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Analysis of Functional Ingredient Accumulation Mechanism and Application in Sake Yeast
Muneyoshi KANAI 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
S-adenosylmethionine (SAM) is a key regulatory compound in various biological reactions and functions as a major donor of methyl groups. SAM is highly accumulated in vacuoles in sake yeast compared to other yeast strains, bacteria, molds, or other microorganisms. To isolate a SAM-accumulating yeast strain, we screened methionine resistant strains using the yeast deletion library of budding yeast and identified ADO1 encoding adenosine kinase as one of the factors participating in high SAM accumulation. Next, to elucidate the mechanism underlying SAM accumulation in sake yeast strains compared with that in laboratory yeast strains, we performed quantitative trait locus (QTL: Quantitative trait locus) analysis and identified ERC1 gene, encoding a member of the multi-drug and toxin extrusion family having antiporter activity. Expression of the sake yeast ERC1 allele resulted in intracellular SAM accumulation. Moreover, the sake yeast ERC1 allele contributes to an increase in the ratio of Tetrahydrofolate (THF) to the total folate content in sake yeast. Thus, our findings might be useful in the breeding of high SAM and folate accumulating yeast strains for industrial uses such as dietary supplements, sake cakes and so on.