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Comfort and Infection Control of Chitosan-impregnated Cotton Gauze as Wound Dressing
Jefferson M. Souza,Mariana Henriques,Pilar Teixeira,Margarida M. Fernandes,Raul Fangueiro,Andrea Zille 한국섬유공학회 2019 Fibers and polymers Vol.20 No.5
The aim of this study was to evaluate the thermo-physiological comfort properties of surgical cotton gauze coatedwith chitosan (CH) and its effectiveness for the prevention of bacterial colonization. Gauze was coated with CH at massfractions of 0.50, 0.25, 0.125, 0.10, 0.063 wt% and the friction, flexibility, thermal, moisture management and mechanicalproperties were evaluated. The best performing gauze in terms of comfort (0.125 wt%) was further evaluated for its ability toinhibit the growth of microorganisms such as bacteria and yeast. Results indicate that the functionalized medical gauze couldinduce low friction on the wound bed allowing a good degree of moisture and high absorption capacity of wound exudates. Moreover, it shows antimicrobial properties against medical-relevant pathogens. This biofunctional medical gauzedemonstrates to deliver an efficient antimicrobial coating and promote the best conditions for maintenance of the woundmicroenvironment.
( Maria Elisa Rodrigues ),( Ana Rita Costa ),( Pedro Fernandes ),( Mariana Henriques ),( Philip Cunnah ),( David W Melton ),( Joana Azeredo ),( Rosario Oliveira ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.9
The emergence of microcarrier technology has brought a renewed interest in anchoragedependent cell culture for high-yield processes. Well-known in vaccine production, microcarrier culture also has potential for application in other fields. In this work, two types of microcarriers were evaluated for small-scale monoclonal antibody (mAb) production by CHOK1 cells. Cultures (5 ml) of microporous Cytodex 3 and macroporous CultiSpher-S carriers were performed in vented conical tubes and subsequently scaled-up (20 ml) to shake-flasks, testing combinations of different culture conditions (cell concentration, microcarrier concentration, rocking methodology, rocking speed, and initial culture volume). Culture performance was evaluated by considering the mAb production and cell growth at the phases of initial adhesion and proliferation. The best culture performances were obtained with Cytodex 3, regarding cell proliferation (average 1.85 ± 0.11 × 106 cells/ml against 0.60 ± 0.08 × 106 cells/ ml for CultiSpher-S), mAb production (2.04 ± 0.41 μg/ml against 0.99 ± 0.35 μg/ml for CultiSpher-S), and culture longevity (30 days against 10-15 days for CultiSpher-S), probably due to the collagen-coated dextran matrix that potentiates adhesion and prevents detachment. The culture conditions of greater influence were rocking mechanism (Cytodex 3, pulse followed by continuous) and initial cell concentration (CultiSpher-S, 4 × 105 cells/ml). Microcarriers proved to be a viable and favorable alternative to standard adherent and suspended cultures for mAb production by CHO-K1 cells, with simple operation, easy scaleup, and significantly higher levels of mAb production. However, variations of microcarrier culture performance in different vessels reiterate the need for optimization at each step of the scale-up process.