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      • Hepatic Angiomyolipoma: Contrast Patterns with SonoVue-enhanced Real-time Gray-scale Ultrasonography

        Wei, Rui-Xue,Wang, Wen-Ping,Ding, Hong,Huang, Bei-Jian,Li, Chao-Lun,Fan, Pei-Li,Hou, Jun,He, Nian-An Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.2

        This study was conducted to retrospectively evaluate the pattern of contrast enhancement with SonoVue on gray-scale ultrasonography of hepatic angiomyolipoma (HAML). Imaging features of 33 pathologically proven HAML lesions in 33 patients who underwent baseline ultrasound and contrast-enhanced ultrasonography (CEUS) were assessed retrospectively. All lesions were enhanced in the arterial phase and showed whole-tumor filling in. Thirty-two of 33 (97%) lesions showed early positive enhancement in the arterial phase. Twenty-three of these exhibited isoechoic or hyperechoic features in the portal phase. HAML demonstrate characteristic manifestations with SonoVue-enhanced real-time gray-scale ultrasonography.

      • SCIESCOPUSKCI등재

        Construction of Mammalian Cell Expression Vector for pAcGFP-bFLIP(L) Fusion Protein and Its Expression in Follicular Granulosa Cells

        Yang, Run Jun,Li, Wu Feng,Li, Jun Ya,Zhang, Lu Pei,Gao, Xue,Chen, Jin Bao,Xu, Shang Zhong Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.3

        FLICE inhibitory protein (FLIP) is one of the important anti-apoptotic proteins in the Fas/FasL apoptotic path which has death effect domains, mimicking the pro-domain of procaspase-8. To reveal the intracellular signal transduction molecules involved in the process of follicular development in the bovine ovary, we cloned the c-FLIP(L) gene in bovine ovary tissue with the reverse transcription polymerase chain reaction (RT-PCR), deleted the termination codon in its cDNA, and directionally cloned the amplified c-FLIP(L) gene into eukaryotic expression vector pAcGFP-Nl, including AcGFP, and successfully constructed the fusion protein recombinant plasmid. After identifying by restrictive enzyme BglII/EcoRI and sequencing, pAcGFP-bFLIP(L) was then transfected into follicular granulosa cells, mediated by Lipofectamine 2000, the expression of AcGFP observed and the transcription and expression of c-FLIP(L) detected by RT-PCR and Western blot. The results showed that the cattle c-FLIP(L) was successfully cloned; the pAcGFPbFLIP(L) fusion protein recombinant plasmid was successfuly constructed by introducing a BglII/EcoRI cloning site at the two ends of the c-FLIP(L) open reading frame and inserting a Kozak sequence before the start codon. AcGFP expression was detected as early as 24 h after transfection. The percentage of AcGFP positive cells reached about 65% after 24 h. A 1,483 bp transcription was amplified by RT-PCR, and a 83 kD target protein was detected by Western blot. Construction of the pAcGFP-bFLIP(L) recombinant plasmid should be helpful for further understanding the mechanism of regulation of c-FLIP(L) on bovine oocyte formation and development.

      • The Loss Calculation of RCD Snubber with Forward and Reverse Recovery Effects Considerations

        Chen Cai,Pei Xue-jun,Chen Yu,Kang Yong 전력전자학회 2011 ICPE(ISPE)논문집 Vol.2011 No.5

        This paper addresses the problem of turn-off and turn-on performances of a RCD snubber circuit in high power inverter that works with insulated gate bipolar transistor (IGBT). The RCD snubber circuit turn-off and turn-on dynamics, forward and reverse recovery effects of the associated freewheeling and snubber diodes, and corresponding snubber losses are analyzed. Voltage impacts occur across the snubber resistor due to the snubber diode’s forward and reverse recovery effects, which bring in additional snubber losses. The turn-on current rising rates have two parts due to the anti-parallel free-wheeling diode (FWD) reverse recovery current that will influence the RCD loss. Moreover, the snubber has the same clamping effect as turn-off due to the anti-parallel FWD’s reverse recovery current. In addition, the proposed analysis was experimentally verified, the snubber loss are presented and discussed in the paper. Moreover, a well-designed temperature measurement is proposed to evaluate the loss.

