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RISS 인기검색어
- 검색키워드
- 저자 : Nikolas Fokialakis (검색결과 3 건)
- 무료
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Antioxidant and Cytotoxic Activity of the Wild Edible Mushroom Gomphus clavatus
Maria Makropoulou,Nektarios Aligiannis,Zacharoula Gonou-Zagou,Harris Pratsinis,Alexios-Leandros Skaltsounis,Nikolas Fokialakis 한국식품영양과학회 2012 Journal of medicinal food Vol.15 No.2
The fruiting bodies of the edible mushroom Gomphus clavatus (Family Gomphaceae) were collected from the wild and extracted with solvents of increasing polarity. Crude extracts were evaluated for their total phenolic content, their antioxidant capacity, and their cytotoxic activity against MCF-7 and PC-3 cancer cell lines. Concerning total phenolics and antioxidant activity, the methanol extract showed the most potent radical scavenging activity with inhibition of 45.5% of 1,1-diphenyl-2-picrylhydrazyl at 3mg/mL. Further chemical investigation of the methanol extract led to the isolation and identification of nine compounds, among them four ergosterol derivatives. Concerning cytotoxicity, the dichloromethane (DCM)extract showed the most interesting activity, with half-maximal inhibitory concentration (IC50) values of 55.3 and 49.0 lg/mL in the MCF-7 and PC-3 cell lines, respectively. Further investigation of the DCM extract lead to the identification of methyl esters of fatty acids and the isolation of four fatty acids and three ergosterol derivatives. Ergosterol peroxide (compound 6) was one of the most active constituents, with IC50 values of 35.8 lM and 30.6 lM for MCF-7 and PC-3 cells, respectively, suggesting that the cytotoxic activity of the crude extract could be at least partly attributed to the presence of ergostan derivatives. Those findings suggest that G. clavatus can be considered as a medicinal food with antioxidant and chemopreventive activities.
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Antioxidant Properties of the Wild Edible Mushroom Lactarius salmonicolor
Georgios Athanasakis,Nektarios Aligiannis,Zacharoula Gonou-Zagou,Alexios-Leandros Skaltsounis,Nikolas Fokialakis 한국식품영양과학회 2013 Journal of medicinal food Vol.16 No.8
The fruiting bodies of the edible mushroom Lactarius salmonicolor (Russulaceae) collected from the wild were extracted with solvents of increasing polarity and evaluated for their antioxidant capacity by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method. Additionally, their total phenolic content was evaluated by a modification of the Folin-Ciocalteu method. The methanol extract showed the most potent radical scavenging activity (inhibition of 36.7% of DPPH at 3 mg/mL and 6.8 mg gallic acid equivalents/g of dry extract), and was further fractionated. Total phenolics and antioxidant activity found to be significant higher in the fractions of increased polarity. The phytochemical investigation which was performed by chromatographic and spectrometric techniques led to the isolation of nine compounds from the methanol extract and the identification of 10 fatty acids and fatty acids esters from the dichloromethane extract. Evaluation of the antioxidant activity of isolated compounds showed p-hydroxybenzoic acid (4) to be the most active. Among the various compounds found, the rare azulene type 7-acetyl-4-methylazulene-1-carboxylic acid (1) has been identified, which had been isolated only once before from L. deliciosus. Therefore, this compound seems to have some chemotaxonomic value for Lactrarius species exuding orange to red milk.
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Nikolaos Tsafantakis,Efrosini S. Katsanou,Katerina Kyriakopoulou,Eirini-Christina Psarou,Iliana Raptaki,Alexios L. Skaltsounis,Marc Audebert,Kyriaki A. Machera,Nikolas Fokialakis 한국식품영양과학회 2019 Journal of medicinal food Vol.22 No.12
Opuntia ficus indica has been an important dietary source and a traditionally used medicinal plant. Given the promising health-promoting properties of this plant, a comparative toxicological assessment and antioxidant bioevaluation of extracts from different parts of the plant were carried out in relation to their chemical profile. Toxicity was examined at multiple endpoints using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), Comet and the γH2AX In-Cell Western Assay, while hyphenated ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS) analysis was carried out to identify main constituents. None of the extracts showed any cytotoxic and genotoxic effect on cell lines used, apart from the flower extract in HepG2 cells at the highest concentration tested (2.5 mg/mL). Both fruit flesh and seed extracts demonstrated a prominent protective effect against H2O2-induced genotoxicity in almost all concentrations tested, while extracts originated from flowers and cladodes were effective only at the low non-cytotoxic (0.312 and 0.625 mg/mL) and high (1.25 and 2.5 mg/mL) concentrations, respectively. In total, 2 phenolic acids, 12 flavonoids, along with 3 feruloyl derivatives and the plant pigment indicaxanthin, were tentatively identified by UHPLC-HRMS analysis. Phenolic acids (compounds 1 and 2) were mainly distributed in cladodes (64.6%), while flavonoids (3–14) in the flowers (81.8%). Overall, the highest amount of total flavonoids (22.76 ± 0.015 mg of quercetin equivalent [QE]/g) and total phenolics (62.80 ± 0.009 mg gallic acid equivalents [GAE]/g) was found in the flower extract. Flavonoid glycosides have not been detected in the seeds and the flesh, while the fruit seed extract contained mainly feruloyl derivatives. Our data provide convincing evidences for the lack of cytotoxic and genotoxic effects of O. ficus indica aqueous extracts and, in parallel, support the potential for further exploitation of this plant in the food supplement or functional food sector.
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