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Meishan Zhang,Ruili Lv,Wei Yang,Tiansi Fu,Bao Liu 한국유전학회 2018 Genes & Genomics Vol.40 No.1
Imprinted gene expression in flowering plants predominantly occurs in the triploid endosperm of developing seed. However, endosperm is composed of distinct tissue types. For example, the maize (Zea mays) endosperm is constituted by two major tissues, starchy endosperm and aleurone. Previous studies in imprinted gene expression have generally assumed that the different tissues constituting endosperm would behavior the same, and hence have not examined them separately. Here, to examine parentalspecific expression of imprinted genes in different parts of the seed, eight previously reported maize protein-coding imprinted genes were selected, and analyzed by cleaved amplified polymorphic sequence (CAPS) coupled with Sanger sequencing for transcripts from the various seed tissues collected at 18 days after pollination (DAP). The studied tissues included seed coat, embryo, starchy endosperm and aleurone, which were collected from a pair of reciprocal F1 hybrids produced by crossing inbred lines B73 and Mo17. Six of these eight analyzed imprinted genes showed the same imprinted expression pattern between the starchy endosperm and aleurone, but two showed imprinted expression only in the starchy endosperm. Comparison of the expression pattern of 20 selected imprinted genes in multiple seed tissues and vegetative tissues indicated that the majority (~ 75%) of these imprinted genes exhibited seedspecific or endosperm-specific expression. Our results also uncovered that imprinted genes have a high propensity to be alternatively spliced via intron retention in the developing embryo compared with the other tissues.
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Effects of methane concentration on hydrocarbon release during the pyrolysis of coal
Jianmin Zhang,Suoya,Hui Lian,Meishan Gao 한국화학공학회 2007 Korean Journal of Chemical Engineering Vol.24 No.2
weight-loss characteristics of Longkou lignite were studied by means of thermogravimetric analysisin methane ambience. Pyrolysis experiments of the sample coal in diferent concentrations of methane were carriedout on a tube reactor to study the characteristics of hydrocarbon released. The results show that methane can promotethe pyrolysis of lignite in a certain temperature range and the coal can also improve the pyrolysis of methane further.The influence of methane concentration on hydrocarbon release during the pyrolysis of coal is obvious. The hydrocar-bon released from the pyrolysis of lignite is intensive within the temperature range from 400oC to 50oC and thethat the release of C2, C3 and C4 has a close relationship with methane pyrolysis and proves that a synergistic effectdoes exist between the coal and methane.
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