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Shariatifar Nabi,Moazzen Maryam,Arabameri Majid,Moazzen Mojtaba,Khaniki Gholamreza Jehed,Sadighara Parisa 한국응용생명화학회 2021 Applied Biological Chemistry (Appl Biol Chem) Vol.64 No.S
The purpose of this study was to evaluate the concentration of PAHs and health risk in edible mushrooms using magnetic solid phase extraction (MSPE) and gas chromatography/mass spectrometry (GC/MS) method. Based on the results, the concentration of total PAHs was in the range from 0.82 to 6.25 μg/kg (with the means of 3.85 ± 0.24 μg/ kg). In this study, acenaphthylene (Ace) had the maximum mean level in mushroom samples (0.84 ± 0.19 μg/kg) and benzo(a)pyrene (BaP), acenaphthene (Ac), phenanthrene (Pa), dibenzo[a,h]anthracene (DahA), benzo(a)anthracene (BaA), fluoranthene (Fl), indeno[1,2,3-cd]pyrene (IP) and benzo[g,h,i]perylene (BgP) had minimum mean level (nondetected). The highest mean values of total PAHs (4.88 ± 0.30 μg/kg) was detected in grilled mushroom samples and lowest mean of total PAHs (2.77 ± 0.04 μg/kg) was detected in raw mushroom samples. The concentration of contamination in edible mushrooms was raw < fried > grilled, respectively. Furthermore, heat map visualization was applied to evaluate the relationship between the quantity and type of 16 PAHs in edible mushroom samples. Ultimately, the incremental lifetime cancer risk in edible mushrooms for adults and children was 6.85E-08 and 3.47E-07, respectively, which was lower than the acceptable risk limit ( 10–6) and all of the edible mushrooms sold in Tehran were considered safe for consumers.
Samaneh Goorani,Nabi Shariatifar,Niloofar Seydi,Akram Zangeneh,Rohallah Moradi,Behzad Tari,Farzaneh Nazari,Mohammad Mahdi Zangeneh 경희대학교 융합한의과학연구소 2019 Oriental Pharmacy and Experimental Medicine Vol.19 No.4
There are many medicinal plants in traditional medicine which are used to prevent, control, and treat anemia. One of these plants is Allium saralicum R.M. Fritsch. The purpose of our research was to investigate the efect of aqueous extract of A. saralicum leaf in the treatment of hemolytic anemia. In this study, 60 rats were used. Induction of hemolytic anemia was done by three injections of Phenylhydrazine in 50 animals. Then, the rats were divided into six subgroups, including negative healthy control, untreated negative control, and four groups receiving the A. saralicum at 25, 50, 100, and 200 mg/kg concentrations. At the end of day 15 of treatment, the animals of all groups were weight and then sacrifced. The blood, liver and spleen samples were drawn immediately to analyze the hematological, biochemical and histological parameters. All groups of A. saralicum (especially AS200) signifcantly (p≤0.05) reduced the raised concentrations of Fe, ferritin, erythropoietin, ALP, AST, ALT, GGT, cholesterol, LDL, triglyceride, total and conjugated bilirubin, urea, and creatinine and increased the levels of body weight, HDL, total protein, albumin, WBC, lymphocyte, monocytes, platelet, RBC, Hb, PCV, MCV, MCH, and MCHC as compared to the untreated group. Also, A. saralicum at all doses prevented pathological changes in the liver and spleen. In conclusion, because of aqueous extract of A. saralicum leaf anti-anemic property, it can be used as a medical supplement or drug.
Sharifiarab Gholamali,Mehraie Abbas,Arabameri Majid,Shariatifar Nabi,Mortazavian Amir M.,Moazzen Mojtaba,Sohrabvandi Sara 한국응용생명화학회 2022 Applied Biological Chemistry (Appl Biol Chem) Vol.65 No.3
Polycyclic aromatic hydrocarbons (PAHs) are dangerous environmental compounds that are sometimes found in food. The objective of present study was to measure the level of 16 PAHs in bottled water samples (non-carbonated or drinking, mineral, carbonated and carbonated flavored water) in Tehran by using magnetic solid-phase extraction and gas chromatography–mass spectrometry (MSPE/GC–MS) method. The limit of detections (LOD), limit of quantifications (LOQ) and recovery of PAH compounds were 0.010–0.210, 0.03–0.700 μg/L and 92.5–103.4%, respectively. The results showed that the mean of total PAHs in samples was 2.98 ± 1.63 µg/L and the mean of Benzo[a]pyrene (BaP) was 0.08 ± 0.03 µg/L, which were lower than standard level of the US-EPA (0.2 µg/L, BaP in drinking water). Also, our results showed that carbonated flavored water had maximum mean of total PAHs (4.95 ± 0.8 µg/L) and mineral water had minimum mean of total PAHs (1.24 ± 0.8 µg/L). The Monte Carlo method was applied to calculate the Estimated Daily Intake (EDI) and Incremental Life Cancer Risk (ILCR) indexes. In all samples, the rank order of the estimated CDI values based on the 95 percent percentile was F > B(a)A > Ace > Fl > Na > Ph > B(b)F > B(k)F > B(a)P > P > Ac > A. The cancer risk and uncertainty analysis of 95th Percentile for bottled waters studied gave values lower permissible limit of 10 −6 , indicating not pose a serious concern to humans.