잣나무의 Callus 誘起와 그 染色體 및 同位酵素變異에 關한 硏究

金佑龍 진주산업대학교 1986 論文集 Vol.24 No.-

잣나무(Pinus koraicusis)의 成熟胚를 G.D.와 W.S.의 基本培地에 여러 가지 生長調節物質을 添加하여 callus를 誘起시켜, callus의 誘起 및 生長에 適正한 培地를 究明함과 同時에 培養 callus의 染色體變異와 同位酵素變異를 調査한 結果를 要約하면 다음과 같다. 1. 照明時間은 9時間과 15時間을 處理하였는데 모두 callus 生長에서 별로 影響을 주지 않았다. 2. G. D. 基本培地에 生長調節物質을 高農度(2.0ppm)로 添加한 것은 callus의 生長沮害現象이 일어났고 低濃度(0.2ppm)을 添加한 것은 높은 生長率을 나타내었다. 3. G. D. 基本培地에 있어서 auxin과 cytokinin의 相互作用은 生長調節物質을 單獨處理한 것 보다 複合處理한 것이 좋은 成績을 나타내었다. 4. 잣나무 成熟胚의 callus 培養은 W. S. 基本培地보다 G. D. 基本培地가 더욱 適合하였다. 5. 各 培地別 染色體 調査에서는 M-3培地(, 4-D 0.1ppm+BAP 0.1 ppm)에서 가장 많은 正當細胞分裂이 일어났고 正數體의 比率은 M-3(61.9%), M-2(58.6%), M-4(48.5%), M-1(45.8%)의 順으로 나타났다. 6. 2倍體細胞는 auxin 單獨 또는 複合培地인 M-1과 M-2 培地보다 auxin과 cytokinin을 複合處理한 M-3와 M-4 培地에서 많이 나타났다. 7. 異常細胞分裂의 一種인 Tertrapolar spindles가 M-2 培地에서 發見되었다. 8. 同位酵素變異는 Peroxidase와 Esterase 두 酵素 다 같이 同一培地內 callus 간에는 變異가 없었으나 서로 다른 培地間 callus에서는 變異를 나타내었다. 9. Peroxidase 同位酵素의 밴드數는 M-3가 M-4 培地에서 보다 M-1과 M-2 培地에서 더욱 많이 出現되었고 Esterase 境遇는 M-1 培地에서 M-2, M-3,M-4 培地에서 더욱 많이 出現되었다. 10. 培地別로 banding pattern의 差異를 나타낸 것은 여러 가지 生長調節物質들이 callus 組織에 生理遺傳學的으로 作用하였기 때문이라 推定할 수가 있었다. Present studies were attempted to determine an optimum medium for the induction of callus using the embryo of Pinus koraiensis as material and using G. D. and W. S. as basal media. The variations in chromosome numbers of the cultured calli and differences in isozyme patterns of the calli cultured on different media were also examined. The results of these experiments were summarized as follows: 1. Difference in the duration of illumination, 9 and 15 hours, had no particular effect upon the growth of callus. 2. High concentration(2.0ppm) of the growth regulators usually inhibited the callus growth, while low concentration(0.2ppm) showed rapid growth rate. 3. Combination of auxin and cytokinin gave better result than the single use of them. 4. The callus growth rate was found to be better on G. D. basal medium than on W. S. medium. 5. The normal cell division was found most frequent on M-3 medium, while the occurrence of euploids in callus was 61.9% on M-3, 56.6% on M-2, 48.5%on M-4 and 45.8% on M-1 medium. 6. The number of normal diploid cell was observed more in M-3 and M-4 than in M-1 and M-2. 7. Tetrapolar spindles resulting from the abnormal divisions were found occurring in M-2 medium. 8. The banding patterns of peroxidase and esterase were similar to on another among different calli cultured with the same media, but were varied in different media. 9. The number of bands of peroxidase was fewer in M-3 and M-4 than in M-1 and M-2, and that of esterase was fewer in M-2, M-3 and M-4 than in M-1. 10. In could the assumed that the difference in banding patterns at each medium was caused by various growth regulators which inflicted genetical as well as physiological effects upon callus tissues.

