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- 저자 : Nam‑Hai Chua (검색결과 8 건)
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김상희,이정근,양준이,정춘균,Nam-Hai Chua 한국식물학회 2013 Journal of Plant Biology Vol.56 No.1
Histone modifications are known to play importantroles in plant development through epigenetic regulation ofgene expression. How these modifications regulate downstreamtargets in response to various environmental cues anddevelopmental stimuli is still largely unknown. Here, weprovide evidence that Arabidopsis histone H3K4 methyltransferaseSET DOMAIN GROUP2 (SDG2) is required for fullactivation of hormone responsive genes upon hormonetreatment. The pleiotropic phenotypes of sdg2 were closelyrelated to those of auxin deficient mutants and RNA analysisrevealed that expression of early hormone responsive geneswas significantly reduced in sdg2-5. By ChIP analyses wefound that H3K4 tri-methylations on chromatin region ofhormone responsive genes such as SAUR27, KIN1 andGASA6 were enriched in WT upon hormone treatmentswhereas these enrichments were largely abolished in sdg2-5. After hormone treatment, chromatin regions of responsivegenes that accumulated H3K4me3 in WT overlapped withthose displaying decreased H3K4me3 levels in sdg2-5. Histone H3K4 di-methylation levels on tested genes wereincreased rather than decreased in sdg2-5, suggesting thatSDG2 mediates transition of H3K4me2 to H3K4me3. Takentogether, we conclude that the SDG2 activity is required toregulate the expression of hormone responsive genes viahistone H3K4 tri-methylation.
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PLANT U‑BOX PROTEIN 10 negatively regulates abscisic acid response in Arabidopsis
서준성,Pingzhi Zhao,정춘균,Nam‑Hai Chua 한국응용생명화학회 2019 Applied Biological Chemistry (Appl Biol Chem) Vol.62 No.-
MYC2 is well known as a positive regulator for abscisic acid (ABA) signaling but whether PLANT U-BOX PROTEIN 10 (PUB10) is involved in ABA responses has not been reported. Here, we show that the E3 ubiquitin ligase PUB10 modulates ABA signaling in Arabidopsis. PUB10ox (35S:PUB10-myc) and myc2 loss-of-function mutants were hyposensitive to ABA during germination, whereas pub10 loss-of-function and MYC2ox (35S:MYC2-GFP) mutants were hypersensitive. In addition, pub10 mutants showed hypersensitivity to high salt and osmotic stress during germination; by contrast, PUB10ox line displayed the opposite phenotype. ABA-induced expression of KIN2 (Cold- and ABA-Inducible Protein), RD22 (Responsive to Dehydration 22), ANAC019 (NAC Domain-Containing Protein 19), and ANAC055 (NAC Domain- Containing Protein 55) was enhanced in both pub10 and MYC2ox plants. Taken together, pub10 plants phenocopied MYC2ox plants, whereas PUB10ox plants phenocopied myc2 in ABA response. Our results provide evidence that PUB10 negatively regulates ABA signaling in Arabidopsis.
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The Deubiquitinating Enzymes UBP12 and UBP13 Positively Regulate MYC2 Levels in Jasmonate Responses
Jeong, Jin Seo,Jung, Choonkyun,Seo, Jun Sung,Kim, Ju-Kon,Chua, Nam-Hai American Society of Plant Biologists 2017 The Plant cell Vol.29 No.6
<P>The transcription factor MYC2 has emerged as a master regulator of jasmonate (JA)-mediated responses as well as crosstalk among different signaling pathways. The instability of MYC2 is in part due to the action of PUB10 E3 ligase, which can polyubiquitinate this protein. Here, we show that polyubiquitinated MYC2 can be deubiquitinated by UBP12 and UBP13 in vitro, suggesting that the two deubiquitinating enzymes can counteract the effect of PUB10 in vivo. Consistent with this view, UBP12 and UBP13 associate with MYC2 in the nucleus. Transgenic Arabidopsis thaliana plants deficient in UBP12 and UBP13 show accelerated decay of MYC2 and are hyposensitive to JA, whereas plants overexpressing UBP12 or UBP13 have prolonged MYC2 half-life and are hypersensitive to JA. Our results suggest that there is a genetic link between UBP12, UBP13, and MYC2. Our results identify UBP12 and UBP13 as additional positive regulators of JA responses and suggest that these enzymes likely act by stabilizing MYC2.</P>
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Wang, Huan,Chung, Pil Joong,Liu, Jun,Jang, In-Cheol,Kean, Michelle J.,Xu, Jun,Chua, Nam-Hai Cold Spring Harbor Laboratory Press 2014 Genome Research Vol.24 No.3
<P>Recent research on long noncoding RNAs (lncRNAs) has expanded our understanding of gene transcription regulation and the generation of cellular complexity. Depending on their genomic origins, lncRNAs can be transcribed from intergenic or intragenic regions or from introns of protein-coding genes. We have recently reported more than 6000 intergenic lncRNAs in <I>Arabidopsis</I>. Here, we systematically identified long noncoding natural antisense transcripts (lncNATs), defined as lncRNAs transcribed from the opposite DNA strand of coding or noncoding genes. We found a total of 37,238 sense–antisense transcript pairs and 70% of annotated mRNAs to be associated with antisense transcripts in <I>Arabidopsis</I>. These lncNATs could be reproducibly detected by different technical platforms, including strand-specific tiling arrays, Agilent custom expression arrays, strand-specific RNA-seq, and qRT-PCR experiments. Moreover, we investigated the expression profiles of sense–antisense pairs in response to light and observed spatial and developmental-specific light effects on 626 concordant and 766 discordant NAT pairs. Genes for a large number of the light-responsive NAT pairs are associated with histone modification peaks, and histone acetylation is dynamically correlated with light-responsive expression changes of NATs.</P>
