to have the maximum extraction efficiency for artemisinin, different ways of tea preparations of A. annua were investigated by applying the developed DPP method and described in this article. Tea was prepared by three different ways (cooking, without cooking with/without shaking and microwave oven) with different times. From the results, it has been found that higher concentration of artemisinin (84.7%)can be attained by following the approach for tea preparation without cooking with shaking for 15 minutes (R.S.D. 2.34%). The concentration of artemisinin decreases with cooking more than 1.5 min in microwave oven. The utmost extraction (88.9%of artemisinin) is possible to extract by shaking with boiled 5% ethanol in distilled water (R.S.D. 2.28%).
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Debnath, Chhanda,Dobernig, Andrea,Saha, Pijus,Ortner, Astrid Korean Chemical Society 2011 대한화학회지 Vol.55 No.1
가끔 먼 지역 거주자들은 현대 의약품이나 의학 서비스에 있어서 불충분하거나 접근할 수 없다. 그들은 개똥쑥의 선택된 품종을 경작하고 차 제조의 적절한 방법에 따라 식물로부터 차나 달인즙을 만드는 것에 의해 말라리아에 대항한 치료의 관점에서 이익을 얻을 수 있다. 아르테미시닌에 대한 최대 추출 효율을 위해, 개똥쑥의 차제조의 다른 방법들은 발달된 DPP방법을 적용하여 연구되었고 이 논문에 서술되었다. 차는 시간을 다르게 하여 3가지 다른 방법으로 제조된다(굽기, 섞거나 섞지 않으면서 굽지 않기 그리고 마이크로 웨이브 오븐). 결과로부터, 아르테미시닌의 더 높은 농도(84.7%)는 15분 동안 섞으면서 굽지 않는 차 제조법에 의해 도달될 수 있다는 것을 발견했다(R.S.D. 2.34%). 아르테미시닌의 농도는 마이크로 웨이브 오븐에서 1.5분 이상 구울 때 감소한다. 최대한도의 추출(88.9%)은 증류수에서 5%에탄올과 함께 섞는 추출방법에서 가능했다(R.S.D. 2.28%). Sometimes inhabitants in remote areas have inadequate or no access to modern medicines or medical services. They can get benefit in term of the treatment against malaria by cultivating selected breeding of A. annua and making teas or decoctions from the plant materials following the proper way of tea preparation. In order to have the maximum extraction efficiency for artemisinin, different ways of tea preparations of A. annua were investigated by applying the developed DPP method and described in this article. Tea was prepared by three different ways (cooking, without cooking with/without shaking and microwave oven) with different times. From the results, it has been found that higher concentration of artemisinin (84.7%) can be attained by following the approach for tea preparation without cooking with shaking for 15 minutes (R.S.D. 2.34%). The concentration of artemisinin decreases with cooking more than 1.5 min in microwave oven. The utmost extraction (88.9% of artemisinin) is possible to extract by shaking with boiled 5% ethanol in distilled water (R.S.D. 2.28%).
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A. F. M. Mahmudul Islam,Md. Farhan Khalik,Nizam Uddin,Md. Sazzad Hossain,Md. Monir Hossain,Md. Mahadi Hasan,S. M. Fahad,Pijus Saha 한국약제학회 2015 Journal of Pharmaceutical Investigation Vol.45 No.2
The aim of this study was to scrutinize thephysicochemical and qualitative microbial examination of 30 different (of various dosage forms) herbal medicinalproducts marketed by pharmaceutical and herbal manufacturersin Bangladesh. The microbial evaluation includedtotal viable aerobic bacteria, Escherichia coli, fungi andStaphylococcus aureus count; physicochemical propertieslike weight variation, hardness, disintegration time, friabilityand density. The IMViC (indole, methyl red, Voges–Proskauer and citrate utilization) tests were frequentlyemployed for identification of E. coli and S. aureus. Totalviable aerobic bacterial counts in the samples analyzed wereranged from 3.8 × 10⁴ to 3.2 × 108 CFU/ml or CFU/g and 36.67 % were within BP standard limit. S. aureus wasdetected in 53.33 % (16) of the samples, ranged from anestimated 3 × 10² to 9.2 × 106 CFU/ml or CFU/g whichwere above the USP standard. E. coli was detected in 40 %(12) of the samples, ranging from 1 × 10² to 4.8 × 10⁴CFU/ml or CFU/g that were above the USP standard. Theranges of the fungi counts were 3.4 × 10³ – 3.1 × 106 CFU/ml or CFU/g and 46.67 %of which were within BP standard. Physicochemical properties of herbal products manufacturedby pharmaceutical manufacturers were found betterthan that of the herbal manufacturers. From the label andpack insert of herbal products, definite release patterninformation weren’t obtained and the majority of herbalproducts showed scattered disintegration time. In order toreduce the potential health related complications, there isnecessity of constant monitoring and control of the standardsof herbal medicine products available in the Bangladeshimarket.
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