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        Multi-channel analyzer based on a novel pulse fi tting analysis method

        Qingshan Wang,Xiongjie Zhang,Xiangting Meng,Bao Wang,Dongyang Wang,Pengfei Zhou,Renbo Wang,Bin Tang 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.6

        A novel pulse fitting analysis (PFA) method is presented for the acquisition of nuclear spectra. Thecharging process of the feedback capacitor in the resistive feedback charge-sensitive preamplifier isequivalent to the impulsive pulse, and its impulse response function (IRF) can be obtained by non-linearfitting of the falling edge of the nuclear pulse. The integral of the IRF excluding the baseline representsthe energy deposition of the particles in the detector. In addition, since the non-linear fitting process inPFA method is difficult to achieve in the conventional architecture of spectroscopy system, a new multichannel analyzer (MCA) based on Zynq SoC is proposed, which transmits all the data of nuclear pulsesfrom the programmable logic (PL) to the processing system (PS) by high-speed AXI-Stream in order toimplement PFA method with precision. The linearity of new MCA has been tested. The spectrum of 137Cswas obtained using LaBr3(Ce) scintillator detector, and was compared with commercial MCA by ORTEC. The results of tests indicate that the MCA based on PFA method has the same performance as thecommercial MCA based on pulse height analysis (PHA) method and excellent linearity for g-rays withdifferent energies, which infers that PFA method is an effective and promising method for the acquisitionof spectra. Furthermore, it provides a new solution for nuclear pulse processing algorithms involvingregression and iterative processes

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        Draft Genome Assembly and Annotation for Cutaneotrichosporon dermatis NICC30027, an Oleaginous Yeast Capable of Simultaneous Glucose and Xylose Assimilation

        Wang Laiyou,Guo Shuxian,Zeng Bo,Wang Shanshan,Chen Yan,Cheng Shuang,Liu Bingbing,Wang Chunyan,Wang Yu,Meng Qingshan 한국균학회 2022 Mycobiology Vol.50 No.1

        The identification of oleaginous yeast species capable of simultaneously utilizing xylose and glucose as substrates to generate value-added biological products is an area of key eco- nomic interest. We have previously demonstrated that the Cutaneotrichosporon dermatis NICC30027 yeast strain is capable of simultaneously assimilating both xylose and glucose, resulting in considerable lipid accumulation. However, as no high-quality genome sequenc- ing data or associated annotations for this strain are available at present, it remains chal- lenging to study the metabolic mechanisms underlying this phenotype. Herein, we report a 39,305,439 bp draft genome assembly for C. dermatis NICC30027 comprised of 37 scaffolds, with 60.15% GC content. Within this genome, we identified 524 tRNAs, 142 sRNAs, 53 miRNAs, 28 snRNAs, and eight rRNA clusters. Moreover, repeat sequences totaling 1,032,129 bp in length were identified (2.63% of the genome), as were 14,238 unigenes that were 1,789.35 bp in length on average (64.82% of the genome). The NCBI non-redundant protein sequences (NR) database was employed to successfully annotate 11,795 of these unigenes, while 3,621 and 11,902 were annotated with the Swiss-Prot and TrEMBL databases, respectively. Unigenes were additionally subjected to pathway enrichment analyses using the Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Cluster of Orthologous Groups of proteins (COG), Clusters of orthologous groups for eukaryotic com- plete genomes (KOG), and Non-supervised Orthologous Groups (eggNOG) databases. Together, these results provide a foundation for future studies aimed at clarifying the mech- anistic basis for the ability of C. dermatis NICC30027 to simultaneously utilize glucose and xylose to synthesize lipids.

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