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        Polyphenolic diversity and antioxidant potential of important apple (Malus domestica Borkh) cultivars

        Mansoor Sheikh,Mir Javid I.,Sharma Manish,Mir Mudasir A.,Masoodi Khalid Z.,정용석 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.5

        Apple is rich in secondary metabolites, which play a role in maintaining health and disease prevention. Polyphenols are the principal constituents of apples. In this study, 25 apple cultivars cultivated at the same field conditions were analyzed for their antioxidant potential and polyphenolic content. The total phenolic content of apples ranged from 7.22 to 143.57 mg GAE/100 g fw, the flavonoid content varied from 17.54 to 143.00 mg CE /100 g fw. The maximum catechin content 2310 ± 7.45 µg/g fw. The maximum epicatechin, quercetin and phloridzin content was observed in Malus Floribunda i.e., 1740 ± 5.03 µg/g fw, 5110 ± 4 93 µg/g fw, and 2170 ± 8.76 µg/g fw, respectively. Maximum content observed significant relations with catechin (r = 0.2943) epicatechin (r = 0.2597), quercetin (r = 0.3995), apigenin (r = 0.4054), phloridzin (r = 0.3329). The most significant correlation was observed between DPPH and apigenin (r = 0.4054). Principal component analysis and component score matrix show the distribution of polyphenolics between 25 apple cultivars of diverse origins. The HCA divided the cultivars into three main metabolically rich clusters. Our study elucidates the variation in antioxidant potential of different apple cultivars which could be useful for the development of new apple cultivars by conventional and modern breeders.

      • KCI등재

        Phenotypic, biochemical and genetic diversity of pepper (Capsicum spp.) germplasm reflects selection for cultivar types and spatial distribution

        Indrabi Sayed Azrah,Malik Ajaz,Malik Geetika,Hussain Khursheed,Shikari Asif,Mir Javid Iqbal,Park Ji Eun,Le Anh Tuan,Mansoor Sheikh,Masoodi Khalid Z. 한국식물생명공학회 2024 Plant biotechnology reports Vol.18 No.3

        Throughout the globe morphological, biochemical and genetic variability exists in chilli and is harnessed to achieve specific breeding objectives. In this study, chilli germplasm was characterized based on horticultural traits, biochemical quantifica- tion and simple sequence repeat (SSR) polymorphism for diversity estimation. A total of 36 SSR primers were utilised to study the genetic divergence among 48 genotypes of chilli collected from nine states of India. Among the 36 primers, sixteen amplified null alleles. A total of 41 alleles were detected with average 2.05 alleles per locus. The largest number of alleles (5) were obtained with marker CAMS-234. The polymorphic information content ranged from 0.06 to 0.72 with an average of 0.50. On the basis of SSR analysis, the UPGMA cluster classified 48 genotypes into three groups. There was significant variability in germplasm for all morpho-biochemical traits. Kashi Anmol (100.50 q/ha) expressed the highest yield. Highest vitamin C content at green stage was recorded in IC-561635 (187 mg/100 g) and the greatest capsaicin content (9547.90 µg/g) equivalent to pungency of 171,862.2 Scoville heat units (SHU) was recorded in Bhut Jolokia. Principal component analysis indicates that the first five principal components explain 74.63% per cent of the total variation. Additionally, analysis of molecular variance (AMOVA) showed that 1% of the total genetic variation occurred among the population and 99% genetic variation within the populations, whereas the pairwise Fst specified the moderate genetic variation ranging from 0.002 to 0.020. The present investigation has strengthened the knowledge of genetic worth of this germplasm for application in vari- ous genetic improvement programmes.

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