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        Heavy Metal Resistant Endophytic Fungi Isolated from Nypa fruticans in Kuching Wetland National Park

        Jenny Choo,Nuraini Binti Mohd Sabri,Daniel Tan,Aazani Mujahid,Moritz Müller 한국해양과학기술원 2015 Ocean science journal Vol.50 No.2

        Heavy metal pollution is an environmental issue globally and the aim of this study was to isolate endophytic fungi from mangrove wetlands of Sarawak to assess and test their ability to grow in the presence of various heavy metals (copper (Cu), zinc (Zn), lead (Pb), and chromium (Cr)). Samples of Nypa fruticans were collected from Kuching Wetland National Park (KWNP) for subsequent endophyte isolation. Ninety-three (93) isolates were obtained and assessed and the most resistant isolates (growing at concentrations up to 1000 ppm) were identified using fungal primers ITS 1 and ITS 4. All of the endophytic fungi were identified to be closely related to Pestalotiopsis sp. and this is to our knowledge the first study reporting the ability of Pestalotiopsis sp. to grow at high concentrations of copper, lead, zinc and chromium. Our results highlight the potential of using endophytic fungi for the treatment of heavy metal pollution, for example as biosorbents.

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        The Potential Roles of Bacterial Communities in Coral Defence: A Case Study at Talang-Talang Reef

        Felicity W. I. Kuek,Li-Fang Lim,Lin-Hui Ngu,Aazani Mujahid,Po-Teen Lim,Chui-Pin Leaw,Moritz Müller 한국해양과학기술원 2015 Ocean science journal Vol.50 No.2

        Complex microbial communities are known to exert significant influence over coral reef ecosystems. The Talang- Satang National Park is situated off the coast of Sematan and is one of the most diverse ecosystems found off-Sarawak. Interestingly, the Talang-talang reef thrives at above-average temperatures of 28- 30°C throughout the year. Through isolation and identification (16S rRNA) of native microbes from the coral, the surface mucus layer (SML), as well as the surrounding sediment and waters, we were able to determine the species composition and abundance of the culturable bacteria in the coral reef ecosystem. Isolates found attached to the coral are related mostly to Vibrio spp., presumably attached to the mucus from the water column and surrounding sediment. Pathogenic Vibrio spp. and Bacillus spp. were dominant amongst the isolates from the water column and sediment, while known coral pathogens responsible for coral bleaching, Vibrio coralliilyticus and Vibrio shiloi, were isolated from the coral SML and sediment samples respectively. Coral SML isolates were found to be closely related to known nitrogen fixers and antibiotic producers with tolerance towards elevated temperatures and heavy metal contamination, offering a possible explanation why the local corals are able to thrive in higher than usual temperatures. This specialized microbiota may be important for protecting the corals from pathogens by occupying entry niches and/or through the production of secondary metabolites such as antibiotics. The communities from the coral SML were tested against each other at 28, 30 and 32°C, and were also assessed for the presence of type I modular polyketides synthase (PKS) and non-ribosomal peptide synthetase (NRPS) genes which are both involved in the production of antibiotic compounds. The bacterial community from the SML exhibited antimicrobial properties under normal temperatures while pathogenic strains appeared toxic at elevated temperatures and our results highlight the role of the coral SML bacterial community in the coral’s defence.

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