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Chung, Pil Joong,Park, Bong Soo,Wang, Huan,Liu, Jun,Jang, In-Cheol,Chua, Nam-Hai American Society of Plant Biologists 2016 Plant Physiology Vol.170 No.3
<P>Expression of many plant microRNAs is responsive to hormones and environmental stimuli, but none has yet been associated with light. Arabidopsis (Arabidopsis thaliana) miR163 is 24 nucleotides in length and targets mRNAs encoding several S-adenosyl-Metdependent carboxyl methyltransferase family members. Here, we found that miR163 is highly induced by light during seedling deetiolation as well as seed germination. Under the same condition, its target PXMT1, encoding a methyltransferase that methylates 1,7-paraxanthine, is down-regulated. Light repression of PXMT1 is abolished in a mir163 null mutant, but the repression can be restored to wild-type levels in complementation lines expressing pri-miR163 gene in the mir163 mutant background. During seed germination, miR163 and its target PXMT1 are predominantly expressed in the radicle, and the expression patterns of the two genes are inversely correlated. Moreover, compared with the wild type, mir163 mutant or PXMT1 overexpression line shows delayed seed germination under continuous light, and seedlings develop shorter primary roots with an increased number of lateral roots under long-day condition. Together, our results indicate that miR163 targets PXMT1 mRNA to promote seed germination and modulate root architecture during early development of Arabidopsis seedlings.</P>
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A membrane-bound NAC transcription factor regulates cell division in Arabidopsis.
Kim, Youn-Sung,Kim, Sang-Gyu,Park, Jung-Eun,Park, Hye-Young,Lim, Mi-Hye,Chua, Nam-Hai,Park, Chung-Mo American Society of Plant Physiologists 2006 The Plant cell Vol.18 No.11
<P>Controlled release of membrane-tethered, dormant precursors is an intriguing activation mechanism that regulates diverse cellular functions in eukaryotes. An exquisite example is the proteolytic activation of membrane-bound transcription factors. The proteolytic cleavage liberates active transcription factors from the membranes that can enter the nucleus and evokes rapid transcriptional responses to incoming stimuli. Here, we show that a membrane-bound NAC (for NAM, ATAF1/2, CUC2) transcription factor, designated NTM1 (for NAC with transmembrane motif1), is activated by proteolytic cleavage through regulated intramembrane proteolysis and mediates cytokinin signaling during cell division in Arabidopsis thaliana. Cell proliferation was greatly reduced in an Arabidopsis mutant with retarded growth and serrated leaves in which a transcriptionally active NTM1 form was constitutively expressed. Accordingly, a subset of cyclin-dependent kinase (CDK) inhibitor genes (the KIP-related proteins) was induced in this mutant with a significant reduction in histone H4 gene expression and in CDK activity. Consistent with a role for NTM1 in cell cycling, a Ds element insertional mutant was morphologically normal but displayed enhanced hypocotyl growth with accelerated cell division. Interestingly, cytokinins were found to regulate NTM1 activity by controlling its stability. These results indicate that the membrane-mediated activation of NTM1 defines a molecular mechanism by which cytokinin signaling is tightly regulated during cell cycling.</P>
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Kim, Joonki,Jung, Jae-Hoon,Reyes, Jose L.,Kim, Youn-Sung,Kim, Sun-Young,Chung, Kyung-Sook,Kim, Jin A.,Lee, Minsun,Lee, Yoontae,Narry Kim, V.,Chua, Nam-Hai,Park, Chung-Mo Wiley (Blackwell Publishing) 2005 The Plant journal Vol.42 No.1
<P>Class III homeodomain-leucine zipper proteins regulate critical aspects of plant development, including lateral organ polarity, apical and lateral meristem formation, and vascular development. ATHB15, a member of this transcription factor family, is exclusively expressed in vascular tissues. Recently, a microRNA (miRNA) binding sequence has been identified in ATHB15 mRNA, suggesting that a molecular mechanism governed by miRNA binding may direct vascular development through ATHB15. Here, we show that miR166-mediated ATHB15 mRNA cleavage is a principal mechanism for the regulation of vascular development. In a gain-of-function MIR166a mutant, the decreased transcript level of ATHB15 was accompanied by an altered vascular system with expanded xylem tissue and interfascicular region, indicative of accelerated vascular cell differentiation from cambial/procambial cells. A similar phenotype was observed in Arabidopsis plants with reduced ATHB15 expression but reversed in transgenic plants overexpressing an miR166-resistant ATHB15. ATHB15 mRNA cleavage occurred in standard wheat germ extracts and in Arabidopsis and was mediated by miR166 in Nicotiana benthamiana cells. miR166-assisted ATHB15 repression is likely to be a conserved mechanism that regulates vascular development in all vascular plants.</P>
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