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        In vitro rapid propagation technology system of Dendrobium moniliforme (L.) Sw., a threatened orchid species in China

        Liu Xu,Sun Liyong,Nie Tangjie,Chen Yao,Yin Zengfang 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.3

        Dendrobium moniliforme is a threatened and medicinal orchid species in China. The establishment of in vitro rapid propagation technology system will facilitate preservation and utilization of germplasm resources in D. moniliforme. In this study, the effects of different plant growth regulators (PGRs) on seed germination, proliferation of cluster shoots, rooting of shoots were tested under asymbiotic culture condition, and the best acclimatization condition of plantlets was screened. Our research results were shown as follows: germination rate (GR) was significantly increased by adding N6-benzyladenine (BA, 0.1 mg/L) during asymbiotic culture. In Murashige and Skoog (MS) medium supplemented with α-naphthaleneacetic acid (NAA, 1.0 mg/L) and BA (1.0 mg/L), the proliferation coefficient of cluster shoots was 6.1 times comparing with the control group. The MS medium with NAA (0.5 mg/L) and indole-3-butyric acid (IBA, 0.5 mg/L) promoted the highest rooting rate (RR). For plantlet transplanting, the equal mixing volume ratio of pine bark, turfy soil, and peanut shells was the best substrate. In a word, this rapid propagation system provides strong technical support for D. moniliforme to expand proliferation and germplasm resource protection.

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