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      저자 : Meng-Tsan Tsai (검색결과 1 건)
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        Development of a 2-Chamber Culture System for Impedimetric Monitoring of Cell-cell Interaction

        Kin Fong Lei,Meng-Tsan Tsai,Ming-Hong Zhong,Chia-Hao Huang,Ngan-Ming Tsang,Ming-Yih Lee 한국바이오칩학회 2017 BioChip Journal Vol.11 No.2

        In cancer research, study of cell-cell interaction is important to understand tumor initiation, progression, metastasis, and therapeutic resistance. Conventionally, transwell system was adopted and cell proliferation was quantified by end-point bio-assays. The operations are labor-intensive and time-consuming while studying of the dynamic cellular responses of cell-cell interaction. Although impedance measurement was suggested to be a promising technique to monitor cellular responses, electrodes cannot be integrated into the transwell for the measurement purpose. In this work, a 2-chamber culture system incorporated with impedance measurement technique was developed to quantitatively study cell-cell interaction. The chamber was composed of 2 sub-chambers separated with a barrier. By this design, two types of cells could be independently cultured and concurrently monitored under common medium supplied. Cell-cell interaction was demonstrated by aberrant cell proliferation induced by the EGF secreted from the transfected cells cultured on another sub-chamber. Real-time and non-invasive monitoring of cell-cell interaction was successfully demonstrated. This work provides a practical solution for monitoring the dynamic cellular responses of cellcell interaction during the culture course. It is a reliable and convenient platform and facilitate more quantitative assessments in cancer research.

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