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Nafiseh Tavakolpoor Saleh,Alireza Naderi Sohi,Elaheh Esmaeili,Somayeh Karami,Fatemeh Soleimanifar,Nikoo Nasoohi 한국생물공학회 2019 Biotechnology and Bioprocess Engineering Vol.24 No.6
Due to number of reasons such as ease of isolation and their broad differentiation capability, adult human mesenchymal stem cells (MSCs) are widely considered as of the most promising cells for regenerative medicine and tissue engineering. Nevertheless quick decrease in expression of transcription factors associated with stemness and self-renewal during ex vivo expansion of MSCs is an impediment against their therapeutic applications. Since the influence of extra cellular matrix (ECM) proteins on the fate of stem cells is well documented, the culture of MSCs on ECM-derived synthetic biomolecules is worth investigating. In the present study, a lamininderived peptide, YIGSR was covalently immobilized on the chitosan film surface using carbodiimide chemistry and confirmed by fluorometry. The results obtained from surface characterization by atomic force microscopy (AFM) and contact angle measurement, showed no significant difference in topological features and hydrophilicity after peptide immobilization. Employment of these surfaces for culture of human adipose-derived mesenchymal stem cells demonstrated that the immobilized YIGSR peptide has a favorable effect on adhesion and maintaining viability of the MSCs as well as on the expression of stemness markers (Nanog, Oct-4, and Sox-2) in these cells.
Nafiseh Tavakolpoor Saleh,Zohreh Hosseinzadeh,Narges Gholami Banadkuki,Maryam Salehi Novin,Sanaz Saljooghi Zaman,Tohid Moradi Gardeshi The Korean Society for Reproductive Medicine 2024 Clinical and Experimental Reproductive Medicine Vol.51 No.1
Objective: While sperm freezing (cryopreservation) is an effective method for preserving fertility, it can potentially harm the structure and function of sperm due to an increase in the production of reactive oxygen species. This study aimed to assess the impact of zinc oxide nanoparticles (ZnONPs) and selenium oxide nanoparticles (SeONPs) on various sperm functional parameters, including motility, plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), acrosome membrane integrity (ACi), and malondialdehyde (MDA) levels. Methods: Semen samples were collected from 20 Albino Wistar rats. These samples were then divided into six groups: fresh, cryopreservation control, and groups supplemented with SeONPs (1, 2, 5 ㎍/mL) and ZnONPs (0.1, 1, 10 ㎍/mL). Results: Statistical analysis revealed that all concentrations of SeONPs increased total motility and progressive reduction of MDA levels compared to the cryopreservation control group (p<0.05). However, supplementation with ZnONPs did not affect these parameters (p>0.05). Conversely, supplements of 1 and 2 ㎍/mL SeONPs and 1 ㎍/mL ZnONPs contributed to the improvement of PMI and ACi (p<0.05). Yet, no significant change was observed in MMP with any concentration of SeONPs and ZnONPs compared to the cryopreservation control group (p>0.05). Conclusion: The findings suggest that optimal concentrations of SeONPs may enhance sperm parameters during the freezing process.