Robinia pseudoacacia外 三種의 組織培養에 關한 硏究

金佑龍 진주산업대학교 1976 論文集 Vol.14 No.-

In order to substract the time and cost of propagation for inducing the haploid plants per each species, 500 anthers of 1ate uninucleate microspore or early binucleate microspore stage of Robinia pseudoacacia(Fuel tree), Punica granatum(Ornamenta1 tree), Aleurites fordii(Faty tree) and Styrax japonica(Silvicutural tree) were cultured on the modified Murashige and Skoog's medium supplemented with Kinetin, 2. 4-D and NAA as growth regulators. And I observed the samples of cultured anthers under the microscope which were made by Microtoming method and Paraffine method. The results were summarized as follows. 1) Among 500 cultured anthers per each species anther numbers inducing the diploid callus were as follows; Styrax japonica 20(4%for the species total). Aleurites fordii 10 (2%for the species total) and Punica granatum 45(9% for the species tota1) were showed. 2) 2n Callus were induced from anther wall, but haploid callus were induced from anther loclue 3) Haploid cal1us were induced only in 25 anthers(5% forthe species total) of Robinia pseudoacacia. 4) These haploid callus were not originated from body cell of anther wa1l tissue, but from reduced microspores. 5) Since already reported many herbaceous haploid plants were induced from the callus which were originated from reduced microspores, I conclude that the anther of woody plant which induced the haploid callus aslo will be cultured aploid plant.

진주산업대학교의 樹木相과 管理에 관한 소고

김우룡,안현철,조현서,추갑철,송재철 진주산업대학교 농업기술연구소 2001 農業技術硏究所報 Vol.14 No.-

This study was carried out to investigate the woody plants of the Chinju National University. Field survey was carried out 3 times from July to October 2000. The results obtained from this study were as follows : 1. Among 145 kinds, 88 genera, 53 families of total woody plants, the needle-leaved trees of 28 kinds, 15 genera, 6 families and broad-leaved trees of 117 kinds, 73 genera 47 families were found. 2. Among the 4 investigated districts, area II showed the most abundant woody plants(92 kinds). 3. The endemic woody plants to Korea in Chinju National University were 6 species, They are Pinus koraiensis, Abies koreana, Buxus microphylla var. koreana, Cornus walteri, Carpinus coreana, Gleditsia joponica var. koraiensis. 4. The species of rare and endangered plants were 6 species, There are Corylopsis coreana, Juniperus chinensis var. sargentii, Koelreuteria paniculata, Abies koreana, Magnolia kobus, Tsuga siebodii and The exotic woody plants in korea were 59 kinds 41 genera 28 families. 5. The most abundant populations were Thea sinensis(271 populations) and Ilex crenata(247 populations).

闊葉樹의 몇가지 種子形質에 對한 soft X-ray의 利用

金佑龍 진주산업대학교 1981 論文集 Vol.19 No.-

本 硏究는 被子植物 31種에 대한 soft X-ray photography에 依한 種子의 몇가지 形質을 調査하기 爲하여 識驗한 結果, 種子의 大小, 種皮의 厚薄에 關係없이 胚와 胚芽의 發達程度, 子葉의 크기와 着生部位, 그리고 子種子의 有無와 그의 着生狀態等의 種子의 充實性과 病蟲害, 그리고 機械的 傷害等에 對한 選擇的 感受性이 있어 識判別에 有效하다고 할 수 있다. To find out, if soft X-ray photography could be utilized for studying some characters of seeds for 31 species of angiosperms, these investigations for the fullness, damages by blight and harmful insects and the degree of mechanical damages of those seeds by examining the degree of development of embryo and endosperm, size and position of cotyledon, existence or nonexistence and position of twin seed were performed. The results showed that the selective sensitivity of them could be recognized without relation to the size of seeds and thickness of seed coat. Therefore I conclude that it is possible to use soft X-ray photography reliably to find out the various characters of seeds.

