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- 저자 : Masatoshi Aoki (검색결과 2 건)
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인삼 조직배양에 있어 CO₂ 처리가 식물체 분화 및 생육에 미치는 영향
정찬문(Chan Moon Chung),배길관(Kil Kwan Bae),Masatoshi Aoki 한국약용작물학회 2000 한국약용작물학회지 Vol.8 No.1
This experiment was conducted to investigate the effects of length of storage period under low temperature, CO₂ enrichment and addition of plant growth regulators in Murashige and Skoog medium on the plant regeneration of Korean ginseng (Panax ginseng C.A. Meyer). Seeds were treated for 60 and 80 days respectively under 5℃ environment. 2500ppm of CO₂ was enriched by ventilation. Three plant growth regulators added to the medium were Indolbutyric acid, Benzyladenin and Gibberellic acid (GA3). The result indicated that : The capacity of differentiation was higher in the aged cotyledons from the seeds treated for 80 days under low temperature condition than in those treated for 60 days. CO₂ enrichment had stimulating effects on the growth and development of shoot primordium significantly but less effects on the formation of adventitious buds. From one zygotic embryo hundreds of plantlets were differentiated. CO₂ enrichment had effects on the formation of both indirect somatic embryo and direct somatic embryo. Indirect somatic embryo showed little growth and differentiation, being undifferentiated vascular stele and epicotyl. Direct somatic embryos were formed on the epidermis of backside basal part of cotyledon. Those embryos developed to whole plant having latent bud.
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( Kazumoto Murata ),( Masaya Sugiyama ),( Tatsuji Kimura ),( Sachiyo Yoshio ),( Tatsuya Kanto ),( Mai Asano ),( Yoshihiko Aoki ),( Tsutomu Takeda ),( Masaaki Korenaga ),( Masatoshi Imamura ),( Naohiko 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1
Background: Genetic variation of interleukin-28B (IL28B), encoding IFN-l3, predict non-responders to pegylated interferon-α/ ribavirin (Peg-IFN/RBV) therapy in chronic hepatitis C (CHC). However, it remains unknown whether IL28B is a functional phenotype, and why it failed to predict treatment responses at 20%. Methods: Message and protein levels of IFN-l3 induced by ex vivo stimulation of peripheral blood mononuclear cells (PBMC) with toll-like receptor agonists (TLR3; poly I: C, TLR7; R-837) were measured by quantitative real-time PCR and our newly developed chemiluminescence enzyme immunoassays, and compared with the clinical data. BDCA-3 or -4+dendritic cells (DC) was negatively or positively selected by Magnet-Associated Cell Sorting. Results: We firstly found that BDCA-4+DCs were the main producers of IFN-ls when stimulated with R-837 whereas BDCA-3+DCs were main producers of IFN-ls when stimulated with poly I: C, using PBMC from healthy volunteers. We also found that detectable levels of IFN-ls were inducible using small amount of PBMC by R-837, not poly I: C. When stimulated with R-837, IFN-l3 was more robustly up-regulated in the PBMC from CHC patients with favorable genotype (TT in rs8099917, n = 59) for the response to Peg-IFN/RBV than non-TT (n = 41) whereas no differences was observed in IFN-l1 or IFNl2, which may support our GWAS data. Importantly, protein levels of IFN-l3 induced by R-837 clearly differentiated the response to Peg-IFN/RBV treatment (p = 1.0×10-10), including discrepant cases such as VR in patients with TG/GG or NVR in TT genotype. Our method more accurately predicted treatment efficacies (95.7%) than IL28B genotyping (65.2%) did. Conclusions: Genetic variations in IL28B basically affect IFNl3 production, but different amount of IFN-l3 production determines the outcomes of Peg-IFN/RBV treatment. This study, for the first time, presents compelling evidence that genetic variations in IL28B confer a functional phenotype. Our method may provide more accurate prediction for the efficacy of Peg-IFN/RBV.
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