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Zainuddin Nurhafizhoh,Maidin Mohd Shawal Thakib,Kamarudin Noorhazwani,Napiah Nur Robaatul Adhawiyah Mohd Ali,Keni Mohd Fahmi,Masri Mohamed Mazmira Mohd 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.1
The oil palm pest bagworm, Metisa plana, is the most crucial leaf defoliator and causes severe economic loss to the oil palm industry. Although there are available and effective pest control measures, the outbreak and infestation of the bagworms is still a recurring problem due to the lack of understanding of the pest in molecular aspect, especially with regard to insecticide resistance. Thus, in this study, we aimed to identify major genes probably involved in insecticide resistance and targets using de novo transcriptome analysis. Three major genes involved in insecticides detoxification (P450, GST and CCE) and insecticide targets that have potential for modifications (AChE, nAChR, ACCase, GABA and RyR) were identified and characterized. Transcriptome anal ysis through de novo assembly was generated using next generation sequencing (Illumina). The result generated 218,707 transcripts and 12,388 unigenes. Homology search to non-redundant (NR), nucleotide (NT) and Swis sProt databases showed that 6632 unigenes were successfully matched. A total of 80 P450s, 39 GSTs and 14 CCEs were identified. 10 unigenes that putatively encode insecticide targets were also identified. To our knowledge, this is the first exploration of the transcriptomic study underlying M. plana regarding to detoxification genes and potential insecticide targets. Therefore, the information obtained can improve the genetic understanding, especially, in the functional studies of the genes in M. plana.
Madihah Halim,Ameyra Aman-Zuki,Syarifah Zulaikha Syed Ahmad,Abdullah Muhaimin Mohammad Din,Atikah Abdul Rahim,Mohamed Mazmira Mohd Masri,Badrul Munir Md. Zain,Salmah Yaakop 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.4
Parasitoid wasps (Hymenoptera) play a significant role in reducing the pest population of the bagworm species Metisa plana. This study presents the abundance and DNA barcoding information of eight parasitoid wasps species, Dolichogenidea metesae (47%), Brachymeria carinata (19%), Buysmania oxymora (12%), Goryphus bunoh (7%), Pediobius anomalus (5%), Eupelmus cotoxanthae (2%), Apanteles aluella (5%), Apanteles sp.1 (3%) and that emerged from M. plana collected from three highly infested oil palm plantations in Selangor (west), Perak (north) and Johor (south) in Peninsular Malaysia. Samples of infested M. plana were collected from the field and reared in a rearing room. The parasitoid wasp species D. metesae recorded the highest emergence numbers and the broadest presence in all the sampling sites. The relationships among the parasitoids species were estimated and visualized using Neighbor Joining (NJ) phylogenetic analyses with the Ceraphronidae family as an outgroup. The resulting NJ tree showed that the identified parasitoid wasps were successfully classified into specific species and supported with bootstraps values between 55% to 100%. This study provides important information on potential biological control agents for M. plana that may be useful for the Malaysian oil palm industry.