産地別 해송種子의 特性에 關한 分析

金佑龍 진주산업대학교 1975 論文集 Vol.13 No.-

産地에 따른 해송種子의 特性을 究明하기 위하여 本實驗을 遂行하였던바 그 結果를 요약하면 다음과 같다 1. 産地 모두 同一하게 毬果當 種子數와 種子充實率間에는 相關關係가 없었으며 毬果當 種子數와 毬果當 種子重量間에는 高度의 相關關係를 보였고 種子充實率과 毬果當 種子重量間에서도 相關이 없었다. 2. 産地別 해송種子長과 種子幅은 사천이 가장 놓은 값을 보였으며 다음은 거제, 충무의 順으로 되고 가장 낮은 값은 남해였으며 變異의 幅은 비교적 적었다. 3. 産地에 의한 種子長, 種子幅을 分散分析한 結果産地間은 모두 高度의 有意性이 認定되고 反覆間에는 有意差가 없었다. 4. 産地別 平均値間의 有意差를 多重檢定하였던바 種子長에 있어서는 거제와 충무間은 有意性이 없었고 다른 産地間은 有意性이 있었으며 種子幅에 있어서는 사천은 거제, 거제는 충무, 충무는 남해間에는 有意性이 없었다. 5. 産地에 따라 種子長과 種子幅間의 相關關係는 모두 同一한 傾向을 보이지는 않았으나 平均高度의 相關을 보였다. 6. 全産地를 通하여 볼 때 種子長의 出現率은 6.0mm이 가장 높게 보였으며 種子幅은 대체로 3.5mm가 가장높게 出現率을 나타내고 있다. These experiments were carried out to know analysis on the characters of Pinus thunbergii seeds by locations. The results were summarized as follows: 1. The coefficient correlations between the number of seed per cones and the weight of seed per cones showed to be high signigicant at every locations, and the percentage of fullness seeds did not showed. The coefficients correlations between the percentage of fullness seeds and the weight of seed per cones did showed. 2. The seed length and the seed width showed to be the highest in Sa-Chon, and the lowest in Nam-hae. The coefficients variation were low comparatively. 3. The results of analysis of variance on seed length and seed width by locations showed highly significant in all locations. 4. The results of Duncan's multiple range test on seed length by locations were different of significance in other location, except in Kue-je and Chung-mu. The seed width by locations showed not different of significance in Sa-chon and Kue-je, Kue-je and Chung-mu, Chung-mu and Nam-hae. 5. The correlation coefficients between seed length and seed width showed not identical tendency in all locations, but the average of Correlation coefficients were highly significant. 6. The frequency of seed length showed to be the most 6.0mm of all locations, and the frequency of seed width showed to be the most 3.5mm of all locations.

樹冠通過雨, 樹幹流, 林外雨의 AI, Mn, Ni 濃度의 變化

金佑龍,李總揆,金鍾甲 진주산업대학교 농업기술연구소 1999 農業技術硏究所報 Vol.12 No.-

This study was carried out to investigate on heavy metals by throughfall, stemflow, rainfall at survey area. The concentration in Al by forest type was higher 1.3 times of Pinus thunbergii than Quercus spp. at industrial area, and was high 2.1 times at urban area. Stemflow was higher 2.3 times, 113 times respectively than throughfall, rainfall at industrial and urban area. Rainfall was lowed at industrial and urban area. The concentration in Mn by forest type was higher 2.3 times of Pinus thunbergii than Quercus spp. urban area. Concentration by rain were the highest in stemflow, and order of throughfall, rainfall. Concentration seasonal changes were the highest on December at industrial area, and lowed order on March, June, August, Seasonal changes were not remarkable at urban area. Heavy metals concentration by survey area ordered of Al>Mn>Ni at the industrial area, and those of ordered of Mn>Al>.Ni

智異山 들쥐의 生態 및 食餌物 分析에 關한 硏究

金佑龍,李總揆 진주산업대학교 산업과학기술연구소 2006 산업과학기술연구소보 Vol.- No.13

지리산 들쥐류의 종류와 식이물을 분석하여 야생동물의 생태에 관한 기초자료를 제공하기 위하여 실시하였다. 포획된 들쥐류의 종류는 대륙밭쥐와 흰넓적다리붉은쥐, 등줄쥐, 땃쥐, 뒤쥐 등 5종류였다. 포획된 들쥐의 총 개체수는 529개체였고, 대륙밭쥐가 318개체로서 제일 많았다. 흰넓적다리붉은쥐(119), 등줄쥐(83), 땃쥐(5), 뒤쥐(4)순이었다. 흰넓적다리붉은쥐는 산림의 근경과 종실, 곤충 등을 섭식하는 식성이었다. 대륙밭쥐는 녹색 섬유질을 섭식하는 잡식성 이었다. 체중의 범위는 등줄쥐가 15g~50g, 흰넓적다리붉은쥐가 10g~50g, 대륙밭쥐가 15g~70g이었다. In order to survey the species of field mice and analyze on feces of them in Mt. Jiri, Fire different the capturable boxes of field mice was instaued from 500m to 1900m by altitude. 5 species of field mice and total of 529 individuals were collected at surveyed areas. The individuals of Clethrionomys rufocanus regulus were the most captured as 318 individuals, and in order of 119 on Apodemus speciosus penisulae, 83 on Apodemus agrarious Coreae, 5 on Crocidura suavelens shantrungensis, and 4 on Sorex caecutiens annexus. The feeding habits of A. speciosus penisulae showed to take root stocks, seeds and insects. The feeding habit of C. rufocanus regulus having a taste for vegetable fibers was omnivorous . The range of body weight were from 15 g to 50 g in A. agrarious, 10 g-50 g in A. speciosus, and 15 g-70 g in C. fufocanus, respectively.

