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Qi-Xian Lian,Jian-Feng Liu,Mao-Fa Yang,Chang Han 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.3
The leafhopper Cicadella viridis (Hemiptera: Cicadellidae), a sap-sucking insect, is a notorious pest of fruit trees and seedlings. They harbor “Candidatus sulcia merii” which is an obligatory symbiotic bacteriumhoused in a special organ called the bacteriome. In this study universal eubacterial primers 27F and 1513R were used to amplify approximately 1462 bp of symbiotic bacteria 16S rRNA gene fromC. viridis. Cloning, sequencing, and phylogenetic analysis targeting the 16S rRNA gene revealed the presence of bacteria stably associated with C. viridis, and a Rickettsia endosymbiont was detected for the first time in this species. Rickettsia endosymbiont localization was studied using quantitative PCR which showed that it was found in all developmental stages. It was also co-localized in the bacteriomes, ovaries, testes, guts, and salivary glands. By an antibiotic treatment, the antibiotic-treated insects exhibited lower Rickettsia infection density than the untreated
Jian-Feng Liu,Man Liu,Mao-Fa Yang,Dimitris C. Kontodimas,Xiao-Fei Yu,Qi-Xian Lian 한국응용곤충학회 2014 Journal of Asia-Pacific Entomology Vol.17 No.4
The effect of constant temperatures on development and survival of Lista haraldusalis (Walker) (Lepidoptera:Pyralidae), a newly reported insect species used to produce insect tea in Guizhou province (China), was studiedin laboratory conditions at seven temperatures (19 °C, 22 °C, 25 °C, 28 °C, 31 °C, 34 °C, and 37 °C) on Platycaryastrobilacea. Increasing the temperature from 19 °C to 31 °C led to a significant decrease in the developmentaltime from egg to adult emergence, and then the total developmental time increased at 34 °C. Egg incubationwas the stage where L. haraldusalis experienced the highest mortality at all temperatures. The survival ofL. haraldusalis was significantly higher at 25 °C and 28 °C, whereas none of the eggs hatched at 37 °C. Commonand Ikemoto linear models were used to describe the relationship between the temperature and the developmentalrate for each immature stage of L. haraldusalis. The estimated values of the lower temperature thresholdand thermal constant of the total immature stages using Common and Ikemoto linear models were 11.34 °C and11.20 °C, and 939.85 and 950.41 degree-days, respectively. Seven nonlinear models were used to fit the experimentaldata to estimate the developmental rate of L. haraldusalis. Based on the biological significance for modelevaluation, Ikemoto linear, Logan-6, and SSI were the best models that fitted each immature stage ofL. haraldusalis and they were used to estimate the temperature thresholds. These thermal requirements and temperaturethresholds are crucial for facilitating the development of factory-based mass rearing of L. haraldusalis.
Shang-Wei Li,Fang-Chao Wei,Juan Du,Mao-Fa Yang 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.3
The rice water weevil (RWW), Lissorhoptrus oryzophilus Kuschel, is one of the most destructive pests causing heavy rice yield loss worldwide. In the present study, amplified fragment length polymorphism (AFLP) was used to analyze the genetic differentiation and transmission routes of 26 RWW geographical populations from Guizhou and 6 other provinces in China. The genetic distance between the 26 populations ranged from 0 to 0.693; the genetic distance between Guizhou and 6 other provinces populations varied from 0.043 to 0.693, with an average of 0.382. There was higher genetic variation among the populations from Guizhou and 6 other provinces compared with populations within Guizhou. The Mantel test showed no correlation between the genetic and geographical distances of the 26 populations. According to genetic distance, clustering, and time that RWW was found in these regions, we deduced that the pest entered Guizhou and spread in this province most likely via three routes: (1) from Hebei to Zunyi and southward to the south-central regions; (2) from Sichuan to Bijie and eastward to the central regions; and (3) from Yunnan through Anshun to the central regions and Qiandongnan prefecture. RWW spread in a natural manner, including flight, crawling, swimming, and by human-associated mechanisms, comprising dissemination through paddies and hay and hitchhiking on human transportations.