http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Field measurement and CFD simulation of wind pressures on rectangular attic
Yongbo Peng,Weijie Zhao,Xiaoqiu Ai 한국풍공학회 2019 Wind and Structures, An International Journal (WAS Vol.29 No.6
Wind pressure is a critical argument for the wind-resistant design of structures. The attempt, however, to explore the wind pressure field on buildings still encounters challenges though a large body of researches utilizing wind tunnel tests and wind field simulations were carried out, due to the difficulty in logical treatments on the scale effect and the modeling error. The full-scale measurement has not yet received sufficient attention. By performing a field measurement, the present paper systematically addresses wind pressures on the rectangular attic of a double-tower building. The spatial and temporal correlations among wind speed and wind pressures at measured points are discussed. In order to better understand the wind pressure distribution on the attic facades and its relationship against the approaching flow, a full-scale CFD simulation on the similar rectangular attic is conducted as well. Comparative studies between wind pressure coefficients and those provided in wind-load codes are carried out. It is revealed that in the case of wind attack angle being zero, the wind pressure coefficient of the cross-wind facades exposes remarkable variations along both horizontal and vertical directions; while the wind pressure coefficient of the windward facade remains stable along horizontal direction but exposes remarkable variations along vertical direction. The pattern of wind pressure coefficients, however, is not properly described in the existing wind-load codes.
Comparative Study of Two Common In Vitro Models for the Pancreatic Islet with MIN6
Chao Xinxin,Zhao Furong,Hu Jiawei,Yu Yanrong,Xie Renjian,Zhong Jianing,Huang Miao,Zeng Tai,Yang Hui,Luo Dan,Peng Weijie 한국조직공학과 재생의학회 2023 조직공학과 재생의학 Vol.20 No.1
BACKGROUND: Islet transplantation is currently considered the most promising method for treating insulin-dependent diabetes. The two most-studied artificial islets are alginate-encapsulated b cells or b cell spheroids. As three-dimensional (3D) models, both artificial islets have better insulin secretory functions and transplantation efficiencies than cells in twodimensional (2D) monolayer culture. However, the effects of these two methods have not been compared yet. Therefore, in this study, cells from the mouse islet b cell line Min6 were constructed as scaffold-free spheroids or alginate-encapsulated dispersed cells. METHODS: MIN6 cell spheroids were prepared by using Agarose-base microwell arrays. The insulin secretion level was determined by mouse insulin ELISA kit, and the gene and protein expression status of the MIN6 were performed by Quantitative polymerase chain reaction and immunoblot, respectively. RESULTS: Both 3D cultures effectively promoted the proliferation and glucose-stimulated insulin release (GSIS) of MIN6 cells compared to 2D adherent cells. Furthermore, 1% alginate-encapsulated MIN6 cells demonstrated more significant effects than the spheroids. In general, three pancreatic genes were expressed at higher levels in response to the 3D culture than to the 2D culture, and pancreatic/duodenal homeobox-1 (PDX1) expression was higher in the cells encapsulated in 1% alginate than that in the spheroids. A western blot analysis showed that 1% alginate-encapsulated MIN6 cells activated the phosphoinositide 3-kinase (PI3K)/serine/threonine protein kinase (AKT)/forkhead transcription factor FKHR (FoxO1) pathway more than the spheroids, 0.5% alginate-, or 2% alginate-encapsulated cells did. The 3D MIN6 culture, therefore, showed improved effects compared to the 2D culture, and the 1% alginate-encapsulated MIN6 cells exhibited better effects than the spheroids. The upregulation of PDX1 expression through the activation of the PI3K/AKT/FoxO1 pathway may mediate the improved cell proliferation and GSIS in 1% alginate-encapsulated MIN6 cells. CONCLUSION: This study may contribute to the construction of in vitro culture systems for pancreatic islets to meet clinical requirements.