섬잣나무의 胚培養에 미치는 培地조성과 植物生長 調範劑의 영향

김우룡,김돌이,강호종,김영두 진주산업대학교 농업기술연구소 1992 農業技術硏究所報 Vol.5 No.-

섬잣나무의 胚로부터 器內胚培養을 통하여 온전한 植物體를 유기시키기 위해 培地의 造成과 植物 生長調節劑의 影響을 儉素하였다. 1. 基本 培地로서 木本類의 組織培養에 양호한 GD, MS. LM배지를 기본으로 하여 實驗한 結果 GD와 MS가 섬잣나무의 胚培養에 적당하였다. 2. GD, MS, LM 배지에 auxin, cytokinin으로서 0.1ppm, 0.5ppm, 0.05ppm을 添加하여 4周間 培養하였을때 주로 GD배지에서 NAA 0.1ppm, MS배지에서는 2, 4-D 0.1ppm에서 Shoot 生長이 매우 좋은 結果를 나타냈다. 3. 식물체 유기를 위해서는 Callus段階를 거치지 않고 生長하는 胚가 있는 반면 植物 生長調節劑에 따라 Callus화 되는 胚가 있는데 현재 Callus에서 體細胞 胚 유도를 위해 4개월 동안 배양하고 있는데, 이의 연구는 섬잣나무의 組織培養을 利用 大量增殖에 많은 도움을 줄 것이다. In order to induce a complete plant from embryo of Pinus Parviflora GD, MS, LM media and plant regulators such as 2, 4-D, NAA, 2, 4-D+BAP were employed for in vitro culture media. 1. The best condition for the embryo culture was GD and MS system. 2. When the addition of plant growht regulators (0.5, 0.1, 0.05ppm) in the GD, MS and LM media, generaly GD medium supplemented with 0.1ppm of NAA, MS medium supplemented with 0.1ppm of 2, 4-D showed the best result. 3. Present studies have been investigated of callus for four months, and going to keep on inducing somatic embryo in vitro embryo culture.

몇가지 針葉樹에 있어서 初生葉의 形態的 特性에 關한 硏究

金佑龍 진주산업대학교 1979 論文集 Vol.17 No.-

리기다소나무, 해송, 낙엽송에 있어서 初生葉의 形態的 特性을 究明하기 爲하여 葉長, 氣孔線數, 鋸齒數를 調査하였던 바 그 結果를 要約하면 다음과 같다. 1. 初生葉長은 리기다소나무가 平均 3.25, 해송은 1.99, 낙엽송은 2.14㎝로 나타내었으며 이들 間에는 有意性이 認定되었다. 2. 氣孔線數에 있어서는 大體로 3∼8線의 Range를 보였으며 樹種間에 有意性은 認定할 수 없었다. 3. 鋸齒數에 있어서는 樹種間에 有意性을 認定할 수 있었으며, 樹種 모두 變異係數는 높은 값을 나타내었다. 4. 樹種別 初生葉形質의 相關關係는 樹種間에 다른 傾向의 結果를 가져 왔다. There studies were carried out to know the characteristics of morphological length of needles, numbers of the stomata lines, numbers of serrations of juvenile needles by 1-0 Pinus rigida seedlings, 1-0 Pinus thunbergii seedlings and 1-0 Larix leptolepis seedlings. The results are summarized as follows. 1. The average numbers of juvenile needles length were 3.25㎝ in the case of 1-0 Pinus rigida seedlings, were 1.99㎝ in the case of 1-0 Pinus thunber gii seedlings and were 2.14㎝ inthe case of 1-0 Larix leptolepis seedlings, and there were significantly different in these trees. 2. In this observation, the range of stomata line number was 3 to 8, and it showed not different of significance in trees. 3. In the serration number, there was a significant difference in trees, and the coefficient variation was high in these trees. 4. The coefficient correlations of some characters of juvenile needles in 1-0 Pinus rigida seedlings, 1-0 Pinus thunber gii seedlings and 1-0 Larix leptolepis seedlings did not show identical tendency in these trees.

벗나무 種子의 發芽와 성분에 미치는 온도의 影響

金佑龍 진주산업대학교 1977 論文集 Vol.15 No.-

The seed of Prunus campanulata, growing wild in Japan shows a low germination percentage and the natural seedlings do not occur often. It seems to the authors that this may be attributed to germination temperature, storage temperature, or chemical components. The causality in the cherry seeds, including Prunus yedoensis seed from Chinju district, was investigated. Optimum temperature for the germination of Prunus campanulata seed was obtained at 7℃ in the preliminary experiment. After the storage of both seeds at 7℃, 20℃, and 30℃ during the period of four months, the germination(at 7℃) and the chemical components of seeds (oil, sugar, and protein) were tested. The oil content showed a positive, high correlation to the germination percentage, but the contents of sugars and protein did not. The germination percentage is presumably possible to be estimated from the change in the oil content in the seed.