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        Oxygen and Nitrogen Reactive Species Are Effectively Scavenged by Eucalyptus globulus Leaf Water Extract

        Isabel F. Almeida,Eduarda Fernandes,José L.F.C. Lima,Patrícia Valentão,Paula B. Andrade,Rosa M. Seabra,P.C. Costa,M.F. Bahia 한국식품영양과학회 2009 Journal of medicinal food Vol.12 No.1

        Eucalyptus globulus Labill. (Family Myrtaceae) is a plant of Australian origin, with a reported therapeutic use in airway inflammatory diseases. Considering that reactive oxygen species (ROS) and reactive nitrogen species (RNS) have been implicated in the pathogenesis of airway inflammatory diseases such as asthma and chronic obstructive pulmonary disease, an effective scavenging activity against these reactive species may contribute for the therapeutic effect of this plant. In the present study, a water extract of E. globulus leaves was evaluated for its putative in vitro scavenging effects on ROS (HO·, O2·−, ROO·, and H2O2) and RNS (·NO and ONOO−) and on 2,2-diphenyl-1-picrylhydrazyl radical (DPPH). Qualitative and quantitative analyses of the extract's phenolic composition were also performed. The Eucalyptus leaf water extract presented a remarkable capacity to scavenge all the reactive species tested, with all the 50% inhibitory concentrations being found at the μg/mL level. Phytochemical analysis showed the presence of polyphenols such as flavonoids (rutin and quercitrin) and phenolic acids (chlorogenic acid and ellagic acid), which may be partially responsible for the observed antioxidant activity. These observations provide further support, beyond the well-known antibacterial and antiviral activities of the Eucalyptus plant, for its reported use in traditional medicine such as in the treatment of airway inflammatory diseases, considering the important role of ROS and RNS in the inflammatory process, although further studies are needed to prove the bioavailability of the antioxidants/antibacterial compounds of the extract as well as the ability of the active compounds to reach specific tissues and to act in them.

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        Enhancement of Microbial Oil Production by Alpha-linolenic Acid Producing Yarrowia lipolytica Strains QU22 and QU137

        Paula Mattanna,Priscila Dallé da Rosa,Ana Paula Gusso,Neila S. P. S. Richards,Patrícia Valente 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.6

        Microbial oils enriched in essential polyunsaturatedfatty acids can be used as nutritionalcomplements. In order to improve the lipid yields and lipidprofiles of 2 Yarrowia lipolytica strains, the effects ofmedium components and culture conditions were investigatedusing shake flasks. Under optimized conditions (cultivationfor 3 days at 25ºC and 150 rpm, using peptone as anitrogen source and glucose as a carbon source), thebiomass, lipid yield, and lipid content reached 6.75 g/L,3.48 g/L, and 51.55% for Y. lipolytica QU22, and 6.85 g/L,3.52 g/L, and 51.38% for Y. lipolytica QU137, respectively. Using a shaking speed of 150 rpm, unsaturated fatty acidsconstituted 87.96% (QU22) and 87.08% (QU137) of thetotal lipids produced by these strains, with 9.52 and 7.86%of alpha-linolenic acid, respectively. The strains are suitablecandidates for fermentation processes involving essentialpolyunsaturated fatty acid (PUFA) production.

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