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Isabelle Ruhnke,John V. DeBiasio,Jan S. Suchodolski,Shelley Newman,Mark W. Musch,Jörg M. Steiner 대한수의학회 2014 Journal of Veterinary Science Vol.15 No.2
This study was conducted to evaluate an adapter-modifiedUssing chamber for assessment of transport physiology inendoscopically obtained duodenal biopsies from healthy catsand dogs, as well as dogs with chronic enteropathies. 17duodenal biopsies from five cats and 51 duodenal biopsiesfrom 13 dogs were obtained. Samples were transferred intoan adapter-modified Ussing chamber and sequentiallyexposed to various absorbagogues and secretagogues. Overall, 78.6% of duodenal samples obtained from catsresponded to at least one compound. In duodenal biopsiesobtained from dogs, the rate of overall response ranged from87.5% (healthy individuals; n = 8), to 63.6% (animalsexhibiting clinical signs of gastrointestinal disease andhistopathological unremarkable duodenum; n = 15), and32.1% (animals exhibiting clinical signs of gastrointestinaldiseases and moderate to severe histopathological lesions; n =28). Detailed information regarding the magnitude andduration of the response are provided. The adapter-modifiedUssing chamber enables investigation of the absorptive andsecretory capacity of endoscopically obtained duodenalbiopsies from cats and dogs and has the potential to become avaluable research tool. The response of samples was correlated with histopathological findings.