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Raj Kumar Salar,Milan Certik,Vlasta Brezova 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.1
Thamnidium elegans CCF 1456, a filamentous fungus, was used to enhance the total phenolic content and radical scavenging activity of maize via solid-state fermentation. Thamnidium fermented maize (TFM) and unfermented maize (UFM) grains were extracted with 65%ethanol and dimethylsulfoxide (DMSO). Total phenolic content (TPC), and radical scavenging capacity – determined with 1,1-diphenyl-2-picrylhydrazyl (DPPH) and radical cations of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS•+) found for TFM – were significantly (P < 0.05) higher on the 5th day of incubation than that of UFM extracts. A linear correlation was observed among TPC, DPPH and ABTS scavenging activities, and also among TPC and various carbohydrate-cleaving enzymes (α-amylase, β-glucosidase and xylanase), suggesting that this? strategy may help to enrich? TFM with improved phytochemical properties and antioxidant activities. Thamnidium elegans CCF 1456, a filamentous fungus, was used to enhance the total phenolic content and radical scavenging activity of maize via solid-state fermentation. Thamnidium fermented maize (TFM) and unfermented maize (UFM) grains were extracted with 65%ethanol and dimethylsulfoxide (DMSO). Total phenolic content (TPC), and radical scavenging capacity – determined with 1,1-diphenyl-2-picrylhydrazyl (DPPH) and radical cations of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS•+) found for TFM – were significantly (P < 0.05) higher on the 5th day of incubation than that of UFM extracts. A linear correlation was observed among TPC, DPPH and ABTS scavenging activities, and also among TPC and various carbohydrate-cleaving enzymes (α-amylase, β-glucosidase and xylanase), suggesting that this? strategy may help to enrich? TFM with improved phytochemical properties and antioxidant activities.