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Vandana Bisht,Vineeta Chattree,,Neena Khanna,,D.N. Rao 한국물리학회 2005 Current Applied Physics Vol.5 No.2
Leprosy, a debilitating disease shows manifestations ranging from strong cell mediated immune (CMI) response in tuberculoidform (BT/TT) or weak CMI with immunological anergy in lepromatous form (BL/LL). Repeated stimulation with mycobacterialantigen causes activation induced cell death (apoptosis) or anergy in these patients. Previous studies showed in vitro T cell prolif-eration and increased Th1 response with liposomal (particulate) presentation of mycobacterial antigens with immunomodulators(MDP analog, murabutide and T cell peptide of Trat protein). In the present study, the role of caspases; expression of apoptoticmarkers, CD95 and CD95L in T cells of leprosy patients and the eect of particulate formulations in the reversal of apoptosisin BT/TT and BL/LL patient group was studied. Caspase 8/3 activity was maximum (p<0.01) in BL/LL patients in constitutivestate, which was followed by decreased activity in BT/TT and normal subjects. Caspase 8/3 activity increased signicantly(p<0.05) from constitutive state after 5 days of antigen stimulation (inducible state). Both the antigens were equally potent in induc-ing the apoptosis in leprosy patients. A decreased caspase activity using the particulate formulations of both antigens was observedin BL/LL group. The expression of CD95 and CD95L in T cells of leprosy patients increased signicantly after 5 days of antigenstimulation. However, % expression of apoptotic markers by T cells of BL/LL patients decreased signicantly (p<0.05) when theparticulate formulations were used. To summarize, the particulate formulations of antigens with immunomodulators slowed downthe apoptosis and hence T cell anergy in leprosy by stimulating the cells to proliferate..