      • SCIESCOPUSKCI등재

        Association of CAST Gene Polymorphisms with Carcass and Meat Quality Traits in Chinese Commercial Cattle Herds

        Li, Jiao,Zhang, Lu-Pei,Gan, Qian-Fu,Li, Jun-Ya,Gao, Hui-Jiang,Yuan, Zheng-Rong,Gao, Xue,Chen, Jin-Bao,Xu, Shang-Zhong Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.11

        Calpastatin (CAST), an endogenous inhibitor of the calpains, plays an important role in post-mortem tenderization of meat. The objectives of this study were to investigate single nucleotide polymorphisms (SNPs) in the bovine CAST gene and association with carcass and meat quality traits. A total of 212 cattle from commercial herds were tested in this study including 2 pure introduced breeds, 4 cross populations, and 3 pure Chinese native breeds. Five SNPs were identified at position 2959 (A/G), 2870 (G/A), 3088 (C/T), 3029 (G/A) and 2857 (C/T) in the CAST gene (GenBank Accession No. AF159246). Allele frequencies of SNP2959 and SNP2870 were 0.701 (A) and 0.462 (A), respectively. A general linear model was used to evaluate the associations between the two markers and 7 traits. The results showed that both SNP2959 and SNP2870 were significantly (p<0.01) associated with the Warner-Bratzler shear force (WBSF), while they had no significant association with the other 6 traits in the whole population. However, in Chinese native pure breeds, only SNP2870 had significant association with WBSF (p<0.05). The simultaneous analysis of two-marker genotype effects indicated animals containing the A/G haplotype (A for SNP2959 and G for SNP2870) tended to have lower shear force than those containing the G/A haplotype, and, especially, animals homozygous for the A/G haplotype had approximately 2 kg lower shear force than those homozygous for the G/A haplotype (p<0.01). These results suggested that both markers may be effective for the marker-assisted selection of meat quality traits in Chinese commercial herds, especially SNP2870 which can be used for Chinese native cattle.

      • Analysis of Mammographic Breast Density in a Group of Screening Chinese Women and Breast Cancer Patients

        Liu, Jing,Liu, Pei-Fang,Li, Jun-Nan,Qing, Chun,Ji, Yu,Hao, Xi-Shan,Zhang, Xue-Ning Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15

        Background: A dense breast not only reduces the sensitivity of mammography but also is a moderate independent risk factor for breast cancer. The percentage of Western women with fat breast tissue is higher aged 40 years or older. To a certain extent, mammography as a first choice of screening imaging method for Western women of this group is reasonable. Hitherto, the frequency and age distribution of mammographic breast density patterns among Chinese women had not been characterized. The purpose of this study was to investigate the frequency and age distribution of mammographic breast density patterns among a group of Chinese screening women and breast cancer patients in order to provide useful information for age-specific guidelines for breast cancer screening in Chinese women. Methods: A retrospective review of a total of 3,394 screening women between August and December 2009 and 2,527 breast cancer patients between July 2011 and June 2012 was conducted. Descriptive analyses were used to examine the association between age and breast density. The significance of differences of breast density between the screening women and the breast cancer patients was examined using nonparametric tests. Results: There was a significant inverse relationship between age and breast density overall (r=-0.37, p< 0.01). Breast density of the breast cancer patients in the subgroups of 40-49 years old was greater compared with that of the screening women, the same in those aged 50-54 years and in those 55 years old or older, less than in the screening group. Conclusions: With regard to the Chinese women younger than 55 years old, the diagnostic efficiency of breast cancer screening imaging examinations may be potentially improved by combining screening mammography with ultrasound.

      • KCI등재

        Sonication-Assisted Synthesis of Molybdenum Disulfide Aerogel for the Electrode Materials of Supercapacitors

        Qiang Zhang,Wenyuan He,Yinmin Wang,Dazhao Pei,Xue Jun Zheng 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.5

        The sonication processing was added in front of the freeze-drying as an intermediate processing before the molybdenum disulfide (MoS2) aerogel was synthesized. It is distinguishing with the traditional hydrothermal reaction to combine the sonication processing and freeze-drying in our method. The structure, morphology, specific surface area and pore size distribution were characterized, and the electrochemical performances were measured in 0.5M Na2SO4 electrolyte for the MoS2 aerogel and flower-like MoS2. As for comparison, they are of porous structure and microsphere structure, and their specific surface areas are 55.14 m2 g -1 and 38.12 m2 g -1. The specific capacitances are 166.7 F g -1 and 119.2 F g -1 at the scan rate of 5 mV s -1, and the capacity retentions are 87.7% and 81.6% after 3000 charge/discharge cycles. For the enhanced mechanism, the high specific surface of the MoS2 aerogel causes high specific capacitance, and the unique porous structure could buffer volume expansion to improve retention ability during charge/discharge processes. The MoS2 aerogel may thus be a promising electrode material for supercapacitors.

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