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Mohammad Rifqi GHIFFARY,Cindy Pricilia Surya PRABOWO,Komal SHARMA,Sang Yup LEE,Hyun Uk KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Current industrial production of dyes for textiles mainly involves numerous toxic chemicals which causes severe water pollution. For this, indigoidine has attracted attention as an alternative natural blue dye, but it is necessary to achieve a high-level production to compete with existing synthetic blue dyes. Here, we report a metabolically engineered Corynebacterium glutamicum capable of producing indigoidine to a high concentration with high productivity. First, blue-pigment indigoidine synthetase (bpsA) gene from Streptomyces lavendulae was expressed in C. glutamicum, which already carries strong fluxes toward L-glutamate, a precursor of indigoidine. Production performance of this base strain, already producing 7.3 ± 0.3 g/L indigoidine from flask, was further improved through a series of metabolic engineering: streamlining intracellular supply of the precursors Lglutamate and L-glutamine; strengthening phosphotransferase system-independent glucose uptake system; channeling carbon fluxes from glycolysis to tricarboxylic acid (TCA) cycle; and minimizing byproducts formation. The final strain named BIRU11 produced 49.30 g/L indigoidine with the productivity of 0.96 g/L/h from fed-batch fermentation, the highest titer and productivity to date. Finally, indigoidine from the fed-batch fermentation of the BIRU11 strain was used to dye white cotton fabrics to examine its color and performance. This study demonstrates the potential of producing fabric dyes in a sustainable manner by using a metabolically engineered bacterium.
Engineering Heterologous Hosts for the Enhanced Production of Non-ribosomal Peptides
KOMAL,Mohammad Rifqi Ghiffary,김현욱,이상엽 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.6
Non-ribosomal peptides (NRPs) are a family of secondary metabolites with the highest number among entire secondary metabolite types. They are biosynthesized by a multi-modular enzyme complex called non-ribosomal peptide synthetase (NRPS), which is encoded by a biosynthetic gene cluster (BGC) in plants and a special group of microorganisms. NRPs are structurally and functionally diverse with numerous industrial applications. However, native producers of these valuable NRPs have several biotechnological limitations for efficient production, including their slow growth, inefficient genetic manipulations, and silent BGCs. Heterologous expression of NRPS can address these challenges, especially using an array of model organisms with well-studied metabolic networks and readily available genetic engineering tools. Here, we review the applications of representative bacterial heterologous hosts, namely representative model Streptomyces species, Escherichia coli, Pseudomonas putida, and Bacillus subtilis, which have been engineered for the enhanced production of NRPs
Synthetic sRNA Tool for the Targeted and High-throughput Gene Knockdown in Diverse Bacteria
Jae Sung CHO,Dongsoo YANG,Cindy Pricilia Surya PRABOWO,Mohammad Rifqi GHIFFARY,Taehee HAN,Kyeong Rok CHOI,Cheon Woo MOON,Hengrui ZHOU,Jae Yong RYU,Hyun Uk KIM,Sang Yup LEE 한국생물공학회 2023 한국생물공학회 학술대회 Vol.2023 No.10