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Modak Annayasa,Singh Brij Raj,Dubey Ashutosh,Tewari A. K.,Taj Gohar 한국작물학회 2022 Journal of crop science and biotechnology Vol.25 No.1
White rust, a devastating disease of Brassicaceae family, causes nearly 60% reduction in yield of Brassica juncea (Indian mustard). This dreadful disease is caused by oomycetes fungi Albugo candida. A comparative expression analysis of MAPKs cascade and some of defense-related genes in nontransgenic and transgenic (harbouring overexpressed MAPK3) cultivar of Brassica juncea were done to reveal the defense signalling pathway against biotrophic fungi Albugo candida. Disease indexing was also done to compare the disease severity in both transgenic and nontransgenic Brassica juncea cultivars. Lower disease index in transgenic Brassica suggests its tolerance to Albugo candida. Higher expression level of MAPK6 in comparison to MAPK3 in the transgenic Brassica indicates that MAPK6 mimics a portion of MAPK3 signalling pathway and work in a direct cascade for production of defense-related proteins. Overexpression of MAPK3 gene was observed in downregulating the expression of MAPK4 in transgenic Brassica juncea. The expression of two important transcription factors, such as WRKY33 and WRKY29 were also analyzed in the present study and were found to be higher in transgenic plant overexpressing MAPK3. The expression of three defense-related genes (OASTL-B, ACD2 and CSD2) was found to be increased in transgenic plants harbouring overexpressed MAPK3 gene; however, expression level of CYP20-3 was found higher in nontransgenic Brassica plant in response to pathogen infection. These four defense-related genes were also subjected to in silico studies, such as secondary structure, tertiary structure and putative phosphorylation sites prediction in addition to protein–protein interaction determination with potential MAPKs cascade and WRKY29, WRKY33.