Activation of K⁺ channel by 1-EBIO rescues the head and neck squamous cell carcinoma cells from Ca²⁺ ionophore-induced cell death

Ming Zhe Yin,Seok-Woo Park,Tae Wook Kang,Kyung Soo Kim,Hae Young Yoo,Junho Lee,J. Hun Hah,Myung Hun Sung,Sung Joon Kim 대한생리학회-대한약리학회 2016 The Korean Journal of Physiology & Pharmacology Vol.20 No.1

Ion channels in carcinoma and their roles in cell proliferation are drawing attention. Intracellular Ca²⁺ ([Ca²⁺]_i)-dependent signaling affects the fate of cancer cells. Here we investigate the role of Ca²⁺-activated K⁺ channel (SK4) in head and neck squamous cell carcinoma cells (HNSCCs) of dif-ferent cell lines; SNU-1076, OSC-19 and HN5. Treatment with 1 μM ionomycin induced cell death in all the three cell lines. Whole-cell patch clamp study suggested common expressions of Ca²⁺-activated Cl⁻ channels (Ano-1) and Ca²⁺-activated nonselective cation channels (CAN). 1-EBIO, an activator of SK4, induced outward K⁺ current (ISK4) in SNU-1076 and OSC-19. In HN5, ISK4 was not observed or negligible. The 1-EBIO-induced current was abolished by TRAM-34, a selective SK4 blocker. Interestingly, the ionomycin-induced cell death was effectively prevented by 1-EBIO in SNU-1076 and OSC-19, and the rescue effect was annihilated by combined TRAM-34. Con-sistent with the lower level of ISK4, the rescue by 1-EBIO was least effective in HN5. The results newly demonstrate the role of SK4 in the fate of HNSCCs under the Ca²⁺ overloaded condition. Pharmacological modulation of SK4 might provide an intriguing novel tool for the anti-cancer strategy in HNSCC.

Activation of K⁺ channel by 1-EBIO rescues the head and neck squamous cell carcinoma cells from Ca²⁺ ionophore-induced cell death

Yin, Ming Zhe,Park, Seok-Woo,Kang, Tae Wook,Kim, Kyung Soo,Yoo, Hae Young,Lee, Junho,Hah, J. Hun,Sung, Myung Hun,Kim, Sung Joon The Korean Society of Pharmacology 2016 The Korean Journal of Physiology & Pharmacology Vol.20 No.1

Ion channels in carcinoma and their roles in cell proliferation are drawing attention. Intracellular $Ca^{2+}$ ($[Ca^{2+}]_i$)-dependent signaling affects the fate of cancer cells. Here we investigate the role of $Ca^{2+}$-activated $K^+$ channel (SK4) in head and neck squamous cell carcinoma cells (HNSCCs) of dif-ferent cell lines; SNU-1076, OSC-19 and HN5. Treatment with $1{\mu}M$ ionomycin induced cell death in all the three cell lines. Whole-cell patch clamp study suggested common expressions of $Ca^{2+}$-activated $Cl^-$ channels (Ano-1) and $Ca^{2+}$-activated nonselective cation channels (CAN). 1-EBIO, an activator of SK4, induced outward $K^+$ current (ISK4) in SNU-1076 and OSC-19. In HN5, ISK4 was not observed or negligible. The 1-EBIO-induced current was abolished by TRAM-34, a selective SK4 blocker. Interestingly, the ionomycin-induced cell death was effectively prevented by 1-EBIO in SNU-1076 and OSC-19, and the rescue effect was annihilated by combined TRAM-34. Con-sistent with the lower level of ISK4, the rescue by 1-EBIO was least effective in HN5. The results newly demonstrate the role of SK4 in the fate of HNSCCs under the $Ca^{2+}$ overloaded condition. Pharmacological modulation of SK4 might provide an intriguing novel tool for the anti-cancer strategy in HNSCC.

Robot Tracking in SLAM with Masreliez-Martin Unscented Kalman Filter

Ming Tang,Zhe Chen,Fuliang Yin 제어·로봇·시스템학회 2020 International Journal of Control, Automation, and Vol.18 No.9

The simultaneous localization and mapping (SLAM) is a significant topic in intelligent robot. In this paper, a robot tracking algorithm in SLAM with Masreliez-Martin unscented Kalman filter (MMUKF) is proposed. A robot dynamic model based on SLAM characteristics is first used as state equation to model the robotic movement, and the measurement equations are deduced by linearizing the motion model. Next, the covariance of process noise is estimated with an adaptive factor to improve tracking performance in the MMUKF. Finally, the MMUKF is employed to estimate the positions of robot and landmarks. The proposed algorithm can complete robot tracking with good accuracy, and obtain reliable state estimation in SLAM. Simulation results reveal the validity of the proposed Algorithm.

