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        Compositional bias coupled with selection and mutation pressure drives codon usage in Brassica campestris genes

        Paul, Prosenjit,Malakar, Arup Kumar,Chakraborty, Supriyo 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.3

        The plant Brassica campestris includes the vegetables turnip and Chinese cabbage, important plants of economic importance. Here, we have analysed the codon usage bias of B. campestris for 116 protein coding genes. Neutrality analysis showed that B. campestris had a wide range of GC3s, and a significant correlation was observed between GC12 and GC3. Nc versus GC3s plot showed a few genes on or proximate to the expected curve, but the majority of points were found to be scattered distantly from the expected curve. Correspondence analysis on codon usage revealed that the position preference of codons on multidimensional space totally depends on the presence of A and T at synonymous third codon position. These results altogether suggest that composition bias along with selection (major) and mutation pressure (minor) affects the codon usage pattern of the protein coding genes in Brassica campestris.

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        Compositional bias coupled with selection and mutation pressure drives codon usage in Brassica campestris genes

        Prosenjit Paul,Arup Kumar Malakar,Supriyo Chakraborty 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.3

        The plant Brassica campestris includes the vegetables turnip and Chinese cabbage, important plants of economic importance. Here, we have analysed the codon usage bias of B. campestris for 116 protein coding genes. Neutrality analysis showed that B. campestris had a wide range of GC3s, and a significant correlation was observed between GC12 and GC3. Nc versus GC3s plot showed a few genes on or proximate to the expected curve, but the majority of points were found to be scattered distantly from the expected curve. Correspondence analysis on codon usage revealed that the position preference of codons on multidimensional space totally depends on the presence of A and T at synonymous third codon position. These results altogether suggest that composition bias along with selection (major) and mutation pressure (minor) affects the codon usage pattern of the protein coding genes in Brassica campestris.

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        Gene clustering and co-expression analysis for the identification of putative transcription factors associated with the genes of secondary metabolic pathways in Plantago ovata Forsk. and its wild allies

        Gupta Suruchi,Singh Ravail,Paul Prosenjit,Kaul Sanjana,Lattoo Surrinder K.,Dhar Manoj K. 한국식물생명공학회 2024 Plant biotechnology reports Vol.18 No.1

        Genus Plantago of Plantaginaceae family is bestowed with a repertoire of structurally diverse secondary metabolites that have not only been used as therapeutics but also effect the plant physiology by conferring adaptive advantages under stress. Assuming that domestication process in plants has influenced their secondary metabolites, we performed a comparative transcriptome of wild and cultivated species of Plantago to analyze the variation in the expression of genes related to sec- ondary metabolite pathways. GO and KEGG analysis of DEGs in wild species showed their enrichment in abiotic stresses, oxidation–reduction and secondary metabolite related pathways. Overall, we found upregulation of genes of carotenoid, flavonoid and phenylpropanoid, isoprenoid, terpenoid and alkaloid pathways in wild species of Plantago in particular, P. lanceolata while mucilage pathway-related genes showed higher expression in P. ovata. Moreover, transcriptome data pre- sented putative transcription factors associated with terpenoids, carotenoid and phenylpropanoid biosynthetic pathways which were also identified using co-expression analysis of cluster 9 (secondary metabolite enriched gene cluster). Taken together, the genomic resource obtained from the present study form a valuable repository of genetic information for elucidating and exploring the secondary metabolic circuitry of Plantagos. Further, information on the regulatory aspects of genes related to secondary metabolites, shall aid in the enhanced production of valuable metabolites in these plants.

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