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      저자 : Mark R. Wilkins (검색결과 1 건)
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        Influence of Switchgrass Generated Producer Gas Pre-adaptation on Growth and Product Distribution of Clostridium ragsdalei

        Karthikeyan D. Ramachandriya,Mark R. Wilkins,Krushna N. Patil 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.6

        Gasification-fermentation is a thermo chemicalbiologicalprocess for the production of fuels and chemicals. Producer gas cleanup is a major issue that must beaddressed for integration of these platforms. Pre-adaptationof producer gas fermenting microbes to gas impurities hasimproved tolerances to impurities and production of alcoholsin certain bacteria. In this research, the effect of switchgrassgenerated producer gas was studied with adapted andunadapted cultures of C. ragsdalei and compared tofermentations with a control of clean custom producer gas. Results indicated no inhibition to microbial growth withunadapted cells and final cell mass concentrations were22% higher when cells were exposed to switchgrass-basedproducer gas compared to control. The ethanol productivitywith adapted cells was 1.9 and 2.8 times higher thanunadapted and control treatments, respectively. Similarly,the ethanol yield (YETOH/X) of C. ragsdalei adapted toproducer gas was 119% more than the control and 35%greater than the unadapted cells used in this study. Thepresence of switchgrass-based producer gas also inducedmetabolic shifts resulting in reduction of acetic acid toethanol that increased ethanol to acetate ratios from 0.7 g/gin control to 4.9 g/g with unadapted cells and 13.7 g/g withadapted cells. Isopropanol was also observed as a productwhen switchgrass generated producer gas was used. Weconclude that cultural adaptation of C. ragsdalei to biomassgenerated producer gas during preculture stages could beused as an important strategy to enhance ethanol yields forintegrating gasification and fermentation platforms usingC. ragsdalei.

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