An Improved Adaptive Unscented FastSLAM with Genetic Resampling

Ming Tang,Zhe Chen,Fuliang Yin 제어·로봇·시스템학회 2021 International Journal of Control, Automation, and Vol.19 No.4

The simultaneous localization and mapping (SLAM) is a significant topic in intelligent robot. In this paper, an improved adaptive unscented FastSLAM with genetic resampling is proposed. Specifically, the adaptive unscented Kalman filter (IAUKF) algorithm is improved as importance sampling of particle filter, where the adaptive factor is used to improve the tracking ability of system and the Huber cost function is constructed to decrease the measurement covariance. Next, the process noise and the measurement noise are assessed by a time varying estimator. Moreover, the resampling in particle filter is carried out by an improved genetic algorithm (GA). Finally, the improved adaptive unscented FastSLAM (IAUFastSLAM) is proposed to complete robot tracking. The proposed algorithm has good tracking performance and obtains reliable state estimation in SLAM. Simulation results reveal the validity of the proposed algorithm.

SLAM with Improved Schmidt Orthogonal Unscented Kalman Filter

Ming Tang,Zhe Chen,Fuliang Yin 제어·로봇·시스템학회 2022 International Journal of Control, Automation, and Vol.20 No.4

Simultaneous localization and mapping (SLAM) is a momentous topic for robot navigation to explore uncharted environment. To enhance the accuracy and efficiency, an improved Schmidt orthogonal unscented Kalman filter (ISOUKF) based SLAM algorithm is proposed in this paper. First, based on the Schmidt orthogonal transform (SOT) sampling, a modified unscented Kalman filter (UKF) algorithm is presented. Then, an adaptive fading factor is derived using the strong tracking algorithm, and it is introduced into the prediction covariance to improve tracking ability and accuracy. Next, the Schmidt orthogonal unscented Kalman filter is improved with square root filter to raise the efficiency of SLAM algorithm. Finally, the ISOUKF algorithm is proposed to complete the robot tracking in SLAM. The proposed algorithm provides a high precision robot tracking for SLAM and decreases the computational cost to some extent. Experiment results verify the superiority of the proposed algorithm.

An Improved UFastSLAM With Generalized Correntropy Loss and Adaptive Genetic Resampling

Ming Tang,Zhe Chen,Fuliang Yin 제어·로봇·시스템학회 2024 International Journal of Control, Automation, and Vol.22 No.3

The simultaneous localization and mapping (SLAM) is a research hotspot in robot navigation. In thispaper, an improved UFastSLAM with generalized correntropy loss and adaptive genetic resampling is proposed. Specifically, the unscented Kalman filter algorithm with generalized correntropy loss is improved as the importancesampling in particle filter. Then, an adaptive genetic algorithm is employed to complete the resampling of particlefilter. Finally, the improved UFastSLAM with generalized correntropy loss is presented to complete robot tracking. The proposed algorithm can complete robot tracking with high accuracy performance, and obtain reliable stateestimation under the non-Gaussian measurement noise in SLAM. Simulation and experiment results exhibit theavailability of the proposed SLAM algorithm.

Decreased inward rectifier and voltage-gated K+ currents of the right septal coronary artery smooth muscle cells in pulmonary arterial hypertensive rats

Sung Eun Kim,Ming Zhe Yin,Hae Jin Kim,Rany Vorn,Hae Young Yoo,Sung Joon Kim 대한생리학회-대한약리학회 2020 The Korean Journal of Physiology & Pharmacology Vol.24 No.1

In vascular smooth muscle, K+ channels, such as voltage-gated K+ channels (Kv), inward-rectifier K+ channels (Kir), and big-conductance Ca2+-activated K+ channels (BKCa), establish a hyperpolarized membrane potential and counterbalance the depolarizing vasoactive stimuli. Additionally, Kir mediates endotheliumdependent hyperpolarization and the active hyperemia response in various vessels, including the coronary artery. Pulmonary arterial hypertension (PAH) induces right ventricular hypertrophy (RVH), thereby elevating the risk of ischemia and right heart failure. Here, using the whole-cell patch-clamp technique, we compared Kv and Kir current densities (IKv and IKir) in the left (LCSMCs), right (RCSMCs), and septal branches of coronary smooth muscle cells (SCSMCs) from control and monocrotaline (MCT)- induced PAH rats exhibiting RVH. In control rats, (1) IKv was larger in RCSMCs than that in SCSMCs and LCSMCs, (2) IKv inactivation occurred at more negative voltages in SCSMCs than those in RCSMCs and LCSMCs, (3) IKir was smaller in SCSMCs than that in RCSMCs and LCSMCs, and (4) IBKCa did not differ between branches. Moreover, in PAH rats, IKir and IKv decreased in SCSMCs, but not in RCSMCs or LCSMCs, and IBKCa did not change in any of the branches. These results demonstrated that SCSMC-specific decreases in IKv and IKir occur in an MCT-induced PAH model, thereby offering insights into the potential pathophysiological implications of coronary blood flow regulation in right heart disease. Furthermore, the relatively smaller IKir in SCSMCs suggested a less effective vasodilatory response in the septal region to the moderate increase in extracellular K+ concentration under increased activity of the myocardium.